2025
78. Tagne, A., Ahmed. F., Tran, A., Debbaneh, L., Perranoski, E., Sarlah, D., Das, A., Pabon, E., Copper, Z., and Piomelli, D., "Rapid and sensitive method for the quantification of cannabigerol and its application in mice". Submitted 2025
77. Liu,C., Smith, J.W., Argun, R., Bal, P., Liu, D., Li, J., Pan, P., Kollarigowda, R., Wang, H., Das, A., Schroeder, C., Dan, Krogstad, Braun, P.V., Statt, A., Chen, Q.,* Visualizing Nanoscale Heterogeneity in Responsive Polymer Hydrogel Networks, Submitted 2025
76. Tang, Yi-Chien (Tiffany), Li, Yupeng, Chapman, P. , Tajkhorshid E, Das, A*., "CYP2U1 and CYP2D6 Mediated Biotransformation of Sunitinib and Sorafenib Reveals Potential Anti-Cancer Metabolites"
2025, Submitted
75. Kundu, D., Franchini, L., Hasdemir, H.S., Lloyd, E., Maturano, J., Rabl, K., Denissioul, A., Schumacher, M., Sarlah, D., Hellman, J., Tajkhorshid, E., Orlandi, C., Das, A*, "Distinct Interactions of Cannabinol and Its Cytochrome P450-Generated Metabolites with Receptors and Sensory Neurons"
2025, Journal of Medicinal Chemistry. https://pubs.acs.org/doi/10.1021/acs.jmedchem.5c00938
74. Arnold, W.R., Jain, S., Sinha, V., Das, A*," The Hunt for the Putative Epoxyeicosatrienoic Acid Receptor" 2025, ACS Chemical Biology (Special Issue: Lipid and Lipidation) https://pubs.acs.org/doi/10.1021/acschembio.5c00047
Epoxyeicosatrienoic acids, or EETs, are signaling molecules formed by the metabolism of arachidonic acid by cytochrome P450 enzymes. They are well-known for their anti-inflammatory effects, their ability to lower blood pressure, and benefits to cardiovascular outcomes. Despite the wealth of data demonstrating their physiological benefits, the putative high-affinity receptor that mediates these effects is yet to be identified. In this review, we summarize the evidence supporting that the putative EET receptor exists.
73. Nils Helge Schebb*,# (NHS), ....... Das, A..................Valerie B. O’Donnell# (VBO) "Technical recommendations for liquid chromatography-mass spectrometry analysis of oxylipins." 2025 Science Signaling, 19, 997
2024
72. Li, J., Smith, J. K.Y. Huang, Wang, H., Das, A., Kong, H., and Chen, Q., "Towards Correlative Electron Microscopy Imaging for Proteins and Cells" 2024, Microscopy & Microanalysis " 2024, Microscopy and Microanalysis 30, Supplement_1: ozae044.807
71. Roy,P., Maturano, J., Hasdemir,H., Lopez, A., Xud, F. , Hellman,J., Tajkhorshid,E., Sarlah, D.* and Das,A.*, Elucidating the Mechanism of Metabolism of Cannabichromene (CBC) by Human Cytochrome P450s. 2024, ACS- Journal of Natural Products, https://pubs.acs.org/doi/10.1021/acs.jnatprod.3c00336
70. Smith, J., Carnevale, L., Das, A*, Chen, Q.*,"Electron Videography of a Lipid–Protein Tango",
2024, Science Advances doi: 10.1126/sciadv.adk0217.2024
69. Huff, H.C., §, Kim,J.S. and Das, A*.,"Real time changes in the expression of cyclooxygenase, lipoxygenase, and cytochrome P450s during inflammation"
2024, Prostaglandins Other Lipid Mediators, 106839.
Line graphs demonstrating relative expression over time of Non-LPS control (black) vs LPS (red) stimulation of inflammatory genes in macrophages.
2023
68. Kim,J., Soto-Diaz, K., Bingham, T., Steelman, A.J * and Das, A.* Role of Omega-3 Endocannabinoids in the Modulation of T-cell Activity in a Multiple Sclerosis EAE Model. 2023, Journal of Biological Chemistry., 102886.
