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Download a PDF version of my CV here (correct of 5th January 2025). Don't forget to look at my Publications.
EDUCATION & EMPLOYMENT
EDUCATION & EMPLOYMENT
Lecturer, School of Life Sciences, University of Liverpool September 2023 – Present.
Lecturer, School of Life Sciences, University of Liverpool September 2023 – Present.
Programme Director for Biomedical Sciences (C130) programme.
Link Tutor and lead moderator for XJTLU
Module organiser for LIFE221 (The Immune System in Health and Disease), LIFE365 (Advanced Skills in Biological and Medical Sciences) and LIFE204 (Endocrine and Neuro-physiology).
Involved in laboratory teaching in LIFE227 (Techniques in Cell Biology).
I supervise dissertation projects for LIFE363 (Research Project).
I am an Academic Advisor for LIFE223 (Essential Skills for the Life Sciences 2).
I am Year 3 DDC for students with surname A-H.
Lecturer in Medical Sciences, School of Medical Sciences, Bangor University April 2020 – August 2023
Lecturer in Medical Sciences, School of Medical Sciences, Bangor University April 2020 – August 2023
Teaching on C21 north Wales Graduate entry to medicine 4 year programme (MBBCh) at Bangor University in collaboration with Cardiff University’s School of Medicine
Facilitating case-based learning (CBL)
Case leading for at least three cases.
Providing lectures and tutorials on various aspects of the course.
Postdoctoral Research Associate, University of Liverpool January 2018 – April 2020
Postdoctoral Research Associate, University of Liverpool January 2018 – April 2020
The Biology of Dying: Biomarkers of prognosis in palliative lung cancer patients. Wellcome Trust seed award in science & North West Cancer Research short impact grant to investigate the biology of dying & Method optimisation of urine GC-MS analysis. Whilst there are common themes to the physiology and biochemistry of dying shared amongst advanced cancer patients, indicative of a common biological process to dying, there are no clear biomarkers of prognosis. Providing a more accurate prognosis is essential to providing the best care possible to patients and their families at a difficult time. Using urine samples collected from patients in the last weeks and days of life of lung cancer, this project examines volatile organic compounds (VOCs) in urine from as a potential prognosticator by gas chromatography-mass spectrometry (GC-MS).
Letter: Chapman, E., & Coyle, S. (n.d.). Predicting the last days of life will change clinical practice. BMJ Supportive and Palliative Care. doi:10.1136/bmjspcare-2020-002187].
Poster presentation: Chapman E, et al. Volatile organic compounds as biomarkers of prognosis in palliative lung cancer patients. Metabolomics 2019, The Hague, The Netherlands, June 23-27, 2019
Poster presentation: Coyle S, Chapman E, et al. Volatile organic compounds predict the last week of life in lung cancer patients. APM, March 2019, https://spcare.bmj.com/content/9/Suppl_1/A27.2.abstract
Oral presentation: European Association for Palliative Care (EAPC) 23 - 25 May 2019, Berlin, Germany
Postdoctoral Research Associate, University of Liverpool June 2016 – Nov 2017
Postdoctoral Research Associate, University of Liverpool June 2016 – Nov 2017
Proteomic analysis of neutrophil extracellular traps (NETs) in rheumatoid arthritis. Wellcome Trust Seed Award in Science to investigate the proteins contained in Neutrophil Extracellular Traps (NETs) in auto-immune diseases, systemic lupus erythematosus (SLE) and rheumatoid arthritis (RA). NETs are chromatin structures covered in anti-microbial proteins that are released by neutrophils in response to infection. The exposure of proteins and DNA within NETs may also trigger auto-immune responses, e.g. SLE or RA, where auto-antibodies to double-stranded DNA (SLE) and citrullinated proteins (RA) may contribute to disease pathology. The aim of this work was to perform quantitative proteomics of SLE and RA NETs.
