Evaluating the Effect of G. glabra on Seizure Recovery Time in bs Drosophila melanogaster
Overview
Epilepsy is a disorder of abnormal electrical activity in the brain that causes seizures. It affects millions of people worldwide, but treatment is virtually inaccessible in low-income countries. There are FDA approved Anti-Epileptic Drugs (AEDs) to treat epilepsy, these drugs are expensive and usually inaccessible to people in low-income countries. Thus, the goal of this research is to aid in the effort to find more affordable treatments that will be more attainable for populations struggling with epilepsy in disadvantaged communities. My research will test the effect of licorice root (G. Glabra), a common alternative to AEDs, on seizure recovery time in Julius seizure Drosophila melanogaster, a mutant bang-sensitive (bs) strain. I hypothesize that licorice root will shorten seizure recovery times in the aforementioned Drosophila as compared to the negative control; but be as effective as avobenzone, the positive control. Drosophila will be separated into vials containing these sample groups for 24 hours before inducing seizures and timing the recovery. This will be repeated for 3 trials to accumulate a minimum of 30 data points for each group. A two sample t-test will be performed to test for statistical significance. Drosophila are approved for use in Rock Canyon’s BSL-1 laboratory and all chemicals are approved for use by Kerry Hinton, the chemical safety manager. Avobenzone will be handled under a fume hood and disposed of according to proper safety procedures while wearing appropriate PPE of gloves and goggles at all times.
Video
video Student Rishika K 12_14_2022 12_07_00 AM.mp4