67. Sangiamo, D.T, Weingarten, M.J, Choi, C.Y., Das, A. and Liang, Nu-Chu*, "Consumption of dronabinol altered daily feeding patterns and affected alcohol-related changes in frontal endocannabinoid levels in male rats", 2023, Behavioral Brain Research, ASAP
66. Louie, A*., Kim, J*., Soto-Diaz, K., Dibaeinia, P., Koito, H., Sinha, S., Nowak, R. Das, A*., Steelman, A*.,“Influenza A virus infection disrupts oligodendrocyte homeostasis and alters the myelin lipidome in the adult mouse". 2023, Journal of Neuroinflammation, Accepted (impact factor:9.5)
65. Torrens, A., Ruiz, Christina., Roy, P., Grimes, D., Ahmed, F., Huestis, M., Mahler, S., Das, A., Piomelli, D.* "Nasal Accumulation and Metabolism of 9-Tetrahydrocannabinol Following Aerosol Administration in Adolescent Rats" 2023, Pharmacological Research, doi: 10.1016/j.phrs.2022.106600.
64. Roy, P*., Dennis, D*., Eschbach, M., Anand, D., Hellman, J. Sarlah, D. * and Das, A*., "Metabolism of Cannabigerol by Human Liver Cytochrome P450s leading to bioactive CBG metabolites." 2022, Biochemistry, 2398
63. Ulusan,S., Sheraj, I., Stehling,S., Ivanov, I., Das, A., Kuhn, H., Banerjee, S*. Structural and functional evaluation mammalian and plant lipoxygenases upon association with nanodics as membrane mimetics. 2022, Biophysical Chemistry, DOI: 10.1016/j.bpc.2022.106855
62. Dennis DG, Anand SD, Lopez AJ, Petrovčič J, Das A*, Sarlah D*, "Synthesis of Cannabimovone and Cannabifuran Class of Minor cannabinoids and Their Anti-inflammatory Activity," 2022, Journal of Organic Chemistry, 6075.
61. Xie , Feng., Cai, McCarthy, Wang, Zhao, Zang, Yuan, Hu, Li, Liu, Das, A., England, S. and Hu, H.*, "Activation of nociceptor by estrogen metabolites: a peripheral mechanism underlying sex differences in pain perception?" 2022, Journal of Clinical Investigation Insight, https://insight.jci.org/articles/view/149107
60. Dutta,S., Selvam,B., Das, A. and Shukla, D*., Mechanistic origin of partial agonism of tetrahydrocannabinol for cannabinoid receptors 2022, Journal of Biological Chemistry, DOI:https://doi.org/10.1016/j.jbc.2022.101764
59. Torrens, A., Roy, P., Lin, L., Vu, C., Grimes, D., Inshishian, V., Montesinos, Ahmed, Mahler, S., Huestis, M., Das, Aditi, Piomelli, D*., "Comparative Pharmacokinetics of Δ9-Tetrahydrocannabinol in Adolescent and Adult Rats of Both Sexes." 2022, Cannabis and Cannabinoid Research https://doi.org/10.1089/can.2021.0205
58. Kim, J*., Arango, A*., Shah, S., Arnold, W., Tajkhorshid, E., Das, A*., "Anthracycline derivatives inhibit cardiac CYP2J2" J Inorg Biochem. 2022 doi: 10.1016/j.jinorgbio.2022.111722.
57. Huff, H., Vasan, A., Roy, P., Kaul, A., Tajkhorshid, E*., and Das, A*., "Differential Interactions of Selected Phytocannabinoids with Human CYP2D6 Polymorphisms", ACS-Biochemistry, 2021, 60, 2749.
56B. Arnold, W., Carnevale, L., Xie, Z., Baylon, J., Tajkhorshid, E., Hu, H., Das, A.*, "Anti-inflammatory dopamine- and serotonin-based endocannabinoid epoxides reciprocally regulate cannabinoid receptors and the TRPV1 channel " Nature Communications, 2021, https://www.nature.com/articles/s41467-021-20946-6
Science Daily: https://www.sciencedaily.com/releases/2021/02/210210091142.htm
Illinois: https://news.illinois.edu/view/6367/2092420617
Eureka Alert: https://www.eurekalert.org/pub_releases/2021-02/uoia-let020821.php
56A. Arnold, W., Carnevale, L., Xie, Z., Baylon, J., Tajkhorshid, E., Hu, H., Das, A.*, "Anti-inflammatory dopamine- and serotonin-based endocannabinoid epoxides reciprocally regulate cannabinoid receptors and the TRPV1 channel " BioRxiv, 2020. https://www.biorxiv.org/content/10.1101/2020.05.05.079624v1
55. Das, A.*, Arnold, W.*, Weigle, A.*, Kim, J., Carnevale, L., and Huff, H., "CYP2J2 Molecular Recognition: A New Axis for Therapeutic Design", Pharmacology and Therapeutics, 2020, 151-160.