Journal article: Chapman, E., Lyon, M., Simpson, D., Mason, D., Beynon, R., Moots, R., & Wright, H. L. Caught in a Trap? Proteomic analysis of neutrophil extracellular traps in rheumatoid arthritis and systemic lupus erythematosus. Frontiers in Immunology. https://doi.org/10.3389/fimmu.2019.00423
Review Article: Glennon-Alty L*, Hackett AP*, Chapman EA*, Wright HL. *Contributed equally. Neutrophils and redox stress in the pathogenesis of autoimmune disease. Free Radic Biol Med. 2018 Mar 29. pii: S0891-5849(18)30159-X. https://doi.org/10.1016/j.freeradbiomed.2018.03.049
Conference Abstracts: Chapman EA, Lyon M, Simpson D, Beynon RJ, Moots RJ, Wright HL. Proteomic analysis of neutrophil extracellular traps. The Neutrophil International Symposium 2018; Quebec (oral presentation) http://theneutrophil.com/wp-content/uploads/2018/05/Short-program.pdf
Science Technician, St Julie's Catholic High School, Woolton Jan 2016 - May 2016
Science Technician, St Julie's Catholic High School, Woolton Jan 2016 - May 2016
Sourcing and preparing reagents and equipment for class demonstrations and practicals. Inspiring students to consider a career in science.
PhD, Haematology & Leukaemia, University of Liverpool Jan 2012 – Dec 2016
PhD, Haematology & Leukaemia, University of Liverpool Jan 2012 – Dec 2016
“The role and regulation of AKT in CD40-mediated survival and proliferation of CLL cells.”
Supervisors: Dr Jack Zhuang, Professor A. Pettitt, University of Liverpool & Dr B.R.Davies, AstraZeneca
AstraZeneca sponsored PhD, involving pre-clinical trialling of novel drug compounds in primary patient samples to gain an understanding into role of AKT in CD40-induced survival and proliferation of CLL cells.
Casual Chronic Lymphocytic Leukaemia (CLL) Biobank Technician. Oct 2015 – Nov 2015
Casual Chronic Lymphocytic Leukaemia (CLL) Biobank Technician. Oct 2015 – Nov 2015
Line Manager: Dr Melanie Oates.
Team work to process patient blood samples in clinical trials for biobank storage to GCP.
CRUK Laboratory Technician (Research) Grade 5, University of Liverpool. Oct 2010– Dec 2011
CRUK Laboratory Technician (Research) Grade 5, University of Liverpool. Oct 2010– Dec 2011
Line Manager: Dr Triantafillos Liloglou & Principal Investigator: Professor John Field
DNA isolation from a variety of clinical specimens (bronchial washings and sputum) and assay development for real-time quantitative specific methylation PCR (qMSP) in order to develop an assay for early detection of lung cancer.
MPhil, Cancer Studies, University of Wales, Bangor Oct 2008 – Sept 2010
MPhil, Cancer Studies, University of Wales, Bangor Oct 2008 – Sept 2010
(Undertaken at Department of Molecular and Clinical Pharmacology, University of Liverpool)
“Proteomic analysis of VEGF-induced endothelial cell proliferation and differentiation.”
Supervisors: Dr Michael Cross & Dr Jane Wakeman
Using Antibody arrays and iTRAQ (Isobaric tags for relative and absolute quantitation) to understand the proteomics of VEGF-induced endothelial cell proliferation and differentiation upon gelatin and collagen respectively.
Nuffield Foundation Summer Research Studentship. Summer 2007 (8 weeks)
Nuffield Foundation Summer Research Studentship. Summer 2007 (8 weeks)
North West Cancer Research Fund Institute, University of Wales, Bangor.
Supervisor: Dr Michael Cross
cDNA synthesis & SYBR green qPCR to investigate RCAN1.4 gene expression upon VEGF stimulation of endothelial cells.
