Roy Lab Research

Rocío Ortiz, Pooja Basnett, Ipsita Roy, Iban Quintana, Picosecond laser ablation of polyhydroxyalkanoates (PHAs): comparative study of neat and blended material response, 2019 Polymers accepted, in press.

Sahitya Kumar Avugadda, Maria Elena Materia, Rinat Nigmatullin, David Cabrera, Roberto Marotta, Tamara Fernandez Cabada, Elena Marcello, Simone Nitti, Emilio J. Artés-Ibañez, Pooja Basnett, Claire Wilhelm, Francisco J. Teran, Ipsita Roy, Teresa Pellegrino Esterase-Cleavable 2D Assemblies of Magnetic Iron Oxide Nanocubes: Exploiting Enzymatic Polymer Disassembling To Improve Magnetic Hyperthermia Heat Losses,2019, ACS Chemistry of Materials, 31, 15, 5450-5463

Cristina Pérez-Rivero, J. Pablo López-Gómez, Ipsita Roy, A Sustainable Approach for the Downstream Processing of Bacterial Polyhydroxyalkanoates: State-of-the-art and latest developments, 2019, Bioengineering Journal, Volume 150, 15 October 2019, 107283

Lorena R. Lizarraga-Valderrama, Caroline S. Taylor, Frederik Claeyssens, John W. Haycock, Jonathan C. Knowles and Ipsita Roy Unidirectional Neuronal Cell growth and Differentiation on Aligned Polyhydroxyalkanoate Blend Microfibres with Varying Diameters, Topographical guidance of neurons on bacterial polymers, 2019, Journal of Tissue Engineering and Regenerative Medicine 13(9) 1581-1594- also chosen for journal cover page

Pooja Basnett, Elena Marcello, Barbara Lukasiewicz, Bijal Panchal, Rinat Nigmatullin, Jonathan C. Knowles, Ipsita Roy 2018, Biosynthesis and characterization of a novel, biocompatible medium chain length polyhydroxyalkanoate by Pseudomonas mendocina CH50 using coconut oil as the carbon source. Journal of Materials Science: Materials in Medicine, Nov 30;29(12):179. doi: 10.1007/s10856-018-6183-9.

C. Constantinides, P. Basnett, B. Lukasiewicz, R. Carnicer, E. Swider, Q. A. Majid, M. Srinivas, C. Carr, I. Roy 2018, In Vivo Tracking and 1H/19F MRI of Biodegradable Polyhydroxyalkanoate/Polycaprolactone Blend Scaffolds Seeded with Labeled Cardiac Stem Cells, ACS Applied Materials Interfaces: Aug 1;10(30): 25056-25068.

Barbara Lukasiewicz, Pooja Basnett, Rinat Nigmatullin, Rupy Matharu, Jonathan C. Knowles and Ipsita Roy 2018, Binary Polyhydroxyalkanoate Systems for Soft Tissue Engineering, Acta Biomaterialia 71: 225-234.

G Huerta-Angeles, M Brandejsová, R Nigmatullin, K Kopecká, H Vagnerová, D Šmejkalová, I Roy and V Velebný 2018, Synthesis of graft copolymers based on hyaluronan and poly (3-hydroxy-alkanoates), Carbohydrate polymers 171, 220-228

Gloria Huerta-Ángeles, Kateřina Knotková, Petr Knotek, Ondrej Židek, Martina Brandejsová, Marek Pokorný, Hana Vagnerová, Ipsita Roy, Vladimir Velebný 2018, Aligned nanofibres made of poly(3-hydroxybutyrate) grafted to hyaluronan for potential healthcare applications, Journal of Materials Science: Materials in Medicine 29 (3), 32

R. Rai; J. Roether; J. Knowles; V. Mordan; V. Salih; I. Locke; M. Gordge; Cormick; Mohn; Stark; T. Keshavarz; A.R. Boccaccini, I. Roy, 2016 Highly elastomeric Poly(3-hydroxyoctanoate) based natural polymer composite for enhanced keratinocyte regeneration, International Journal of Polymeric Materials and Polymeric Biomaterials, 66:7, 326-335.

