Final Thoughts
Through the processsing from finding a topic of interest to narrowing down my research question, my research on planaria and utilzing in new substance to test for its regenerative capabilites has furthered my knowledge in stem cell regenerative medicine and science compared to before. Additionally, while I have utilized planaria as a model organism in the past, this project has expanded my knowledge in how to manage a planaria and going into in-depth analysis of its purpose in stem cell research. While I was unfortunately unable to get concrete evidence on whether or not ectoine could be a potential method for regenerative medicine in all types of stem cells, I hope to expand on the limited known range of ectoine's potential in regenerative medicine, and possible utilize ecotine on actual specialized stem cells in the near future for research.
Within the data collection and experimental process, I was able to obtain skills that were neccassary and useful throughout my research project. For instance, throughout my research, I had to learn the skills of knowing when pivot and be flexible with new changes to my project, as there were always unexpected delays that had occured, specifically during my data collection process. These skills had not only been applied during my research process, but also during my final presentation where I had to learn to be confident in my own research and data collected even though there were multiple steps that went wrong during the actual data collection process.
Throughout the entirety of this research process, there were many things there many things that I had learned about not only how to conduct research but also I learned a lot about how I work under different types of pressures during research. For instance, with unexpected delays, I initially did not know how to handle these unexpected changes to my research project, however, as I continued to problem solve my way throuhg these challenges, I was able to obtain and further my skills in knowing when to pivot. I also learned how to become more confident in not only my research but also in my public speaking skills. This entire process has challenged me in ways that I would have never expected to be challenged in, which made the experience difficult but worth it despite the hardships!
I would like to thank the following people for guiding and supporting me throughout the entirety of my research process!
Mrs. Dobos
Mr. McBride
Mrs. Petri
Thank you for providing me all the help that was needed to complete my research process, whether that was providing a space to work on my research or giving me academic advice to help improve my research.
For my future plans, I will be continuing my academic journey at CU Boulder where I will be majoring in Molecular, Cellular, & Developmental Biology under the honors program at the College of Arts & Sciences. I'm excited to utilize these experiences that I have taken on during this research and hope to apply this in future careers in the biomedical field!
Click the links below to learn more about my research!
Research Paper: https://docs.google.com/document/d/1gEqEaGQLW2fFmadfqqLDlMrhhx1LgT2xNCvZJfXi-w8/edit?tab=t.0
Presentation: https://www.canva.com/design/DAHE9TdhWLo/kfsGwFsDXqdyD0ki_I5i0A/edit
Stem cell regenerative science has been a pressing field of topic within the research and biomedical community. With improvements in technology, stem cell research has expanded understanding in maintaining the tissues of the body and the repairing system of cells. These observations are beneficial for generating healthy cells that combat disease or for patients that are at high risk of medical issues that require renewal of cells. However, current studies of finding substantial regenerative medicines are limited, with a few options available to those in need of stem cell therapies and medicine. This research aimed to test the regenerative capabilities of ectoine on planarian regeneration, specifically in blastema growth. It was hypothesized that the administration of ectoine will effectively increase blastema growth and regeneration in planaria. To conduct this research, the experimental group was exposed to a 10 mg/L dosage concentration of ectoine; the control group was not exposed to any dosage of ectoine. Each group contained 15 planarians per trial where a head decapitation assay was performed across three trials. Each planaria will be incisioned along the pharynx of the planaria; initial and final measurements will be taken to assess for regeneration. A two sample t-test was used to statistically analyze the effects of ectoine on planarian regeneration, where statistical significance was not achieved. The statistical test generated a p-value of 0.1454 which exceeded the standard significance threshold of 0.05, depicting no statistically significant effect of ectoine on blastema growth and regeneration on planaria.