BA (Hons) Natural Sciences, Biochemistry (2i) (2008) Oct 2005 – June 2008
BA (Hons) Natural Sciences, Biochemistry (2i) (2008) Oct 2005 – June 2008
Selwyn College, University of Cambridge.
Part II: Biochemistry (2008)
Specialist modules: ‘Diabetes and Obesity,’ and ‘Control of gene expression’.
Lab project (8 weeks) ‘RNA localisation in Schizosaccharomyces pombe’ where I performed the specialised lab methods RIp chip (RNA immunoprecipitation followed by DNA microarrays ‘chips’).
Supervised by Dr. Juan Mata
Part 1B: Chemistry B (Organic and Inorganic), Biochemistry, Cell Developmental Biology. (2007)
Part 1A: Biology of Cells, Chemistry, Geology, Maths (2006)
Ysgol Aberconwy, Conwy Sept 1998 – June 2005
Ysgol Aberconwy, Conwy Sept 1998 – June 2005
A level Biology (A) (2005) Chemistry (A) (2005) Physics (A) (2005) Maths (A) (2005)
AS' Level German A (2004) Welsh (2nd language) A (2003)
GCSE's 8 x A*s and 2 x As (2003)
Here is my Scopus list of publications.
On PubMed, you'll only find my publications.
2020
2020
Chapman, E., & Coyle, S. (n.d.).
Predicting the last days of life will change clinical practice.
BMJ Supportive and Palliative Care. doi:10.1136/bmjspcare-2020-002187
PubMed article | BMJ Supportive and Palliative Care | Liverpool Repository | Download a PDF (unformatted, but accepted)|
2019
2019
Chapman, E. A., Lyon, M., Simpson, D., Mason, D., Beynon, R. J., Moots, R. J., & Wright, H. L.
Caught in a Trap? Proteomic Analysis of Neutrophil Extracellular Traps in Rheumatoid Arthritis and Systemic Lupus Erythematosus.
FRONTIERS IN IMMUNOLOGY, 10:423. (11 March 2019) DOI: 10.3389/fimmu.2019.00423
PubMed article | PubMed Central article | Frontiers in Immunology online | Download a PDF.
2018
2018
Glennon-Alty L*, Hackett AP*, Chapman EA*, Wright HL.
Neutrophils and redox stress in the pathogenesis of autoimmune disease.
Free Radic Biol Med. 2018 Mar 29. pii: S0891-5849(18)30159-X. https://doi.org/10.1016/j.freeradbiomed.2018.03.049
PubMed article | Free Radic Biol Med. online | Download a PDF.
*Joint first author. Contributed equally. I authored the NETs, SLE and APS sections. I also helped offer significant and minor edits to the final draft.
2017
2017
Elinor Chapman, Melanie Oates, Ishaque Mohammad, Barry Davies, Paul Stockman, Andrew Pettitt, Jianguo Zhuang. (2017).
Delineating the distinct role of AKT in mediating cell survival and proliferation induced by CD154 and IL-4/IL-21 in chronic lymphocytic leukemia Oncotarget, Accepted: 26/10/2017 | https://doi.org/10.18632/oncotarget.22292
PubMed article | PubMed Central article | Oncotarget online | Download a PDF.
2016
2016
My PhD Thesis
Chapman, E. A. (2016). The role and regulation of AKT in CD40-mediated survival and proliferation of CLL cells.(University of Liverpool).
2015
2015
Abstracts
Zhuang, J., Chapman, E., Stockman, P., Davies, B., & Pettitt, A. (2015). Distinct requirement of AKT for CD40-induced cell growth and division in chronic lymphocytic leukemia. LEUKEMIA & LYMPHOMA, 56, 16-18.
Abstracts at XVI International Workshop on Chronic Lymphocytic Leukemia 2015 6–9 September 2015 Sydney, Australia
Retrieved from http://gateway.webofknowledge.com/
http://www.tandfonline.com/toc/ilal20/56/sup1 |Download PDF of abstracts (page 17/167).