A. Sukan, I. Roy, T. Keshavarz 2017 A Strategy for Dual Biopolymer Production of P(3HB) and γ-PGA, Journal of Chemical Technology and Biotechnology, DOI: 10.1002/jctb.5259

Andrea V. Bagdadi, Maryam Safari, Prachi Dubey, Pooja Basnett, Panagiotis Sofokleous, Eleanor Humphrey, Ian Locke, Mohan Edirisinghe, Cesare Terracciano, Aldo R. Boccaccini, Jonathan C. Knowles, Sian E. Harding, Ipsita Roy, 2016 Poly(3-hydroxyoctanoate), a promising new material for cardiac tissue engineering, Journal of Tissue Engineering and Regenerative Medicine, Sep 30. doi: 10.1002/term.2318. [Epub ahead of print]

Zewen Xu, Sunthar Mahalingam, Pooja  Basnett,  Bahijja  Raimi-Abraham,  Ipsita  Roy, Duncan Craig and Mohan Edirisinghe 2016 “Non-woven Fibrous Poly(ε-caprolactone) Constructs for Anti-microbial and Tissue Engineering Applications”, Macromolecular Bioscience, Volume 301, Issue 8, Pages 922-934.

Akaraonye Everest, Filip Jan, Safarikova Mirka, Salih Vehid, Keshavarz Tajalli, Knowles Jonathan and Roy Ipsita 2016 “Composite scaffolds for cartilage tissue engineering based on natural polymers of bacterial origin; thermoplastic Poly(3-hydroxybutyrate) and micro-fibrillated bacterial cellulose”, Polymer International, Volume 65, Issue 7, July 2016 Pages 780–791

Everest Akaraonye, Jan Filip, Mirka Safarikova, Vehid Salih, Tajalli Keshavarz, Jonathan C Knowles, Ipsita Roy 2016 “P (3HB) Based Magnetic Nanocomposites: Smart Materials for Bone Tissue Engineering” http://dx.doi.org/10.1155/2016/3897592

Francis L., Meng D., Locke I., Knowles JC, Mordan N., Salih V., Boccaccini A.R. and Roy I., 2016, “P(3HB) composite films containing bioactive glass nanoparticles for wound healing applications”; Polymer International, Volume 65 (6) pp. 661-674. ISSN 0959-8103

Kuan Y Ching, Orestis G Andriotis, Siwei L, Pooja Basnett, Bo Su, Ipsita Roy, Rahul S Tare, Bram G Sengers and Martin Stolz 2016, “Nanofibrous Poly(3-hydroxybutyrate)/poly(3-hydroxyoctanoate) scaffolds provide a functional microenvironment for cartilage repair”; Journal of Biomaterial Applications, Jul;31(1):77-91.

Daniele Di Mascolo, Pooja Basnett, Anna Lisa Palange, Marco Francardi, Ipsita Roy and Paolo Decuzzi 2016 ‘Tuning Core Hydrophobicity of Spherical Polymeric Nanoconstructs for Docetaxel Delivery’ Polymer International- Volume 65, Issue 7 Pages 741–746.

Lorena R. Lizarraga-Valderrama, Rinat Nigmatullin, Caroline Taylor, John W. Haycock, Frederik Claeyssens, Jonathan Knowles, Ipsita Roy ‘Nerve Tissue Engineering using blends of Polyhydroxyalkanoates for peripheral nerve regeneration.’2015 Engineering in Life Sciences - Special issue: SMART POLYMERS & BIOTECHNOLOGY (invited paper)- 15(6) 612-621

Rinat Nigmatullin, Peter Thomas, Barbara Lukasiewicz, Hima Puttussery, and Ipsita Roy 2015 “Polyhydroxyalkanoates and their applications in Drug Delivery” Journal of Chemical Technology and Biotechnology, Focus Issue, edited by Dr. Ipsita Roy and Dr. Stephen Mahler 90(7) 1209-1221

