LIPID METABOLISM (SELECTED PUBLICATIONS)

Tang Yi Chien and Das, A.* Role of Omega-3 and Omega-6 Endocannabinoids  in Cardiopulmonary Pharmacology, Advances in Pharmacology Volume 97, Book Chapter. 2023

Arnold, W.,  Carnevale, L., Xie, Z., Baylon, J., Tajkhorshid, E., Hu, H., Das, A.*, "Anti-inflammatory dopamine- and serotonin-based endocannabinoid epoxides reciprocally regulate cannabinoid receptors and the TRPV1 channel " Nature Communications, 2021, https://www.nature.com/articles/s41467-021-20946-6

Das, A.*, Arnold, W.*, Weigle, A.*, Kim, J., Carnevale, L.,  and Huff, H., "CYP2J2 Molecular Recognition: A New Axis for Therapeutic Design", Pharmacology and Therapeutics, 2020,  Jun 11;107601. doi: 10.1016/j.pharmthera.2020.107601.

Watson, J, Kim, J. and Das, A* "Emerging class of omega-3 fatty acid endocannabinoids & their derivatives", Prostaglandins and Other Lipid Mediators (POLM), 2019, 11, 143

Roy, J., Watson, J., Hong, I., Fan, T., and Das, A*, "Anti-tumorigenic properties of omega-3 endocannabinoid epoxides" J. Med. Chem, 2018, 61, 5569

 McDougle, D.R., Watson, J., Abdeen,A., Adili, R., Caputo, M., Krapf, J., Johnson, R., Kilian, K., Holinstat, M., Das, A*, "Anti-inflammatory Omega-3 Endocannabinoid Epoxides", Proceedings of National Academy of Sciences (PNAS) (Direct Submission, Track II), 2017 https://doi.org/10.1073/pnas.1610325114

MINOR CANNABINOID METABOLISM (SELECTED PUBLICATIONS)

Pritam Roy, ‡, Jonathan Maturano†,a,b, Hale Hasdemirc, Angel Lopez‡, Fengyun Xud, , Judith Hellmand, Emad Tajkhorshidb,c, David Sarlah*,a,b and Aditi Das*, ‡Elucidating the Mechanism of Metabolism of Cannabichromene (CBC) by Human Cytochrome P450s. 2024, ACS- Journal of Natural Products, https://pubs.acs.org/doi/10.1021/acs.jnatprod.3c00336

Roy, P*., Dennis, D*., Eschbach, M., Anand, D., Hellman, J. Sarlah, D. * and Das, A*., "Metabolism of Cannabigerol by Human Liver Cytochrome P450s leading to bioactive CBG metabolites." 2022,  ACS-Biochemistry

Dennis, D., Anand, D., .... ., Das, A*, Sarlah, D*, "Synthesis of Cannabimovone and Cannabifuran Class of Minor cannabinoids and Their  Anti-inflammatory Activity," 2022, Journal of Organic Chemistry, https://pubs.acs.org/doi/10.1021/acs.joc.2c00336

Dutta,S., Selvam,B., Das, A. and Shukla, D*., Mechanistic origin of partial agonism of tetrahydrocannabinol for cannabinoid receptors  2022, Journal of Biological Chemistry, DOI:https://doi.org/10.1016/j.jbc.2022.101764

64. Cannabinoids

Sangiamo, D.T,  Weingarten, M.J, Choi, C.Y., Das, A. and  Liang, Nu-Chu*, "Consumption of dronabinol altered daily feeding patterns and affected alcohol-related changes in frontal endocannabinoid levels in male rats", 2023, Behavioral Brain Research, ASAP

63. Louie, A*., Kim, J*., Soto-Diaz, K., Dibaeinia, P., Koito, H., Sinha, S., Nowak, R. Das, A*., Steelman, A*.,“Influenza A virus infection disrupts oligodendrocyte homeostasis and alters the myelin lipidome in the adult mouse". 2023, Journal of Neuroinflammation,  Accepted (impact factor:9.5)

62. Cannabinoids

Torrens, A., Ruiz, Christina., Roy, P.,  Grimes, D., Ahmed, F.,  Huestis, M.,  Mahler, S., Das, A., Piomelli, D.* "Nasal Accumulation and Metabolism of 9-Tetrahydrocannabinol Following Aerosol Administration in Adolescent Rats" 2023, Pharmacological Research, doi: 10.1016/j.phrs.2022.106600.

2022

61. Cannabinoids and CYP Biophysics

Roy, P*., Dennis, D*., Eschbach, M., Anand, D., Hellman, J. Sarlah, D. * and Das, A*., "Metabolism of Cannabigerol by Human Liver Cytochrome P450s leading to bioactive CBG metabolites." 2022,  Biochemistry, 2398

60. Biophysics

Ulusan,S., Sheraj, I., Stehling,S., Ivanov, I., Das, A., Kuhn, H.,  Banerjee, S*. Structural and functional evaluation mammalian and plant lipoxygenases upon association with nanodics as membrane mimetics. 2022, Biophysical Chemistry, DOI: 10.1016/j.bpc.2022.106855

59. Cannabinoids

Dennis DG, Anand SD, Lopez AJ, Petrovčič J, Das A*, Sarlah D*, "Synthesis of Cannabimovone and Cannabifuran Class of Minor cannabinoids and Their  Anti-inflammatory Activity," 2022, Journal of Organic Chemistry, 6075. 

