"We have run the Acrometrix OHC, and 2 versions of a Seracare somatic control (AF7 and STML2), on a Nimblegen custom capture library with 500 genes. Those capture pools were sequenced on the Illumina HiSeq 4000"
Targeted sequencing panels validation/performance.
Kristie Jones, CGR Production Manager
AcroMetrix OHC
tumor/normal engineered cell lines with variable impurity
- between known variants, spiking known genomic DNA (e.g., from NA12878) with synthetic DNA to know the true hom ref calls, and targeted sequencing, this data set could stand as a single validation data set for tumor/normal paired calls
- cell line cancer reference sets reviewed here
- Horizon Diagnostics
- engineered cell lines with ~40 cancer-relevant mutations
- uses homologous recombination-based gene engineering to build human somatic cell lines mixed in defined stoichiometric dilutions to provide quantitative standards harboring defined allelic burdens
- SeraCare sells reference sample here