Guneet Kaur, Ipsita Roy ‘Strategies for large scale production of Polyhydroxyalkanoates’ 2015, Chemical and Biochemical Engineering Quaterly , Special issue “Poly(hydroxyalkanoate) Biopolyesters: Production, Processing and Performance Aspects” 29(2) 157-172

A. Sukan, I. Roy, T. Keshavarz, 2015, Dual production of biopolymers from bacteria, Carbohydrate Polymers, Volume 126, Pages 47-51

A. Sukan, I. Roy, T. Keshavarz, 2014, Agro-Industrial Waste Materials as Substrates for the Production of Poly(3-Hydroxybutyric Acid) Journal of Biomaterials and Nanobiotechnology, Vol.05 No.04, Article ID:50952,11 pages, 10.4236/jbnb.2014.54027

P. F. Reffatti, I. Roy, M. Odell, T. Keshavarz, 2014, Proteomics analysis of Bacillus licheniformis in response to oligosaccharides elicitors, Enzyme and Microbial Technology, Volume 61, Pages 61-66

Basnett P., Kyon Y.C., Stolz M., Knowles J. C., Boccaccini A.R., Smith C., Locke C.I., Roy I. 2013 “Aspirin loaded PHA films: new materials for biodegradable drug eluting stents.” Bioinspired, Biomimetic and Nanobiomaterials 2(BBN3):141-153.

V. Mourino, J. P. Cattalini, Judith Roether, Prachi Dubey, I. Roy & Aldo Boccaccini, “Composite polymer-bioceramic scaffolds with drug delivery capability for bone tissue engineering” Expert Opinion on Drug Delivery, October 2013, Volume 10 (10): Pages 1353-1365

D. Meng, L. Francis, I. D. Thompson, C. Mierke, H. Huebner, A. Amtmann,

I. Roy, A. R. Boccaccini"Tetracycline-encapsulated P(3HB) microsphere-coated 45S5 Bioglass®-based scaffolds for bone tissue engineering", Journal of Materials Science: Materials in Medicine Volume 24, Issue 12, Pages 2809-2817

Basnett P., Kyon Y.C., Stolz M., Knowles J. C., Boccaccini A.R., Smith C., Locke C.I., Keshavarz T., Roy I. 2013 “Novel Poly (3-hydroxyoctanoate)/ Poly (3-hydroxybutyrate) blends for Medical Application” Reactive and Functional Polymers, 73:1340–1348

P. F. Reffatti, I. Roy, M. Odell, T. Keshavarz, 2013, Evidence for the Involvement of Intracellular Ca2+ Ions in the Elicitation Mechanism of Bacillus licheniformis, Journal of Molecular Microbiology and Biotechnology, Volume 23, Issue-6, Pages 391-395

Rana Amache, Artun Sukan, Maryam Safari, Ipsita Roy, Tajalli Keshavarz, 2013 Advances in PHAs production, Chemical Engineering Transactions, Volume 32, Pages 931-936.

Basnett P., Knowles J. C., Pishbin F., Smith C.,  Keshavarz T., Boccaccini A.R.,  Roy I. 2012 “Novel Poly(3-hydroxyoctanoate)/Bacterial Cellulose Composites as potential  materials for biodegradable stent” Advanced Engineering Materials 14(6) B330–B343.

Lydia Francis, Decheng Meng, Jonathan Knowles, Tajalli Keshavarz , Aldo R. Boccaccini  and Ipsita Roy,  2012, “Controlled Delivery of Gentamicin Using Poly(3-hydroxybutyrate) Microspheres ”International Journal of Molecular Science. 12(7), 4294-4314

Akaraonye E., Moreno C., Knowles J. C., Keshavarz T. and Roy I. 2012, “Poly(3-hydroxybutyrate) production by Bacillus cereus SPV using sugarcane molasses as the main carbon source” Biotechnology Journal 7(2) 293-303.

R. Rai, D. M. Yunos , A. R. Boccaccini, J. C. Knowles,  I. A. Barker,  S. M. Howdle, G. D. Tredwell , T. Keshavarz and I. Roy “Poly-3-hydroxyoctanoate P(3HO), a unique medium chain length polyhydroxyalkanoate homopolymer from Pseudomonas mendocina” 2011 Biomacromolecules, 12 (6), pp 2126–2136.

