Tissue imaging using Hyperion imaging mass cytometry (IMC)
Tissue imaging using Hyperion imaging mass cytometry (IMC)
Embedded Files
Welcome to the web resource for the Hyperion imaging mass cytometer (IMC) within the University of Manchester, UK. The purpose of this page is not to give an exhaustive understanding of imaging mass cytometry (for that, visit Fluidigm's Hyperion website), but as a training resource for those new to the technology, and that may want to use it in their research. Enquiries for using the technology should go through the facility manager, Gareth Howell.
Technology overview
Technology overview
Tissue section is labelled with upwards of 35 antibodies, with each antibody conjugated with a different metal.
UV laser ablates the tissue in individual 1 um2 pixels.
Resulting ‘plume’ of ablated tissue passes through a plasma source, ionising it completely into its constituent atoms.
Time-of-flight mass spectrometry discriminates the signal for each of the metal-conjugated probes.
Images for each antibody are reconstructed based off the metal abundancy at each pixel.
Cell-segmentation of the resulting multiplexed images allows single-cell marker expression to be quantified, ready for downstream analysis.
Google Sites
Report abuse