2013
2013
Poster Abstracts
International Workshop on CLL (iwCLL) Cologne, German.
9-11 September 2013: Poster 3.3 “The novel Akt inhibitor AZD5363 enhances cytotoxicity of bendamustine in CD40-stimulated primary chronic lymphocytic leukaemia cells and prevents leukemic cells from proliferating in response to CD40/IL-4 stimulation”.
Download a PDF of all the abstracts at iwCLL 2013 (page 75/267).
2010
2010
Journal article
Holmes, K., Williams, M. C., Chapman, E. A., & Cross, M. J. (2010). Detection of siRNA induced mRNA silencing by RT-
qPCR: considerations for experimental design. BMC Research Notes, 3, 53.
PubMed article | PubMed Central article | BMC Research Notes | Download a PDF.
Journal article
Holmes, K., Chapman, E., See, V., & Cross, M. J. (2010). VEGF Stimulates RCAN1.4 Expression in Endothelial Cells via a Pathway Requiring Ca2+/Calcineurin and Protein Kinase C-δ. PLoS ONE, 5(7), e11435. doi:10.1371/journal.pone.0011435
PubMed article | PubMed Central Article | PLoS One website. | Download a PDF.
2009
2009
Abstracts
15-17th July 2009: The Biochemical Society Molecular and cellular mechanisms of angiogenesis, Chester. P034 The role of RCAN1 in VEGFR-2-mediated angiogenesis Katherine Holmes, Elinor Chapman, Michael Cross Dept of Pharmacology and Therapeutics, University of Liverpool, L79 3GE http://www.biochemistry.org/Portals/0/Conferences/abstracts/SA102/SA102P034.pdf
TECHNICAL SKILLS AND EXPERTISE
TECHNICAL SKILLS AND EXPERTISE
GCMS & VOCs analysis: Perkin Elmer’s Clarus® 500 GC/MS. AMDIS software.
Cell based assays: clinical sample handling (tissue and fluids), blood fractionation and cell separation/isolation, using HetaSep and Ficoll Paque density centrifugation or lymphoprep, negative selection of cells using magnetic beads; primary and cell line culture, co-culture systems, cell culture drug assays (BrdU assay, propidium iodide and CFSE proliferation assays), Apoptosis assays – Annevin V-PI, Flow cytometry. ROS assays (luminol, isoluminol and DHR); Generation of immune complexes; NET assays – by SYTOX green plate reader, micrococcal nuclease digestion of supernatant DNA and by immunofluorescence (IF).
Protein based work: SDS-PAGE, coomassies & immunoblotting (western blotting), immunoprecipitation, antibody microarrays, iTRAQ, flow cytometry, protein isolation using strataclean beads, generation of proteomics data and proteomics data analysis. Interpretation and analysis of large datasets, including using PANTHER gene analysis. Immunofluorescence (IF) staining; Use of wide-field EPI fluorescent microscope.
DNA/RNA work: RIp chip, DNA and RNA isolation, cDNA synthesis & SYBR green qPCR, bisulphite treatment of DNA, primer & probe design and optimisation, quantitative specific methylation PCR.
Practical medical training: Venepuncture training. Basic life skills training.
Interactions with NHS partners: NHS research passport, training on Aintree University Hospital system SIGMA to obtain patient clinical information, collaboration with research nurses, phoning patients for blood donation, consenting patients for study. I hold a NHS e-certificate in Good Clinical Practice (Secondary Care).
Computing skills: Microsoft Office: Word, Powerpoint, Excel. EndNote Reference Manager. FlowJo (flow cytometer software). Basic statistical analysis using SPSS. PANTHER classification. ImageJ. Perseus. ‘R’ programming to analyse proteomic and metabolomics datasets.
General: Team worker & collaborator (with colleagues and pharmaceutical industry), confident presenter and speaker, contribute to discussions in lab group meetings and journal club. Assisted with a two lab relocations. Managing grant allocation.
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