Director

Dr. Kin Fong Lei is a Professor in Biomedical Engineering and Dean for International Affairs at Chang Gung University (CGU), Taiwan. Prior to joining CGU, he was a Lecturer at The Hong Kong Polytechnic University, Hong Kong (2007-2010). He received B.S. degree from National Tsing-Hua University, Taiwan (1998), and Ph.D. degree from The Chinese University of Hong Kong, Hong Kong (2005). In 2006, he was a post-doctoral fellow at the University of Western Ontario, Canada. Dr. Lei has made significant original contributions to research in bio-physics, bio-microfluidics, and bio-sensing. He has published over 100 academic articles and was invited to contribute in 8 book/book chapters. Dr. Lei has been listed in Career-long and 2020 World’s Top 2% Scientists and was selected as IEEE-NTC Distinguished Lecturer 2022.

Dr. Lei is a Fellow of the Royal Society of Chemistry (RSC), Fellow of the Institute of Physics (IOP), Senior Member of the Institute of Electrical and Electronics Engineers (IEEE), and Member of the American Society of Mechanical Engineers (ASME). He serves as a Chair of IEEE-EMBS Technical Committee on Bionanotechnology and BioMEMS (BNM) (2020-2021) and Chair of IEEE-NTC Technical Committee on Nanorobotics and Nanomanufacturing (2021-2022). He was Associate Editor at EMBS Conference Editorial Board in 2020. He also served as an organizing committee member for many IEEE conferences for MEMS/microfluidics researchers. Dr. Lei is an Associate Editor for IEEE Access and IEEE Transactions on NanoBioscience, and Editorial Board Member for Scientific Reports.  

Research Interests:

MEMS; Microfluidics; Bio-sensing.

Education:

Ph.D., The Chinese University of Hong Kong, 2005 

M.Phil., The Chinese University of Hong Kong, 2000 

B.Eng., National Tsing Hua University, 1998

Professional Experience :

Professor, Chang Gung University, 2018 - present

Visiting Scholar, Technical University of Munich, Germany, 2019 summer

Visiting Scholar, University of Colorado Denver, USA, 2016 summer

Visiting Scholar, The Chinese University of Hong Kong, Hong Kong, 2015 summer

Associate Professor, Chang Gung University, Taiwan, 2014-2018

Visiting Scholar, Academia Sinica Taiwan, 2013 summer

Assistant Professor, Chang Gung University, Taiwan, 2010-2014

Lecturer, The Hong Kong Polytechnic University, Hong Kong, 2007-2010

Associate Consultant, The Hong Kong Productivity Council, Hong Kong, 2006-2007

Postdoctoral Fellow, University of Western Ontario, Canada, 2006

Editorial Services :

Associate Editor, IEEE Transactions on NanoBioscience (served from 2020 to present)

Associate Editor, EMBS section, IEEE Access (served from 2019 to present)

Editorial Board Member, Micro and Nanosystems (served from 2018 to present)

Editorial Board Member, Scientific Reports (served from 2014 to present)

Research Projects:

1. PI, Analysis of cell proliferation and protein expression of multilayered artificial blood vessel in microfluidic channel under physcial and chemical stimulation, Ministry of Science and Technoloy, Taiwan MOST 111-2221-E-182-006-MY3, TW$4,206,000, 1 Aug 2022 - 31 July 2025.

2. PI, Investigation of cancer cell stemness based on cellular electrical impedance properties in a microfluidic platfrom, Ministry of Science and Technology, Taiwan, MOST110-2221-E-182-029, TW$1,226,000, Aug 1, 2021 – July 31, 2022.

3. PI, Investigation of the stem-like property of cancer cells co-culturing with macrophages under continuous microfluidic flow environment, Chang Gung Memorial Hospital, Taiwan, CMRPD2L0061, TW$2,060,866, Aug 1, 2021 – July 31, 2023.

