MitoStats

Analysis of mitochondrial networks in 3DEM in combination with MitoGraph

E Martin-Solana (1), L Casado-Zueras (2), T. E. Torres (2,3,4,5), G. F. Goya (3,4),

M. R. Fernández-Fernández (6,7), JJ Fernández (6,7)

(1) Dept Psychiatry, University of Pittsburgh, Pittsburgh, USA.

(2) Advanced Microscopy Laboratory, University of Zaragoza, Zaragoza, Spain.

(3) Instituto de Nanociencia y Materiales de Aragon (INMA), Zaragoza, Spain.

(4) Dept. Condensed Matter Physics, University of Zaragoza, Zaragoza, Spain.

(5) Dept. Chemistry and Chemical Biology, Rutgers, The State University of New Jersey, USA.

(6) Spanish National Research Council (CSIC/CINN), Oviedo. Spain.

(7) Health Research Institute of Asturias (ISPA), Oviedo. Spain.

Contact: jjfernandez.software @ gmail.com

Description

MitoStats, combined with MitoGraph, is a software tool for the analysis of mitochondrial networks in 3DEM. MitoGraph (Viana et al., https://doi.org/10.1016/bs.mcb.2014.10.003) quantifies the volume of tubular mitochondrial networks by using a graph representation of mitochondria, i.e. the mitochondria are decomposed into edges (branches) and nodes (branch junctions and terminal ends) that represent the skeleton of the mitochondria. MitoStats takes the MitoGraph results and combines them so as to provide structural information (N. edges/branches, volume, length, width) for every individual mitochondrion. The output of MitoStats is an output CSV file that can be fed to any statistical tool to perform statistical analysis. Furthermore, MitoStats also produces an output compatible with the well-known software IMOD to allow 3D visualization and identification of individual mitochondria and their skeletons in the whole 3D volume.

If you use MitoStats in your works, please cite the following article:

Disruption of mitochondrial morphology and dynamics in Huntington's Disease unveiled by in-tissue multiscale 3D electron microscopy.

E Martin-Solana, L Casado-Zueras, TE Torres, GF Goya, MR Fernandez-Fernandez, JJ Fernandez

Acta Neuropathologica Communications 12:88, 2024.

https://doi.org/10.1186/s40478-024-01802-2

and please also cite the MitoGraph reference: https://doi.org/10.1016/bs.mcb.2014.10.003.

Download

Current version: June 2024

Available material:

Documentation in PDF: MitoStats.pdf
Binaries for x86-based platforms under Linux (64 bits).
Binaries for Macs based on Intel and Apple silicon processors under OSX/macOS.
Binaries for x86-based platforms under Windows WSL.

The development of MitoStats has been supported by grants from the Cure Huntington’s Disease Initiative (CHDI), Huntington’s Disease Society of America, Fundacion Ramon Areces and Spanish AEI.