R. Rai, A. R. Boccaccini , J. C. Knowles, N. Mordon, V. Salih, I. C. Locke, M.Torbati, T. Keshavarz and I. Roy 2011The homopolymer, P(3HO) as a matrix material for soft tissue engineering, Journal of Applied Polymer Science, 122, (6), 3606-3617.

D. Meng, L. Francis, I. Roy, A. R. Boccaccini 2011 “Using Electrophoretic deposition to identify protein charge in biological medium” Journal of Applied Electrochemistry 41:919-923

N. M. Thomson, K. Channon, N. A. Mokhtar, L. Staniewicz, R. Rai, I. Roy, S. Sato, T. Tsuge, A. M. Donald, D. Summers, E. Sivaniah 2011 “Imaging internal features of whole, unfixed bacteria” Scanning 33(2): 59-68.

Tania M. Murphy, Alex Y Nilsson, Ipsita Roy, Anthony Harrop, Keith Dixon, Tajalli Keshavarz 2011, Enhanced intracellular Ca concentrations in Escherichia coli and Bacillus subtilis after addition of oligosaccharide elicitors. Biotechnology Letters Vol. 33 Issue 5, p985-991.

R. Rai, T. Keshavarz, J. A. Roether, A. R. Boccaccini, I. Roy 2010 “Medium chain length polyhydroxyalkanoates, promising new biomedical materials for the future.” Material Science Engineering (Reviews) 72(3): 29-47.

E Akaraonye, T Keshavarz and I Roy 2010 “Production of polyhydroxyalkanoates: the future green materials of choice” Journal of Chemical Technology and Biotechnology 85 (6): 732-743.

L Francis, D Meng, IC Locke, N Mordan, V Salih, JC Knowles, AR. Boccaccini, I Roy 2010 “The influence of tetracycline loading on the surface morphology and biocompatibility of films made from P(3HB) microspheres.”  Advanced Engineering Materials Volume 12 (7): B260-B268.

F. Sorrentino, I. Roy, and T. Keshavarz 2010 “Impact of linoleic acid supplementation on lovastin production in Aspergillus terreus cultures.” Applied Microbiology and Biotechnology, 88:65-73

L. Francis, D. Meng, J.C. Knowles, I. Roy and A. R. Boccaccini 2010 “Multi-functional P(3HB) microsphere/45S5 Bioglass((R))-based composite scaffolds for bone tissue engineering. Acta Biomaterialia”, 6 (7): pp. 2773-2786.

T. Keshavarz and I. Roy 2010 “Polyhydroxyalkanoates: bioplastics with a green agenda”. Current Opinion in Microbiology 13 (3): pp. 321-326.

N. Thomson, I. Roy I., D. Summers, E. Sivaniah 2009 "In vitro production of polyhydroxyalkanoates: achievements and applications", Journal of Chemical Technology & Biotechnology, 85 (6): pp. 760-767.

S. K. Misra, S. P. Valappil, J. Knowles, V. Salih, R. Silva, F. Ohasi, I. Roy, A.R. Boccaccini 2009 “Characterisation of carbon nanotube (MWCNT) containing P(3HB)/bioactive glass composites for tissue engineering applications”, Acta Biomaterialia 6 (3): pp. 735-742.

O. Bretcanu, S, K. Misra, D.M. Yunos, A.R. Boccaccini, I. Roy, T. Kowalczyk, S. Blonski, Tomasz A. Kowalewski 2009 “Electrospun Nanofibrous Biodegradable Polyester Coatings on Bioglass®-Based Glass Ceramics for Tissue Engineering” Materials Chemistry and Physics 118 (2-3): pp. 420-426

S. K. Misra, T. Ansari, D. Mohn, S.P. Valappil, T.J. Brunner, W.J. Stark, Ipsita Roy, J.C. Knowles, P.D. Sibbons, E.V. Jones, A.R. Boccaccini, V. Salih 2009 “Effect of nanoparticle bioactive glass on bioactivity and cytocompatibility of P(3HB) composites.” Journal of Royal Society Interface 7 (44): pp. 453-465

