Armindo Salvador CV

Ph. D., Theoretical Biochemistry (The University of Lisbon, Portugal, 1997) 

2024- Principal Investigator, Computational & Systems Biology, Center for Neuroscience and Cell Biology - The University of Coimbra, Portugal

2005-2023       Group leader, Computational & Systems Biology, Center for Neuroscience and Cell Biology - The University of Coimbra, Portugal

2011-2013  Invited Professor, The University of Coimbra, Portugal

2002-2005  Post-doctoral fellow, Molecular Systems Biology, The University of Coimbra, Portugal

2002           Visiting scholar, Molecular Systems Biology, University of California - Davis, U.S.A. 

2000-2002  Research fellow, Molecular Systems Biology, Pfizer Global Research & Development/The University of Michigan, U.S.A.

1998-2000  Post-doctoral fellow, Computational Systems Biology, The University of Michigan, U.S.A.

1997-1998  Post-doctoral fellow, Membrane biophysics, Institute for Chemical and Biological Technology, Portugal

Main research interests

• Organization principles of biochemical systems

• Redox signaling

• Metabolism

Other activities

• President, Portuguese Biophysical Society

• Member of the Consulting Committee, Portuguese Biochemical Society

• Editorial Board, Journal of theoretical Biology

• Coordinator, Summer School in Computational Biology - from molecules to tissues

Representative publications

Peskin, A. V., Meotti, F. C., Magon, N. J., de Souza, L. F. , Salvador, A.*, Winterbourn, C. C.* (2025). “Mechanism of Glutathionylation of the Active Site Thiols of Peroxiredoxin 2.” Journal of Biological Chemistry, 108503. doi: 10.1016/j.jbc.2025.108503

Zimmermann, J., Lang, L., Malo Pueyo, J., Riedel,  M., Wahni, K., Stobbe, D., Lux, C., Janvier, S., Vertommen, D., Lenhard, S., Hannemann, F., Castro, H., Tomas, A. M., Herrmann, J. M., Salvador, A., Mühlhaus, T., Riemer, J., Messens, J., Deponte, M., Morgan, B. (2025). “Heterooligomerization Drives Structural Plasticity of Eukaryotic Peroxiredoxins.” BioRxiv. doi: 10.1101/2025.03.03.641172.

Griffith, M., Araújo, A.*, Travasso, R.*, Salvador, A.* (2024). The architecture of redox microdomains: Cascading gradients and peroxiredoxins’ redox-oligomeric coupling integrate redox signaling and antioxidant protection. Redox Biology, 103000. doi: 10.1016/j.redox.2023.103000

Salvador, A. (2024). Pillars of theoretical biology: “Biochemical systems analysis, I, II and III,” Journal of Theoretical Biology 576:111655. doi:10.1016/j.jtbi.2023.111655.

Antunes, F., Salvador, A. (2023). How Gradients and Microdomains Determine H2O2 Redox Signaling. In I. Medraño-Fernández, G. P. Bienert, & R. Sitia (Eds.), Peroxiporins (pp. 5–20). Boca Raton: CRC Press. doi: 10.1201/9781003160649-2

Ferreira, M. J., Rodrigues, T. A., Pedrosa, A. G., Gales, L., Salvador, A., Francisco, T., Azevedo, J. E. (2023). The mammalian peroxisomal membrane is permeable to both GSH and GSSG – Implications for intraperoxisomal redox homeostasis. Redox Biology, 102764. doi: 10.1016/j.redox.2023.102764

Moreno, M. J.*, & Salvador, A. (2023). Ligand’s Partition to the Lipid Bilayer Should Be Accounted for When Estimating Their Affinity to Proteins. Molecules, 28(7), 3136. doi: 10.3390/molecules28073136

Sousa, T., Gouveia, M., Travasso, R. D. M.*, & Salvador, A.* (2022). How abundant are superoxide and hydrogen peroxide in the vasculature lumen, how far can they reach? Redox Biology, 102527. doi: 10.1016/j.redox.2022.102527 (SPBf Young Biophysicist 2023 prize)