4. PI, Investigation of the electrical properties of cancer stem cells using a dielectrophoretic and impedimetric hybrid microfluidic platform, Chang Gung Memorial Hospital, Taiwan, CMRPD2L0011, TW$2,812,929, Feb 1, 2021 – Jan 31, 2024.

5. PI, Cancer metastatic potential evaluated by biophysical properties of CTC based on automatically impedimetric monitoring of 3D cell invasion in a microfluidic system, Ministry of Science and Technology, Taiwan, MOST108-2221-E-182-058, TW$926,000, 1 Aug 2019 - 31 July 2020.

6. PI, Development of a 3D wound healing assay and impedimetric monitoring of cell invasion process in a microfluidic system, Chang Gung Memorial Hospital, CMRPD2J0061, TW$2,344,315, 1 Aug 2019 - 31 July 2021.

7. PI, Development of a nanofiber/polymer composited microfluidic system and demonstration on impedimetric monitoring of cell proliferation, chemosensitivity, hypoxia, migration, and multi-cellular co-culture in 3D environment, Ministry of Science and Technology, Taiwan, MOST107-2221-E-182-053-MY3, TW$3,160,000, 1 Aug 2018 - 31 July 2021.

8. PI, Development of a microfluidic system for the investigation of the inhibition mechanisms of tumor spheroids under the combined stimulation of anti-cancer drug and electric field, Chang Gung Memorial Hospital, CMRPD2H0021, TW$3,606,198, 1 Feb 2018 - 31 Jan 2021.

9. PI, Investigation of the inhibiting effect of co-culturing cancer cells and fibroblast cells under the application of alternating electric field in a microfluidic device, Chang Gung Memorial Hospital, CMRPD2G0171, TW$2,281,715, 1 Aug 2017 - 31 July 2019.

10. PI, Quantification of the formation process and the chemosensitivity of cancer cell colonies suspending in hydrogel during culture course, Chang Gung Memorial Hospital, CMRPD2F0031, TW$2,094,315, 1 Feb 2016 - 31 Jan 2018.

11. PI, Development of a paper-based 3D co-culture microfluidic system for real-time and non-invasive impedimetric monitoring of cell-cell interaction under various cytokine conditions, Ministry of Science and Technology, MOST104-2221-E-182-014-MY3, TW$2,721,000, 1 Aug 2015 - 31 July 2018.

12. PI, A paper-based cell culture and subsequent immunoassay microfluidic system for the investigation of cancer cell phosphorylation and signaling pathway, Chang Gung Memorial Hospital, CMRPD3E0101, TW$2,102,043, 1 Jan 2015 - 31 Dec 2016.

13. Co-PI, Real-time analysis and optimizing environment for human tenocyte proliferation, Ministry of Science and Technology, MOST103-2218-E-182A-003, TW$640,000, 1 Oct 2014 - 31 July 2015.

14. Co-PI, Real-time analysis of tissue-specific cellular proliferation and optimization in platelet-rich-plasma (PRP) prolotherapy, Chang Gung Memorial Hospital, CMRPG5D0171, TW$1,077,555, 1 Aug 2014 - 31 July 2015.

15. PI, Development of a microfluidic system incorporating with material regulation property for on-chip impedimetric monitoring cancer stem cell selection and anti-cancer drug screening, Ministry of Science and Technology, MOST103-2221-E-182-004-MY3, TW$2,902,000, 1 Aug 2014 - 31 July 2017.

16. PI, Label-free immunoassay quantitatively detected by paper-based microfluidic carbon nanotube sensing device, Chang Gung Memorial Hospital, CMRPD2D0021, TW$762,755, 1 Jan 2014 - 31 Dec 2014.

17. PI, Development of a microfluidic automatic immunoassay system and demonstration of rapid influenza screening, Chang Gung Memorial Hospital, CMRPD2C0141, TW$1,934,315, 1 Oct 2013 - 30 Sept 2015.

18. PI, Development of a microfluidic perfusion 3D cell culture and measurement biochip for continuous monitoring 3D cellular dynamic response under varied culture conditions, National Science Council, NSC101-2221-E-182-003-MY3, TW$2,538,000, 1 Aug 2012 - 31 July 2015.