Philip S.E., Sengupta S., Keshavarz T., Roy, I. 2009. Effect of impeller speed and pH on the production of poly(3-hydroxybutyrate). Biomacromolecules 10(4): 691 – 699

Nair R., Roy, I., Bucke C., Keshavarz T. 2009 Quantitative PCR study on the mode of action of oligosaccharide elicitors on penicillin G production by Penicillium chrysogenum Journal of Applied Microbiology 107 (4); pp. 1131-1139

Misra S.K., Philip S., Chrzanowski W., Roy I., Knowles J., Salih V., Boccaccini A.R. 2009 Incorporation of Vitamin E in poly(3-hydroxybutyrate)/Bioglass® composite films: Effect on surface properties and cell attachment- Journal of the Royal Society- Interface 6(33): 401-409.

Bretcanu O., Misra S., Roy I., Renghini C., Fiori F., Beccaccini A.R. 2009 In vitro biocompatibility of 45S5 Bioglass®-derived glass ceramic scaffolds coated with Poly(3-hydroxubutyrate). Journal of Tissue Engineering and Regenerative Medicine 3(2):139-148.

Valappil, S.P., Rai, R., Bucke, C., Roy, I. 2008. The effect of varied nutrient limiting conditions on polyhydroxyalkanoate biosynthesis in Bacillus cereus SPV and an insight into the biosynthetic genes involved. Journal of Applied Microbiology Jun; 104(6):1624-35.

Misra SK, Mohn D, Brunner TJ, Stark WJ, Philip SE, Roy I., Salih V, Knowles JC, Boccaccini AR  2008. Comparison of nanoscale and microscale bioactive glass on the properties of P(3HB)/Bioglass composites. Biomaterials Apr; 29(12):175

Peiris D, Dunn WB, Brown M, Kell, D. B.), Roy, I., Hedger, J. N. 2008 Metabolite profiles of interacting mycelial fronts differ for pairings of the wood decay basidiomycete fungus, Stereum hirsutum with its competitors Coprinus micaceus and Coprinus disseminatus. Metabolomics 4(1) : 52-62

Chouiter R., Roy I. , Bucke C. 2008 Optimisation of beta-glucuronidase production from a newly isolated Ganoderma applanatumm Journal of Molecular Catalysis B-Enzymatic  50 (2-4): 114-120   

Murphy T., Roy I., Harrop A., Dixon K., Keshavarz T. 2008 Elicitation effects of oligosaccharides on the transcriptional level of bacitracin ABC transporter genes in Bacillus licheniformis. Biotechnol. Lett. 30(9): 1665-1670

R Nair, Tania Murphy, Ipsita Roy, T Keshavarz 2008 Optimisation studies on multiple elicitor addition in microbial systems: P. chrysogenum and B. licheniformis, Chem Eng Trans, Volume 14, Pages 373-380

Bretcanu, O., Chen, Q. Z., Misra, S. K., Roy, I., Verne, E., Vitale Brovarone, C., Boccaccini, A. R. 2007 Biodegradable polymer coated 45S5 Bioglass®-derived glass-ceramic scaffolds for bone tissue engineering, Glass Technology: European Journal of Glass Science and Technology Part A 48: 227-234.

Misra S.K., Valappil S.P., Nazhat S.N., Moshrefi-Torbati M, Roy I., Boccaccini A.R., 2007 Fabrication and characterisation of biodegradable poly(3-hydroxybutyrate) composite containing Bioglass® for tissue engineering applications. Biomacromolecules Jul;8(7):2112-9.

Valappil, S.P., Misra, S.K., Boccaccini, A.R., Keshavarz, T., Bucke, C., Roy, I.  2007 Large scale production and efficient recovery of P (3HB) with improved material properties from a newly isolated Bacillus sp. Journal of Biotechnology 132(3) 251-258.