Moreno, M. J.*, Loura, L. M. S., Martins, J., Salvador, A., Velazquez-Campoy, A. (2022). Analysis of the Equilibrium Distribution of Ligands in Heterogeneous Media: Approaches and Pitfalls. International Journal of Molecular Sciences, 23(17), 9757. doi: 10.3390/IJMS23179757

Oliveira, A. C., Filipe, H. A. L., Ramalho, J. P. P., Salvador, A., Geraldes, C. F. G. C., Moreno, M. J.*, Loura, L. M. S.* (2022). Modeling Gd3+ Complexes for Molecular Dynamics Simulations: Toward a Rational Optimization of MRI Contrast Agents. Inorganic Chemistry, 61(30), 11837–11858. doi: 10.1021/acs.inorgchem.2c01597

Lee, H. Y., Ithnin, A., Azma, R. Z., Othman, A., Salvador, A., Cheah, F. C. (2022). Glucose-6-Phosphate Dehydrogenase Deficiency and Neonatal Hyperbilirubinemia: Insights on Pathophysiology, Diagnosis, and Gene Variants in Disease Heterogeneity. Frontiers in Pediatrics, 10, 795. DOI: 10.3389/fped.2022.875877

Cordeiro, M.M., Salvador, A.*, Moreno, M.J.* (2022). Calculation of Permeability Coefficients from Solute Equilibration Dynamics: An Assessment of Various Methods. Membranes, 12, 254. DOI: 10.3390/membranes12030254

Ledo, A., Fernandes, E., Salvador, A., Laranjinha, J., Barbosa, R.M. (2022). "In vivo hydrogen peroxide diffusivity in brain tissue supports volume signaling activity". Redox Biology, 50, 102250 DOI:10.1016/j.redox.2022.102250.

Peskin, A.V., Meotti, F.C.; Sousa, L.F., Anderson, R.F., Winterbourn, C.C.*, Salvador, A.* (2020). "Intra-dimer cooperativity between the active site cysteines during the oxidation of peroxiredoxin 2". Free Radical Biology and Medicine 158:115-125. DOI:10.1016/j.freeradbiomed.2020.07.007 [Full text][Formatted full text]

Filipe, H. A. L., Javanainen, M., Salvador, A., Galvão, A. M., Vattulainen, I., Loura, L. M. S., Moreno, M. J. (2018) "Quantitative assessment of methods used to obtain rate constants from molecular dynamics simulations – translocation of cholesterol across lipid bilayers". J. Chem. Theory Comput. 14:3840–3848. DOI: 10.1021/acs.jctc.8b00150.

Selvaggio, G., Coelho, P.M.B.M., Salvador, A. (2018) "Mapping the phenotypic repertoire of the cytoplasmic 2-Cys peroxiredoxin - thioredoxin system. 1. Understanding commonalities and differences among cell types". Redox Biology  15:297-315. DOI:10.1016/j.redox.2017.12.008 [Full text]

Travasso, R.D.M., Sampaio dos Aidos, F., Bayani, A., Abranches, P., Salvador, A. (2017). "Localized Redox Relays as a Privileged Mode of Cytoplasmic Hydrogen Peroxide Signaling". Redox Biology 12:233–245. DOI:10.1016/j.redox.2017.01.003. [PDF]

Miranda-Santos, I., Gramacho, S., Pineiro, M., Martinez-Gomez, K., Fritz, M., Hollemeyer, K., Salvador, A., Heinzle, E. (2015). "Mass isotopomer analysis of nucleosides isolated from RNA and DNA using GC-MS" Anal. Chem. 87, 617-623.

Benfeitas, R., Selvaggio, G., Antunes, F., Coelho, P. M. B. M., Salvador, A. (2014). "Hydrogen peroxide metabolism and sensing in human erythrocytes: a validated kinetic model and reappraisal of the role of peroxiredoxin II." Free Radic. Biol. Med. 74:35-49. [PDF]

Filipe, H., Salvador, A., Silvestre, J., Vaz, W., Moreno, M.J. (2014). "Beyond Overton’s rule - Quantitative Modeling of Passive Permeation Through Tight Cell Monolayers" Molec. Pharmaceut. 11:3696-706.

