In a variety of important crops, defense mechanisms are mediated through terpene biosynthesis. Terpenes are a very large and diverse class of organic compounds which can be found in almost all plants and numerous animals. Many of those terpenes serve as flavors, fragrances, and medicine in our everyday lives.
Since the prediction of terpenoid gene functions are hindered by large gene families with a high sequence identity, genetic and biochemical functional verification is often required. This leads to the development of modular tools and strategies for reducing the pool of candidate genes and enzymes targeted for biochemical studies. Biochemical characterization of selected diTPS via multi-gene combinatorial expression assays in E. coli and Nicotiana benthamiana revealed diverse functions in the biosynthesis of gibberellin phytohormones and an array of specialized diterpenoids with probable roles in the defense against biotic and abiotic stressors (Tiedge et al., 2020; Muchlinski, Jia, Tiedge, et al., 2021; Wyatt, Zerbe, and Tiedge 2024).
To counteract the destructive effects of climate change and poor farming practices, we are motivated to support sustainable agriculture. Some crops like alfalfa (Medicago sativa L.) have the potential to even regenerate nutrient-poor soils. With a focus on those restorative crops, our lab is exploring the role of terpenoids and other specialized metabolites such as flavonoids for sustainable farming (Liu et al., 2024). Terpenoids also act on multiple levels to promote fully circular agriculture, for example by contributing to plant stress resistance, increasing the nutritional value of crops, and valorization of agro-industrial waste as high-value by-products.
Soil degeneration due to salinization and desiccation is a global problem that increasingly affects arable farming. The deteriorating structure, fertility and biodiversity of the increasingly saline soils have a major impact on the high-quality cultivation of seed potatoes and other crops typically grown in those areas. Our multi-year project “Fathom the salty clay” focuses on biotic soil properties, where the goal is to improve the soil quality of saline clay soils through an innovative combination of cropping plan modifications, making crops less sensitive to salt. The project tracks various aspects of soil quality in relation to yield (salt tolerance) of potato varieties and other crops. In the process, practical knowledge is gathered on how a degenerated saline clay soil can be converted into a vital soil on which sustainable salt-tolerant arable farming can take place.
Potatoes grown at our experimental field site
Climate change is a serious threat to the cultivation of all food and bioenergy crops. Switchgrass (Panicum virgatum L.) is a major next-generation feed stock for environmentally sustainable biofuel production. Switchgrass is native to Northern America where it can grow on marginal lands without competing for cultivable land with other food crops.
However, increasing drought stress may become a major challenge for the rising switchgrass cultivation. There are two switchgrass ecotypes, namely lowland and upland, which can be distinguished based on their distinct environmental adaptability and morphological characteristics: The smaller upland ecotype can grow in cooler climates, whereas the taller lowland ecotype produces more biomass and has a better resistance to drought stress.
To elucidate the thus far unknown adaptive mechanisms that support drought stress resistance, we are comparing these two closely related switchgrass ecotypes. These ecotypes have adapted to different environments, which allows us to identify stress-tolerance-related genes and pathways by correlating differential gene expression with variable metabolomes under specific stress conditions (Tiedge et al., 2022).
Root exudates have an important impact on the microbial community of a plant and therefore can be beneficial to the plants' fitness. To identify and quantify metabolites in root exudates, plants are best grown in reproducible conditions while still trying to maintain conditions close to natural environments.
To that aim, we developed a pipeline along with a set of best practices to study the root and rhizosphere metabolomes alongside the microbial communities (Genesiska et al., 2024).
Nectar is a sugar-rich solution produced by most angiosperm plants to fulfill extensive functions, for example the attraction of pollinators and protection against herbivores. Nectar is produced by and secreted from nectaries, which are highly specialized glands, and the surrounding tissue. The synthesis and secretion of floral nectar has been the subject of several studies which have proposed different models on the biochemical and molecular mechanisms underlying nectar secretion. Of the sugars found in nectar, the most prevalent are sucrose and the hexoses glucose and fructose (Tiedge and Lohaus, 2017). Given that hexoses are typically not components of the phloem sap, which supplies the nectaries with sugar molecules, the proportion of hexoses in nectar depends on the presence and activity of sucrose-cleaving enzymes.
Tobacco (genus Nicotiana) is highly diverse in terms of flower morphology and pollination mode. Some species secrete sucrose-rich nectars while others produce hexose-rich nectars, but the exact nectar composition depends on the pollinator type, flowering time, corolla length and other environmental factors (Tiedge and Lohaus, 2018).
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