19. PI, Investigation of blood coagulation time under various parameters, Chang Gung Memorial Hospital, CMRPD2B0011, TW$1,162,755, 1 Aug 2012 - 31 July 2013.

20. PI, Development of a thermo-pneumatic actuated immunoassay biochip, Chang Gung University, UERPD2B0121, TW$288,225, 1 Aug 2012 - 31 July 2013.

21. PI, Active hybridization and electrical detection in a DNA chip for portable diagnostics, National Science Council, NSC100-2221-E-182-022, TW$660,000, 1 Aug 2011 - 31 July 2012.

22. PI, A flexible capacitive normal and shear force sensor for pressure mapping application, Chang Gung University, UERPD2A0101, TW$608,900, 1 July 2011 - 30 June 2012.

23. PI, Development of a portable electrical immunoassay device, National Science Council, NSC99-2218-E-182-008, TW$681,000, 1 Dec 2010 - 31 Oct 2011.

Industrial Projects :

1. PI, Development of a quantitative evaluation system for spasticity in children with cerebral palsy, Mei Jing Biotechnology Ltd., Taiwan, TW$600,000, 15 July 2019 - 14 July 2020.

2. PI, DNA concentration bio-chip, Genvida (HK) Co Limited, Hong Kong, TW$500,000, 1 Mar 2018 - 31 Aug 2019.

3. PI, Development of a clinical screening platform for analyzing platelet-rich plasma (PRP), S.H. Medical Co. Ltd., Taiwan and Ministry of Science and Technology, Taiwan, MOST107-2622-E-182-004-CC3, TW$850,000, 1 Nov 2018 - 31 Oct 2019.

4. PI, Flexible capacitive pressure sensor, Mei Jing Biotechnology Ltd., Taiwan, TW$200,000, 15 July 2018 - 14 July 2019.

Selected Publications : (Full Publication list )

1. Chia-Hao Huang, Kin Fong Lei*, "Cell Marathon: Long-distance Cell Migration and Metastasis-associated Gene Analysis using a Folding Paper System", Lab Chip, 22:3827-3836, 2022.

2. Chun-Hao Huang, Kin Fong Lei*, “Quantitative Study of Tumor Angiogenesis in Three-dimensional Matrigel Barrier using Electric Impedance Measurement Technique”, Sensors and Actuators: B. Chemical, 370:132458, 2022.

3. Chia-Hao Huang, Kowit-Yu Chong, Kin Fong Lei*, “Analysis of the Internal Hypoxic Environment in Solid Tumor Tissue Using a Folding Paper System”, ACS Applied Materials & Interfaces, 13:33885-33893, 2021.

4. Chun-Hao Huang, Kin Fong Lei, "Impedimetric Quantification of Migration Speed of Cancer Cells Migrating along a Matrigel-filled Microchannel", Analytica Chimica Acta, 1121:67-73, 2020.

5. Andrew Goh, Chun-Chih Yeh, Kin Fong Lei, "Visualization and Quantification of 3D Tumor Cell Migration under Extracellular Stimulation", ACS Applied Bio Materials, 3:1506-1513, 2020.

6. Kin Fong Lei, Wun-Wu Ji, Andrew Goh, Chun-Hao Huang, Ming-Yih Lee, "Investigation of Uniform Sized Multicellular Spheroids Raised by Microwell Arrays after the Combined Treatment of Electric Field and Anti-cancer Drug", Biomedical Microdevices, 21:94, 2019.

7. Kin Fong Lei, Andrew Goh, Chun-Hao Huang, "Paper/polymer Composited Microfluidic Platform for Screening Cell Viability and Protein Expression under a Chemical Gradient Environment", Talanta, 205:120124, 2019.