Estronca, L. M. B. B., Filipe, H. A. L., Salvador, A., Moreno, M. J., & Vaz, W. L. C. (2014). "Homeostasis of Free Cholesterol in the Blood - a Preliminary Evaluation and Modeling of Its Passive Transport." J. Lipid Res. 55:1033-1043. [Abstract][PDF]

Dehmer, M., Emmert-Streib, F., Graber, A., Salvador, A. (Ed.s) (2011) “Applied Statistics for Network Biology”, Weinheim: Wiley.

Coelho, P. M. B. M., Salvador, A., Savageau, M. A. (2010). “Relating Mutant Genotype to Phenotype via Quantitative Behavior of the NADPH Redox Cycle in Human Erythrocytes” PLoS ONE 5:e13031 [Full text] [PDF]

Savageau, M.A., Coelho, P.M.B.M., Fasani, R.A., Tolla, D.A., Salvador, A. (2009). Phenotypes and tolerances in the design space of biochemical systems. Proc. Natl. Acad. Sci. USA 106: doi:10.1073/pnas.0809869106 [Abstract] [PDF]

Coelho, P. M. B. M., Salvador, A., Savageau, M. A. (2009). "Quantifying Global Tolerance of Biochemical Systems: Design Implications for Moiety-Transfer Cycles." PLoS Comp. Biol. 5:e1000319. [Abstract] [PDF] [PLoS Comput. Biol.]

Salvador, A. (2008). “Book review: Uri Alon (2008). ‘An introduction to systems biology: design principles of biological circuits’ London: Chapman & Hall/CRC”, Math. Biosci. 215:193-195. [Math. Biosci.]

Alves, R., Antunes, F., Salvador, A. (2006). Tools for kinetic modeling of biochemical systems, Nature Biotechnol. 24:667-672. [Abstract] [PDF] [Nature Biotechnology]

Gardner, R., Moradas-Ferreira, P., Salvador, A. (2006). Why does superoxide dismutase overexpression often increase hydrogen peroxide concentrations? An alternative explanation, J. Theor. Biol. 242:798-800. [PDF] [J. Theor. Biol.]

Salvador, A., Savageau, M. (2006) Evolution of enzymes in a series is driven by dissimilar functional demands. Proc. Natl. Acad. Sci. USA 103:2226-2231. [Abstract] [PDF] [PNAS]

Salvador, A. and Savageau, M. A. (2003). Quantitative evolutionary design of glucose 6-phosphate dehydrogenase expression in human erythrocytes. Proc. Natl. Acad. Sci. USA 100:14463-14468. [Abstract] [PDF]

Gardner, R., Salvador, A., and Moradas-Ferreira, P. (2002). Why does SOD overexpression sometimes enhance, sometimes decrease, hydrogen peroxide production? A minimalist explanation. Free Radic. Biol. Med. 32:1351-1357. [Abstract] [PDF]

Salvador, A., Sousa, J., and Pinto, R. E. (2001). Hydroperoxyl, superoxide and pH gradients in the mitochondrial matrix: a theoretical assessment. Free Radic. Biol. Med. 31:1208-1215. [Abstract] [PDF]

Salvador, A. (2000). Synergism analysis of metabolic processes: I. Conceptual framework. Math. Biosci. 163:105-129. [Abstract] [PDF]

Salvador, A. (2000). Synergism analysis of metabolic processes: II. Tensor formulation and treatment of stoichiometric constraints. Math. Biosci. 163:131-158. [Abstract] [PDF]

Antunes, F., Salvador, A., Marinho, H. S., Alves, R., and Pinto, R. E. (1996). Lipid peroxidation in mitochondrial inner membranes. I. An integrative kinetic model. Free Radic. Biol. Med. 21:917-943. [Abstract] [PDF]

Antunes, F., Salvador, A., and Pinto, R. E. (1995). PHGPx and phospholipase A2/GPx: comparative importance on the reduction of hydroperoxides in rat liver mitochondria. Free Radic. Biol. Med. 19:669-677. [Abstract] [PDF]

Salvador, A., Antunes, F., and Pinto, R. E. (1995). Kinetic modelling of in vitro lipid peroxidation experiments -- 'low level' validation of a model of in vivo lipid peroxidation. Free Radic. Res. 23:151-172. [Abstract] [preprint]