54. Torrens,A., Vozella, V., Huff, H., McNeil, B., Ahmed, Ghidini, Mahler, Huestis, Das, A. and Daniele Piomelli. Comparative Pharmacokinetics of 9-Tetrahydrocannabinol in Adolescent and Adult Male Mice. Journal of Pharmacology and Experimental Therapeutics, 2020, doi: 10.1124/jpet.120.265892
53. Chen,A., Yeung, J., Szatkowski,A., Jackson, M., Freedman, C., Das, A., Holman, T., and Holinstat, M., "Omega-6 DPA and its 12-lipoxygenase oxidized lipids regulate platelet reactivity in a non-genomic PPARα-dependent manner" Blood Advances, 2020, 4522-4537.
52. Yadav, C. Reinhardt, H. Huff, M, Malkoswki, Das, A, Tajkhorshid, E., and J. Chan. "CoxFluor: An activity-based fluorescence probe for cyclooxygenase-2 (COX-2)", Angew Chem Int Ed Engl. 2020, doi: 10.1002/anie.201914845
51. Smith,J., Carnevale, L., Das, A*., and Qian Chen* "Real-time Electron Microscopy of Protein Nanodiscs in Graphene Liquid Cells", Microscopy and Microanalysis, 2019, 25 (S2), pp 1498-1499
50. Arnold, W., Meling, D., Zelasko, S. and Das, A*. "CYP2C8 polymorphisms causes disruption of the electron transfer between CYP and CPR", International Journal of Molecular Science, 2019, Accepted.
49. Li, Nader; Zhang, X. Ray,B., Kim, CY, Das, A., Witola, W.,"Novel lactate dehydrogenase inhibitors with in vivo efficacy against Cryptosporidium parvum" PLOS Pathogens, 2019, Accepted
48. Caputo,M., Radlowski, E., Lawson, M., Antonson, A., Watson, J., Matt, S., Leyshon, B., Das, A., and Johnson, R., "Herring Roe Oil Supplementation Alters Microglial Cell Gene Expression and Reduces Peripheral Inflammation After Immune Activation in a Neonatal Piglet Model", Brain, Behavior and Immunity, 2019, 30160.
47. Watson, J, Kim, J. and Das, A* "Emerging class of omega-3 fatty acid endocannabinoids & their derivatives", Prostaglandins and Other Lipid Mediators (POLM), 2019, 11, 143
46. Huff, H.,Maroutsos, D., and Das, A*,"Macromolecular Crowding and Lipid Composition Controls CYP2J2 activity. Protein Science, 2019, 28,928-940.
45. Roy, J., Fan, T., Payam, Sinha S., and Das, A*, "Global Analysis of Osteosarcoma Lipidomes Reveal Altered Lipid Profiles in Metastatic vs. Non-Metastatic Cells" Journal of Lipid Research, 2019, 60, 375-387
44. Nelson, N., Law, Weingarten,W., Carnevale, L., Das, A. Liang, Nu-Chu, "Subcutaneous or oral delta9-tetrahydrocannabinol administration in combination with moderate alcohol consumption differently alter ingestive behaviors in adolescent rats", Psychopharmacology, 2019, 236,671-684.
43. Carnevale, L., Arango, A., Arnold, W., Tajkhorshid, E*., and Das, A*,"Endocannabinoid Virodhamine Inhibits Cardiovascular CYP2J2 epoxygenase", Biochemistry, 2018, 57,6489-6499.
