Publications
Liu, D.D. and Cheow, L.F.(2023) Rapid Information Retrieval from DNA Storage with Microfluidic Very Large-Scale Integration Platform. Small, 2309867. link
Viswanathan, R., Cheruba, E., Wong, P.M., Yi, Y., Ngang, S., Chong, D.Q., Loh, Y.H., Tan, I.B. & Cheow, L.F. (2023). DARESOME enables concurrent profiling of multiple DNA modifications with restriction enzymes in single cells and cell-free DNA. Science Advances, 9(37), eadi0197 link
Luah, Y. H., Wu, T., & Cheow, L. F. (2023). Identification, sorting and profiling of functional killer cells via the capture of fluorescent target-cell lysate. Nature Biomedical Engineering link .
Wu, T.J., Tan, J.H.L., Sin, W.X., Luah, Y.H., Tan, S.Y., Goh, M., Birnbaum, M.E., Chen, Q., & Cheow, L.F. (2023). Cell Granularity Reflects Immune Cell Function and Enables Selection of Lymphocytes with Superior Attributes for Immunotherapy. Advanced Science, 2302175 link
Liu, D. D., Muliaditan, D., Viswanathan, R., Cui, X., & Cheow, L. F. (2023). Melt‐Encoded‐Tags for Expanded Optical Readout in Digital PCR (METEOR‐dPCR) Enables Highly Multiplexed Quantitative Gene Panel Profiling. Advanced Science, 2301630 link
Cui, X., Ngang, S., Liu, D. D., & Cheow, L. F. (2023). Rapid Single‐Round Pool Testing of Infectious Disease Enabled by Multicolor Digital Melting PCR. Small, 2205636 link
Wu, T.J., Womersley, H.J., Wang, J., Scolnick, J., Cheow, L.F. (2023) Time-resolved assessment of single-cell protein secretion by sequencing. Nature Methods, 1-12. link Research briefing GenomeWeb Press release
Cheruba, E., Viswanathan, R., Wong, P.M., Womersley, H.J., Han, S., Tay, B., Lau, Y., Gan, A., Poon, S.Y., Skanderup, A., Ng, S.B., Chok, A.Y., Chong, D.Q., Tan, I.B., Cheow, L.F. (2022) Heat selection enables highly scalable methylome profiling in cell-free DNA for noninvasive monitoring of cancer patients. Science Advances 8(36) eabn4030 link Press release Interview with Channel News Asia Lianhe Zaobao
Luo, Y., Cui, X., Cheruba, E., Chua, Y.K., Ng, C., Tan, R.Z., Tan, K.K, Cheow, L.F. (2022) SAMBA: A Multicolor Digital Melting PCR Platform for Rapid Microbiome Profiling. Small Methods e0022185 link
Joanito, I. et. al., (2022) Single-cell and bulk transcriptome sequencing identifies two epithelial tumor cell states and refines the consensus molecular classification of colorectal cancer. Nature Genetics 54(7) 963-975 link
Lin, W.N., Tay, M.Z., Wong, J.X.E., Lee, C.Y., Fong, S.W., Wang, C., Ng, L.F.P., Renia, L., Chen, C.H., Cheow, L.F. (2022) Rapid microfluidic platform for screening and enrichment of cells secreting virus neutralizing antibodies. Lab Chip 22 (13) 2578-2589 link
Cheow, L.F. (2022) Single cell analysis technologies in biomedical research. SLAS Technology 27(2) 107-108 link
Kathrada, A.I., Wei, S.C., Xu, Y., Cheow, L.F., Chen, C.H. (2020). Microfluidic compartmentalization to identify gene biomarkers of infection Biomicrofluidics 14(6) 061502 link
Kwek, K.Z., Lim, S.L., Lu, R., Woo, K.L., Sun, G., Quek, K.Y., Cheow, L.F., Chen, C.H., Han, J. (2021). Multiplexed single-cell leukocyte enzymatic secretion profiling from whole blood reveals patient specific immune signature. Analytical Chemistry 93(10) 4374-4382 link
Luo, Y. Viswanathan, R., Hande, M.P., Loh, A.H.P, Cheow, L.F. (2020) Massively parallel single-molecule telomere length measurement with digital real-time PCR. Science Advances 6 (34), eabb7944 link Press release, Featured in NUS Engineering,Interview with CNA938
Lin, W.N., Tay, M.Z., Lu, R., Liu, Y., Chen, C.H., Cheow, L.F. (2020) The role of single-cell technology in the study of infectious diseases. Cells 9 (6), 1440 link