Valappil, S.P., Boccaccini, A.R., Bucke, C., Roy, I. 2007 Polyhydroxyalkanoates in Gram positive bacteria: insights from the genera Bacillus and Streptomyces. Antonie van Leeuwenhoek 91(1):1-17

Valappil, S.P., Peiris, D., Langley, G.J., Herniman, J., Boccaccini, A.R., Bucke, C., Roy, I. 2007 Polyhydroxyalkanoate (PHA) biosynthesis from structurally unrelated carbon sources by a newly characterized Bacillus spp. Journal of Biotechnology Jan 127(3): 475-487

Murphy T, Roy I., Harrop A, Dixon, K., Keshavarz T. 2007 Effect of oligosaccharide elicitors on bacitracin A production and evidence of transcriptional level control Journal of Biotechnology Jan 131(4): 397-403

Misra  S.K., Watts P.C.P.,Valappil S.P., Silva  S.R.P., Roy I., Boccaccini A.R. 2007 Poly(3-hydroxy-butyrate)/Bioglass® composite films containing carbon nanotubes Nanotechnology 18(7): Art No: 075701 doi:10.1088/0957-4484/18/7/075701:

Philip, S. Keshavarz, T., Roy, I. 2007 Polyhydroxyalkanoates: biodegradable polymers with a range of applications. Journal of Chemical Technology and Biotechnology 82 (3):233-247

Bretcanu O, Chen Q, Misra SK, Roy, I., Verne, E., Brovarone, C. V., Boccaccini, A. R. 2007 Optimisation of Bioglass® based scaffolds for bone tissue engineering  Tissue Engineering  13(7): 1737-1737

Murphy T., Parra R, Radman R, Roy, I., Harrop, A., Dixon, K., Keshavarz, T. 2007 Novel application of oligosaccharides as elicitors for the enhancement of bacitracin A production in cultures of Bacillus licheniformis Enzyme and Microbial Technology  40(6): 1518-1523

Valappil S.P., Misra S.K., Boccaccini A.R., Roy I. 2006; Biomedical applications of Polyhydroxyalkanoates (PHAs), an overview of animal testing and in vivo responses. Expert Review in Medical Devices 3(6): 853-868: an invited review

Misra, S.K, Valappil, S.P., Roy, I., Boccaccini, A.R. 2006 Polyhydroxyalkanoate (PHA)/inorganic phase composites for tissue engineering applications Biomacromolecules Aug;7(8):2249-58..

Nair R., Radman R., Roy, I., Bucke C., Keshavarz T. 2005 Towards unravelling the elicitation mechanism in Penicillium chrysogenum: Chrysogenin elicitation. Chemical Engineeering Transactions 6: 989-994:

Shoolingin-Jordan P.M., Al-Daihan S., Alexeev D., Baxter R.L., Bottomley S.S., Kahari I.D., Roy I., Sarwar M., Sawyer L., Wang S.F. 2003; Aminolaevulinic Acid synthase: Mechanism, mutations and medicine Biochim Biophys Acta.Apr11; 1647(1-2): 361-65:

Verma, S., Bhatia, Y., Valappil, S.P., Roy, I. 2002; A possible role of polyhydroxybutyric acid in antibiotic production in Streptomyces. Archives of Microbiology 179, 66-69:

Celis, R.T.F., Leadlay, P.F., Roy, I., Hansen, A., 1998 Phosphorylation of the Periplasmic Binding Protein in Two Transport Systems for Arginine Incorporation in Escherichia coli K12 is unrelated to the Function in the Transport system. Journal of Bacteriology, 180, 4828-4833:

Roy, I., Leadlay, P.F., 1997, Advances in Gene Technology: Biomolecular Design, Form and Function, A Methylmalonyl-CoA mutase in Escherichia coli? Nature Biotechnology Short Reports 8, 48

Roy, I., 1996, The masked cysteine residues in Methylmalonyl-CoA mutase from Propionibacterium shermanii are essential for catalytic activity. FEBS Letters, 394,126-128

Roy, I., Leadlay, P.F., 1992, Physical Map Location of the New Escherichia coli gene sbm. Journal of Bacteriology, 17, 5763-5764