42. Roy, J., Watson, J., Hong, I., Fan, T., and Das, A*, "Anti-tumorigenic properties of omega-3 endocannabinoid epoxides" J. Med. Chem, 2018, 61, 5569
In News: https://news.illinois.edu/view/6367/670666
Current Altmetric Score: 217
Highlighted in https://acs.altmetric.com/details/43321817/news
41. Arnold, W., and Das, A*,"An Emerging Pathway of Doxorubicin Cardiotoxicity Mediated through CYP2J2, Biochemistry, 2018, 57, 2294
Gordon Hammes Scholar Award runner up.
In News: https://beckman.illinois.edu/news/2018/04/doxorubicin-and-cardiotoxicity
40. Roy, J. Pondenis, H., Fan T.*, Das, A*, "Comparative Proteomic Analysis of Metastatic and Non-metastatic Osteosarcoma of Human and Canine Origin" PLOS One, 2018 e0183930. doi: 10.1371/journal.pone.0183930.
39. Arnold, W., Weigle, A. and Das, A*, "Crosstalk of Endocannabinoids and Cannabinoids metabolism is mediated by CYP2J2 epoxygenase" Journal of Inorganic Chemistry, 2018, 184, 88
38. Arnold, W., Baylon, J., Tajkhorshid, E.* and Das, A*. "Arachidonic Acid Metabolism by Human Cardiovascular CYP2J2 Is Modulated by Doxorubicin." Biochemistry, 2017, 56 (51), pp 6700–6712
F1000 Prime Recommended!!
37. McDougle, D.R., Watson, J., Abdeen,A., Adili, R., Caputo, M., Krapf, J., Johnson, R., Kilian, K., Holinstat, M., Das, A*, "Anti-inflammatory Omega-3 Endocannabinoid Epoxides", PNAS (Direct Submission), 2017 https://doi.org/10.1073/pnas.1610325114
Highlighted in Forbes
Highlighted in Bioscience Technology
Highlighted in Nutrition Insight
Highlighted in New Medical Life Sciences
Highlighted in Eurekalert
36. Rouck, J.*, Krapf, J.* Roy, J. Huff, H. and Das, A.*, "Recent Advances in Nanodisc Technology for studying Membrane Proteins (2012-2017)". FEBS Letters 2017, Accepted
35. Rouck*, J.E., Bradley*, WB.,Kambalyal, A.,Parayil, A*. and Das, A*., "Expression and Purification of cytochrome P450 taxadiene 5alpha-hydroxylase in E.Coli", Protein Expression and Purification, Protein Expr Purif. 2017 Jan 18;132:60-67.
34. Arnold, W., Baylon, J, Tajkorshid, E.* and Das, A.*,"Asymmetric Binding and Metabolism of Polyunsaturated Fatty Acids (PUFAs) by CYP2J2 Epoxygenase" Biochemistry. 2016, 55(50):6969-6980.
33. Roy, J. Adili, Raymond, Kulmacz, R., Holistat, M., and Das, A*,"Development of Poly Unsaturated Fatty Acid Derivatives of Aspirin for Inhibition of Platelet Function", Journal of Pharmacology and Experimental Therapeutics, 2016 Oct;359(1):134-41.
32. Bradley*, WB., Rouck*, J.E., Kambalyal, A., Das, A*. and Parayil, A*., "Orthogonal assays uncover enzyme promiscuity and chemical isomerization and remove ambiguity surrounding key P450 mediated oxygenation in Taxol biosynthesis". ACS-Chemical Biology, 2016, 11 (5), 1445–1451.
31. Plucinski, L., Gartia, M., Arnold, W., Ameen, A., Chang, T., Hsiao, A., Liu, G.*, Das, A.*, "Substrate Binding to CYP2J2- Nanodiscs Detected by Nanoplasmonic Lygurcus Cup Arrays" Biosens Bioelectron., 2016, 337-46.
d0i: 1016/j.bios.2015.07.041
30. Lim, SJ, McDougle, D., Das, A* and Smith, A.*, "Lipoprotein Nanoplatelets: Fluorescent, Zwitterionic Probes for Molecular and Cellular Imaging." J. Am. Chem. Soc., 2016, 138 (1), pp 64–67
29. Roy, J. Pondenis, H., Fan T.*, Das, A*, "Direct Capture of Functional Proteins from Mammalian Plasma Membranes into Nanodiscs" Biochemistry, 2015, 6299–6302.