8. Chun-Hao Huang, Kin Fong Lei, Ngan-Ming Tsang, "Apoptosis and Cell Cycle Arrest of Hepatocellular Carcinoma Spheroids Treated by an Alternating Electric Field", Biotechnology Progress, 35:e2787, 2019.

9. Kin Fong Lei, Shao-Chieh Hsieh, Andrew Goh, Rei-Lin Kuo, Ngan-Ming Tsang, "Proliferation Arrest, Selectivity, and Chemosensitivity Enhancement of Cancer Cells Treated by a Low-intensity Alternating Electric Field", Biomedical Microdevices, 20:90, 2018.

10. Chun-Hao Huang, Kin Fong Lei, Ngan-Ming Tsang, "Dissociated Effect and Chemosensitive Enhancement of Tumor Spheroids Influenced by an Electric Field in a Microdevice", Biomedical Microdevices, 20:70, 2018.

11. Yung-Chiang Liu, I-Chi Lee, Kin Fong Lei, "Toward the Development of an Artificial Brain on a Micropatterned and Material-regulated Biochip by Guiding and Promoting the Differentiation and Neurite Outgrowth of Neural Stem/Progenitor Cells", ACS Applied Materials & Interfaces, 10:5269-5277, 2018.

12. Kin Fong Lei, Tai-Kun Liu, Ngan-Ming Tsang, "Towards a High Throughput Impedimetric Screening of Chemosensitivity of Cancer Cells Suspended in Hydrogel and Cultured in a Paper Substrate", Biosensors and Bioelectronics, 100:355-360, 2018.

13. Kin Fong Lei, Chih-Hsuan Chang, Ming-Jie Chen, “Paper/PMMA Hybrid 3D Cell Culture Microfluidic Platform for the Study of Cellular Crosstalk”, ACS Applied Materials & Interfaces, 9:13092-13101, 2017.

14. Kin Fong Lei, Chich-Hao Kao, Ngan-Ming Tsang*, “High Throughput and Automatic Colony Formation Assay based on Impedance Measurement Technique”, Analytical and Bioanalytical Chemistry, 409:3271-3277, 2017.

15. Kin Fong Lei, Bo-Yuan Lin, Ngan-Ming Tsang, “Real-time and Label-free Impedimetric Analysis of the Formation and Drug Testing of Tumor Spheroids Formed via the Liquid Overlay Technique”, RSC Advances, 7:13939-13946, 2017.

16. Chih-Hao Chiu, Kin Fong Lei, Wen-Ling Yeh, "Development of a Co-culture Device for the Study of Human Tenocytes in Response to the Combined Stimulation of Electric Field and Platelet Rich Plasma (PRP)", Biomedical Microdevices, 19:69, 2017.

17. Chih-Hao Chiu, Jun-Liang Liu, Chih-Hsuan Chang, Kin Fong Lei, Alvin Chao-Yu Chen, “Investigation of Osteogenic Activity of Primary Rabbit Periosteal Cells Stimulated by Multi-axial Tensile Strain”, Biomedical Microdevices, 19:13, 2017.

18. Chia-Hao Huang, Kin Fong Lei, Ngan-Ming Tsang, "Paper-based Microreactor Array for Rapid Screening of Cell Signaling Cascades", Lab on a Chip, 16:2911-2920, 2016.

19. Kin Fong Lei, Chia-Hao Huang, Ngan-Ming Tsang, "Impedimetric Quantification of Cells Encapsulated in Hydrogel Cultured in a Paper-based Microchamber", Talanta, 147:628-633, 2016.

20. Kin Fong Lei, Zong-Ming Wu, Chia-Hao Huang, "Impedimetric Quantification of the Formation Process and the Chemosensitivity of Cancer Cell Colonies Suspended in 3D Environment", Biosensors and Bioelectronics 74:878-885, 2015.

21. Lu Liu, Xia Xiao Kin Fong Lei, Chia-Hao Huang, "Quantitative Impedimetric Monitoring of Cell Migration under the Stimulation of Cytokine or Anti-cancer Drug in a Microfluidic Chip", Biomicrofluidics 9:034109, 2015.