58. Xie , Feng., Cai, McCarthy, Wang, Zhao, Zang, Yuan, Hu, Li, Liu, Das, A., England, S. and Hu, H.*, "Activation of nociceptor by estrogen metabolites: a peripheral mechanism underlying sex differences in pain perception?" 2022, Journal of Clinical Investigation Insight, https://insight.jci.org/articles/view/149107

2021

36. Arnold, W., Weigle, A. and Das, A*, "Crosstalk of Endocannabinoids and Cannabinoids metabolism is mediated by CYP2J2 epoxygenase" Journal of Inorganic Chemistry,  2018,  184, 88

2017

35. Arnold, W., Baylon, J., Tajkhorshid, E.* and Das, A*. "Arachidonic Acid Metabolism by Human Cardiovascular CYP2J2 Is Modulated by Doxorubicin." Biochemistry, 2017, 56 (51), pp 6700–6712

F1000 Prime Recommended!!

2016

31.  Arnold, W., Baylon, J, Tajkorshid, E.* and Das, A.*,"Asymmetric Binding and Metabolism of Polyunsaturated Fatty Acids (PUFAs) by CYP2J2 Epoxygenase" Biochemistry. 2016, 55(50):6969-6980. 

30. Roy, J. Adili, Raymond, Kulmacz, R., Holistat, M., and Das, A*,"Development of Poly Unsaturated Fatty Acid Derivatives of Aspirin for Inhibition of Platelet Function", Journal of Pharmacology and Experimental Therapeutics, 2016 Oct;359(1):134-41.

29. Bradley*, WB., Rouck*, J.E., Kambalyal, A., Das, A*. and Parayil, A*., "Orthogonal assays uncover enzyme promiscuity and chemical isomerization and remove ambiguity surrounding key P450 mediated oxygenation in Taxol biosynthesis". ACS-Chemical Biology, 2016, 11 (5), 1445–1451.

28. Plucinski, L., Gartia, M., Arnold, W., Ameen, A., Chang, T., Hsiao, A., Liu, G.*, Das, A.*, "Substrate Binding to CYP2J2- Nanodiscs Detected by Nanoplasmonic Lygurcus Cup Arrays" Biosens Bioelectron., 2016, 337-46. 

d0i: 1016/j.bios.2015.07.041 

27. Lim, SJ, McDougle, D., Das, A* and Smith, A.*, "Lipoprotein Nanoplatelets: Fluorescent, Zwitterionic Probes for Molecular and Cellular Imaging." J. Am. Chem. Soc., 2016, 138 (1), pp 64–67

24.  Zelasko, S, Meling, D. and Das A*. "A critical residue that controls the isomerization of prostaglandin in thromboxane synthase", Biophysical Chemistry, 2015, 34-40.

23. Wilcox, K., Das, A., Velasco, P., Sligar S.G. and Klein, W. "Nanoscale synaptic membrane mimetic allows unbiased high throughput screen that targets binding sites for Alzheimer’s-associated Aβ oligomers" 2015, PLOS ONE In Press

22. Zelasko, S., Arnold, W., Das, A* " Endocannabinoid Metabolism by Cytochrome P450 Monooxygenases.", Prostaglandins and Other Lipid Mediators. 2015.

(News: Most downloaded article is at POLM)

18. Srivarma, S., Mularcyzk, C. Meling, D. and Das, A*. "Substrate binding thermodynamics and kinetics to thromboxane synthase in model lipid bilayers", ChemBioChem. 2014, 15, 892

***************(Before start of faculty position)********************

15. Marty, M., Das, A., Sligar, S.G. “Ultra-thin layer MALDI mass spectrometry of membrane proteins in nanodiscs”, Analytical Bioanalytical Chemistry, 2012, 402, 721.

14. Turk, S., Das, A., Sligar, S. G. and Dravid, V. “Nanomechanical Detection of Cholera Toxin Using Microcantilevers Functionalized with Ganglioside-Nanodiscs” Nanotechnology, 2010, 21, 435502.

13. Das, A., Wei, Y., Pelczer, I. and Hecht, M.H. “Binding of Small Molecules to Cavity Forming Mutants of a De Novo Designed Protein”, Protein Science, 2011, 20, 702–711.

(In News) Editor Highlight in Protein Science

(In News): Nanomechanical sensor can detect cholera. Nanowerk Spotlight

12. Das, A. and Sligar, S.G. “Modulation of the Cytochrome P450 Reductase Redox Potential by the Phospholipid Bilayer” Biochemistry, 2009, 48(51), 12104-12.

11. Das. A., Zhao, J. Van Duyne, R. and Sligar, S.G. “Screening of Type I and II Drug Binding to Human Cytochrome P450-3A4 in Nanodiscs by Localized Surface Plasmon Resonance Spectroscopy”, Anal. Chem., 2009, 81 (10), 3754–3759.