28. McDougle, D*., Baylon, J.* Meling D., Kambalyal, A., Tajkorshid, E.*, and Das, A* "Incorporation of Charged Residues in the CYP2J2 F-G Loop Disrupts CYP2J2-Lipid Bilayer Interactions" Biochimica et Biophysica Acta -Biomembranes, 2015, 2060-2470.
27. Zelasko, S, Meling, D. and Das A*. "A critical residue that controls the isomerization of prostaglandin in thromboxane synthase", Biophysical Chemistry, 2015, 34-40.
26. Wilcox, K., Das, A., Velasco, P., Sligar S.G. and Klein, W. "Nanoscale synaptic membrane mimetic allows unbiased high throughput screen that targets binding sites for Alzheimer’s-associated Aβ oligomers" 2015, PLOS ONE In Press
25. Zelasko, S., Arnold, W., Das, A* " Endocannabinoid Metabolism by Cytochrome P450 Monooxygenases.", Prostaglandins and Other Lipid Mediators. 2015.
(News: Most downloaded article is at POLM)
24. McDougle, D., Kambalayal, A., Meling, D. and Das, A*. "Endocannabinoids - Anandamide and 2- Arachidonoylglycerol are Substrates for Human CYP2J2 Epoxygenase" Journal of Pharmacology and Experimental Therapeutics, 2014, 616-27.
23. Meling, D., McDougle, D. and Das, A.*,"CYP2J2 epoxygenase membrane anchor plays an important role in facilitating electron transfer from CPR." Journal of Inorganic Biochemistry 2014, 47-53.
22. Orlando, B., McDougle, D., Lucido, M., Eng, E., Stokes, D., Das, A*., and Malkowski, M*. "Cyclooxygenase-2 Catalysis and Inhibition in Lipid Bilayer Nanodiscs" Archives of Biochemistry and Biophysics 2014, 546, 33-40.
21. Srivarma, S., Mularcyzk, C. Meling, D. and Das, A*. "Substrate binding thermodynamics and kinetics to thromboxane synthase in model lipid bilayers", ChemBioChem. 2014, 15, 892
20. Zelasko S., Palaria, A., and Das, A* "Modifications to produce high-level expression of mammalian cytochrome P450s in recombinant expression systems" Protein Expression and Purification, 2013,92,77-87
19. McDougle, D*., Palaria, A.*Magnetta E., Meling, D. and Das, A*. "Functional Studies of N-terminally modified CYP2J2 epoxygenase in Model Lipid Bilayers", Protein Science, 2013, 22,964 (Amrita Palaria and Daniel McDougle are equal authors)
BOOK CHAPTERS
18. Tang Yi ChienG and Das, A.* (2023) Role of Omega-3 and Omega-6 Endocannabinoids in Cardiopulmonary Pharmacology, Advances in Pharmacology Volume 97, Book Chapter. 375-422. doi: 10.1016/bs.apha.2023.02.003. (Special invited book chapter)
17. Carnevale, L., and Das, A*, (2019) "Novel Anti-inflammatory and Vasodilatory ω-3 Endocannabinoid Epoxide Regioisomers", Book Chapter, Bioactive Lipids in Cancer, Inflammation and Related Diseases. Advances in Experimental Medicine and Biology (AEMB). 1161:219-232. doi: 10.1007/978-3-030-21735-8_17. (Special invited book chapter)
16. Maroutsos,D., Huff, H., Das, A*., (2019) "Bacterial Expression of Membrane-Associated Cytochrome P450s and Their Activity Assay in Nanodiscs, Book Chapter, Microbial Metabolic Engineering. Methods in Molecular Bioligy 1927: 47-72. doi: 10.1007/978-1-4939-9142-6_5. (Special invited book chapter).
15. Marty, M., Das, A., Sligar, S.G. “Ultra-thin layer MALDI mass spectrometry of membrane proteins in nanodiscs”, Analytical Bioanalytical Chemistry, 2012, 402, 721.
14. Turk, S., Das, A., Sligar, S. G. and Dravid, V. “Nanomechanical Detection of Cholera Toxin Using Microcantilevers Functionalized with Ganglioside-Nanodiscs” Nanotechnology, 2010, 21, 435502.
13. Das, A., Wei, Y., Pelczer, I. and Hecht, M.H. “Binding of Small Molecules to Cavity Forming Mutants of a De Novo Designed Protein”, Protein Science, 2011, 20, 702–711.