22. Kin Fong Lei, Chia-Hao Huang, Rei-Lin Kuo, Cheng-Kai Chang, Kuan-Fu Chen, Kuo-Chien Tsao, Ngan-Ming Tsang, "Paper-based Enzyme-free Immunoassay for Rapid Detection and Subtyping of Influenza A H1N1 and H3N2 Viruses", Analytica Chimica Acta, 883:37-44, 2015.

23. Kin Fong Lei, Yun-Hsiang Wang, Huai-Yi Chen, Jia-Hong Sun, Ji-Yen Cheng, "Electrokinetic Acceleration of DNA Hybridization in Microsystems", Talanta, 138:149-154, 2015.

24. Kin Fong Lei, Shih-I Yang, Shiao-Wen Tsai, Hsiao-Ting Hsu, "Paper-based Microfluidic Sensing Device for Label-free Immunoassay Demonstrated by Biotin-Avidin Binding Interaction", Talanta, 134:264-270, 2015.

25. Kin Fong Lei, Chia-Hao Huang, "Paper-based Microreactor Integrating Cell Culture and Subsequent Immunoassay for the Investigation of Cellular Phosphorylation", ACS Applied Materials & Interfaces, 6:22423-22429, 2014.

26. Kin Fong Lei, I-Chi Lee, Yung-Chiang Liu, Yu-Chieh Wu, "Successful Differentiation of Neural Stem/Progenitor Cells Cultured on Electrically Adjustable Indium Tin Oxide (ITO) Surface", Langmuir, 30:14241-14249, 2014.

27. Kin Fong Lei, Min-Hsien Wu, Che-Wei Hsu, Yi-Dao Chen, "Real-time and Non-invasive Impedimetric Monitoring of Cell Proliferation and Chemosensitivity in a Perfusion 3D Cell Culture Microfluidic Chip", Biosensors and Bioelectronics, 51:16-21, 2014.

28. Kin Fong Lei, Kuan-Hao Chen, Yu-Chen Chang, "Protein Binding Reaction Enhanced by Bi-directional Flow Driven by on-chip Thermopneumatic Actuator", Biomedical Microdevices, 16:325-332, 2014.

29. Yen-Heng Lin, Chia-Chu Wang, Kin Fong Lei, "Bubble-driven Mixer Integrated with a Microfluidic Bead-based ELISA for Rapid Bladder Cancer Biomarker Detection", Biomedical Microdevices, 16:199-207, 2014.

30. Kin Fong Lei, Kuan-Hao Chen, Po-Hsiang Tsui, Ngan-Ming Tsang, "Real-time Electrical Impedimetric Monitoring of Blood Coagulation Process under Temperature and Hematocrit Variations Conducted in a Microfluidic Chip", PLoS ONE, 8:e76243, 2013.

31. Kin Fong Lei, Min-Hsien Wu, Pei-You Liao, Yan Ming Chen, Tung-Ming Pan, "Development of a Micro-scale Perfusion 3D Cell Cultrue Biochip with an Incorporated Electrical Impedance Measurement Scheme for the Quantification of Cell Number in a Cell Culture Construct", Microfluidics and Nanofluidics, 12:117-125, 2012.

32. Kin Fong Lei and Yoki K.C. Butt, "Colorimetric Immunoassay Chip based on Gold Nanoparticles and Gold Enhancement", Microfluidics and Nanofluidics, 8:131-137, 2010.

33. Kin Fong Lei, Han Cheng, Kit Ying Choy, and Larry M.C. Chow, "Electrokinetic DNA Concentration in Micro System", Sensors and Actuators A: Physcial, 156:381-387, 2009.

34. Kin Fong Lei, Syed Ahsan, Nasser Budraa, Wen J. Li and John D. Mai, "Microwave Bonding of Polymer-based Substrates for Potential Encapsulated Micro/Nano Fluidic Device Fabrication", Sensors and Actuators A:Physcial, 114:340-346, 2004.