10. Li, H., Das, A., Sibhatu, H., Jamal, J., Sligar, S. and Poulos, T.L. “Exploring the Electron Transfer Properties of Neuronal Nitric Oxide Synthase by Reversal of the FMN Redox Potential” J. Biol. Chem. 2008, 283 (50), 34762-34772.

9. Zhao,J., Das, A. Sligar, S.G. and Van Duyne, R. “Resonance Localized Surface Plasmon Spectroscopy: Sensing Substrate & Inhibitor Binding to Cytochrome P450” J. Phys. Chem. C.2008, 112 (34), 13084-13088.

8. Gruia,F., Ionascu,D. , Kubo, M. Ye, X., Dawson, J., Osborne, R.L., Sligar, S. G., Denisov, I., Das, A. Poulos, T. L., Terner, J. and Champion, P.M. “Low Frequency Dynamics of Caldariomyces fumago Chloroperoxidase Probed by Femtosecond Coherence Spectroscopy” Biochemistry 2008, 47(18), 5156-5167.

7. Das. A., Grinkova, Y. and Sligar, S.G. “Redox Potential Control by Drug Binding to Cytochrome P450 3A4.” J. Am. Chem. Soc. 2007, 129 (45), 13778-13779

6. Das, A. and Hecht, M. H. “Peroxidase Activity of De Novo Heme Proteins Immobilized on Electrodes.” J. Inorg. Biochem. 2007, 101 (11-12), 1820-1826  

5. Kimmich, N., Das. A., Sevrioukova, I, Meharenna, Y., Sligar, S.G. and Poulos, T. L. “Electron Transfer between P450cin and its FMN-Containing Redox Partner” J. Biol. Chem., 2007, 282 (37), 27006-27011

4. Zhao*, J., Das, A.*, Zhang, X, Schatz, G., Sligar, S., Van Duyne, R. “Resonance Surface Plasmon Spectroscopy: Low Molecular Weight Substrate Binding to Cytochrome P450” J. Am. Chem. Soc. 2006, 128 (34), 11004-11005. (* Co-first authors)

(In News): (1) Enhanced LSPR detects binding of small molecules. Anal. Chem., 2006, 78(21), 7356. (2) Kirrill’s Café paper of the month. August 2006

3. Das, A., Trammell, S. A. and Hecht, M. H. “Electrochemical and Binding Studies of an immobilized De Novo Heme Protein” Biophy. Chem. 2006, 123 (2-3), 102-112.

2. Hu, Y., Das, A., Hecht, M. H. and Scoles, G. “Nanografting De Novo Proteins onto gold surface” Langmuir 2005, 21(20), 9103-9109.

1. Hecht, M.H., Das, A., Go, A., Bradley, L. and Wei, Y. (2004) “De Novo Proteins from Designed Combinatorial Libraries.” Protein Science 2004, 13(7), 1711–1723.

THESIS 

Das, A.  “Electrochemical and Functional Studies of De Novo Alpha Helical Protein from a Designed Combinatorial Library.” Ph.D Thesis Princeton University November 2005

Das, A. Model Chemistry of Aerobic Acetylene Hydratase Enzymes. M.Sc. Thesis: I.I.T (Kanpur) 1998

BOOK CHAPTER

Maroutsos, D., Huff, H., and Das, A*, "N-terminal modification to increase expression of Eukaryotic P450s in E.Coli - CYP5alpha Hydroxylase and CYP2J2 epoxygenase" Methods in Molecular Biology, Book Chapter. 

PATENTS

• Das, A. and Watson, J., “EPA endocannabinoid derivatives as Anti-inflammatory and Anti-pain drugs”, US Patent Application 2017-168-01

• Das, A. and Ray, J., “DHA endocannabinoid derivatives as Anti-inflammatory and Anti-pain drugs”, US Patent Application 2017-167-01.

• Smith, A., Lim, S.J., Das, A., “Lipoprotein Nanoplatelets:  Fluorescent, Zwitterionic Probes for Molecular and Cellular Imaging” US Patent Application 15/244,026

• Das, A. and Ray, J., “Aspirin derivatives of Omega-3 fatty acids”, Invention Disclosure. 

PAPERS IN GESTATION 

Endocannabinoid metabolism by Cytochrome P450s

• Tang, Yi-Chien and Das, A*, " Substrate selectivity in CYP2U1 in Nanodiscs"

• Kim and Das, A*, "Mechanistic understanding of the generation and metabolism of bioactive DHA based endocannabinoids in the central nervous system"

• EET receptors: Are we there yet? Manuscript in preparation, Review

• Role of Endocannabinoids and oxy-endocannabinoids in Cancer, Review

Cannabinoid metabolism by cytochrome P450s

• Kundu and Das, A*, "Metabolism of cannabinol by P450s, the active metabolites in sleep induction"

• Computational modeling and prediction of agonism and antagonism of cannabinoid receptor 1 and 2 by synthetic and naturally occurring cannabinoids, with Shukla Group.