(In News) Editor Highlight in Protein Science
(In News): Nanomechanical sensor can detect cholera. Nanowerk Spotlight
12. Das, A. and Sligar, S.G. “Modulation of the Cytochrome P450 Reductase Redox Potential by the Phospholipid Bilayer” Biochemistry, 2009, 48(51), 12104-12.
11. Das. A., Zhao, J. Van Duyne, R. and Sligar, S.G. “Screening of Type I and II Drug Binding to Human Cytochrome P450-3A4 in Nanodiscs by Localized Surface Plasmon Resonance Spectroscopy”, Anal. Chem., 2009, 81 (10), 3754–3759.
10. Li, H., Das, A., Sibhatu, H., Jamal, J., Sligar, S. and Poulos, T.L. “Exploring the Electron Transfer Properties of Neuronal Nitric Oxide Synthase by Reversal of the FMN Redox Potential” J. Biol. Chem. 2008, 283 (50), 34762-34772.
9. Zhao,J., Das, A. Sligar, S.G. and Van Duyne, R. “Resonance Localized Surface Plasmon Spectroscopy: Sensing Substrate & Inhibitor Binding to Cytochrome P450” J. Phys. Chem. C.2008, 112 (34), 13084-13088.
8. Gruia,F., Ionascu,D. , Kubo, M. Ye, X., Dawson, J., Osborne, R.L., Sligar, S. G., Denisov, I., Das, A. Poulos, T. L., Terner, J. and Champion, P.M. “Low Frequency Dynamics of Caldariomyces fumago Chloroperoxidase Probed by Femtosecond Coherence Spectroscopy” Biochemistry 2008, 47(18), 5156-5167.
7. Das. A., Grinkova, Y. and Sligar, S.G. “Redox Potential Control by Drug Binding to Cytochrome P450 3A4.” J. Am. Chem. Soc. 2007, 129 (45), 13778-13779
6. Das, A. and Hecht, M. H. “Peroxidase Activity of De Novo Heme Proteins Immobilized on Electrodes.” J. Inorg. Biochem. 2007, 101 (11-12), 1820-1826
5. Kimmich, N., Das. A., Sevrioukova, I, Meharenna, Y., Sligar, S.G. and Poulos, T. L. “Electron Transfer between P450cin and its FMN-Containing Redox Partner” J. Biol. Chem., 2007, 282 (37), 27006-27011
4. Zhao*, J., Das, A.*, Zhang, X, Schatz, G., Sligar, S., Van Duyne, R. “Resonance Surface Plasmon Spectroscopy: Low Molecular Weight Substrate Binding to Cytochrome P450” J. Am. Chem. Soc. 2006, 128 (34), 11004-11005. (* Co-first authors)
(In News): (1) Enhanced LSPR detects binding of small molecules. Anal. Chem., 2006, 78(21), 7356. (2) Kirrill’s Café paper of the month. August 2006
3. Das, A., Trammell, S. A. and Hecht, M. H. “Electrochemical and Binding Studies of an immobilized De Novo Heme Protein” Biophy. Chem. 2006, 123 (2-3), 102-112.
2. Hu, Y., Das, A., Hecht, M. H. and Scoles, G. “Nanografting De Novo Proteins onto gold surface” Langmuir 2005, 21(20), 9103-9109.
1. Hecht, M.H., Das, A., Go, A., Bradley, L. and Wei, Y. (2004) “De Novo Proteins from Designed Combinatorial Libraries.” Protein Science 2004, 13(7), 1711–1723.
Das, A. “Electrochemical and Functional Studies of De Novo Alpha Helical Protein from a Designed Combinatorial Library.” Ph.D Thesis Princeton University November 2005
Das, A. Model Chemistry of Aerobic Acetylene Hydratase Enzymes. M.Sc. Thesis: I.I.T (Kanpur) 1998
Manuscripts In preparation
Das, A* and many others "Recent development in Nanodiscs for Biophysical Studies (2018-2025). Manuscript ready for submission
Eschbach, M.D., Lopez, A.J., Roy, P.R., Anand, D. and Das, A*. "Omega-3 Endocannabinoid Epoxide Enantiomers formed by Human Cytochrome P450s are bioactive." 2025, In preparation