Technical Skills
Protein Design
DDG
Protein / protein affinity modulation
Protein stabilization
Protein stabilization w/introduced disulfides
Linker design for fusion proteins
De novo helical backbone design
Protein Structure Modeling
Protein / protein docking
Loop modeling
Linker modeling
Small molecule druggability analysis
Protein Structure Prediction
Ab initio structure prediction
Homology modeling of globular proteins
Homology modeling of protein / protein complexes
Homology modeling w/ specified templates
Programming/Software
ROSETTA, MOE, Schrodinger (Maestro)
Java
C++
Matlab
Python, PyTorch
Bash/Linux/Vim
X-ray
Crystal screening using Mosquito
Crystal optimization - standing drop, sitting drop, temperature, additives, seeding, soaks
Diffraction - ALS Lawrence Berkeley National Laboratory, NSLS-II Brookhaven National Laboratory
Data processing & refinement - XDS, molecular replacement, Phenix, Coot
Protein purification
Molecular cloning - primer design, vector design, restriction cloning, blunt end cloning, Gibson cloning
Expression - E. coli, mammalian (HEK293)
Purification - sonication, microfluidizer, Ni/Co gravity columns Akta SEC
Analysis - SEC-MALS, CD, SAXS, X-ray
Modifications - non-canonical amino acid incorporation, lysine labeling
Mammalian cell culture, transfection, & characterization
HEK, HeLa, U2-OS, Jurkat cell culture
Non-viral & lentiviral transfection/transduction of DNA/siRNA
Flow cytometry, multicolor FlowJo analysis
Confocal & TIRF microscopy