Sun, G., Teng, Y., Zhao, Z., Cheow, L.F., Yu, H, Chen, C.H. (2020) Functional stem cell sorting via integrative droplet synchronization. Anal. Chem. 92(11) 7915-7923 link
Viswanathan, R., Cheruba, E., Cheow, L.F. (2019) DNA Analysis by Restriction Enzyme (DARE) enables concurrent genomic and epigenomic characterization of single cells. Nucleic Acids Research. gkz717 link
Teo, A.K.K., Lim, C.S., Cheow, L.F., Kin, T., Shapiro, J.A., Kang, N.Y., Burkholder, W, Lau, H.H. (2018) Single-cell analyses of human islet cells reveal de-differentiation signatures. Cell Death Discov. 4:14 link
Ling, K.Y., Cheow, L.F., Quake, S.R., Burkholder, W.F., Messerschmidt, D.M (2017) Single cell restriction enzyme-based analysis of methylation at genomic imprinted regions in preimplantation mouse embryos. Methods Mol Biol. 1605:171-189 link
Cheow, L.F., Courtoise, E.T., Tan, Y., Viswanathan, R., Xing, Q., Tan, D.S.W., Robson, P., Loh, Y.-H., Quake, S.R., Burkholder, W.F. (2016) Single cell multimodal profiling reveals cellular epigenetic heterogeneity. Nature Methods. 13: 933-836 (corresponding author) link
Cheow, L.F., Quake, S.R., Burkholder, W.F., and Messerschimdt, D.M. (2015). Multiplexed locus-specific analysis of DNA methylation in single cells. Nature Protocols. 10(4): 619-631 link
Cheow, L.F., Sarkar, A, Kolitz, S., Lauffenburger, D., and Han, J. (2014). Detecting kinase activities from single cell lysate using concentration-enhanced mobility shift assay. Anal. Chem. 86(15): 7455-7462 link
Cheow, L.F., Viswanathan, R., Chin, C.S., Jennifer, N., Jones, R.C., Guccione, E., Quake, S.R., and Burkholder, W.F. (2014). Multiplexed analysis of protein-ligand interactions by fluorescence anisotropy in a microfluidic platform. Anal. Chem. 86(19): 9901-9908 link
Lorthongpanich, C.*, Cheow, L.F.*, Balu, S., Quake, S.R., Knowles, B.B., Burkholder, W.F., Solter, D, and Messerschmidt, D.M. (2013). Single-cell DNA methylation analysis reveals epigenetic chimerism in preimplantation embryos. Science.341(6150): 1110-1112 * equal contribution link
Cheow, L.F., Bow, H., and Han, J. (2012). Continuous-flow biomolecule concentration and detection in a slanted nanofilter array. Lab Chip. 12: 4441-4448 link
Cheow, L.F., and Han, J. (2011). Continuous signal enhancement for sensitive aptamer affinity probe electrophoresis assay using electrokinetic concentration. Anal. Chem. 83(18): 7086-7093 link
Ko, S.H., Kim, S.J., Cheow, L.F., Li, L.D., Kang, K.H., and Han, J. (2011). Massively parallel concentration device for multiplexed immunoassays. Lab Chip. 11: 1351-1358 link
Cheow, L.F., Ko, S.H., Kim, S.J., Kang, K.H., and Han, J. (2010). Increasing the sensitivity of enzyme-linked immunosorbent assay using multiplexed electrokinetic concentrator. Anal. Chem. 82(8): 7086-7093 link
Yobas, L., Cheow, L.F., Tang, K.C., Yong, S.E., Ong, K.J., Wong, L., Teo, C.Y., Ji, H., Rafaeh, S., and Yu,C. (2009). A self-contained fully-enclosed microfluidic cartridge for lab on a chip. Biomed. Microdev. 11(6): 1279-1288 link
Luo, L.W., Teo, C.Y., Ong, W.L., Tang, K.C., Cheow, L.F., and Yobas, L. (2007). Rapid prototyping of microfluidic systems using a laser-patterned tape. J. Micromech. Microeng. 17:107-111 link
Schoch, R.B., Cheow, L.F., and Han, J. (2007). Electrical detection of fast reaction kinetics in nanochannels with an induced flow. Nano Letters. 7(12):3895-3900 link
Cheow, L.F., Yobas, L., and Kwong, D.L. (2007). Digital microfluidics: Droplet based logic. Appl. Phys. Lett. 90: 054107 link