lambda quiz

1)

Restriction enzymes are enzymes isolated from?

Bacteria

Humans

Viruses

Mice

2)

What is the normal role of restriction enzymes in bacterial cells?

3)

Which type of restriction endonuclease cuts the DNA within the recognition site?

Type I

Type III

Type II

All the above

4)

5)

Which enzymes recognize and cleave specific 4 to 8 base pair sequences?

Ligases

Helicases

Restriction endonucleases

Polymerases

Sticky ends are the result of

6)

If a linear piece of DNA has two sites for BamHI restriction enzyme, into how many fragments will that restriction enzyme cut the DNA?

3

2

4

1

7)

8)

Star activity of the Restriction Enzyme is due to

DNA sequence that restriction enzymes cut are mostly:

9)

If a circular piece of DNA has four HindIII sites, into how many fragments will the DNA be cut?

5

3

4

6

10)

Bacterial cells protect their own DNA from degradation by restriction endonucleases