Yasuyuki KATO-YAMADA, Rikkyo Univ.

Overview

Welcome to the web page of Yasuyuki KATO-YAMADA at the Department of Life Science, Rikkyo University! Our study is focusing on the structure-function relationship of proteins, especially of ATP synthase.

Research Themes

Biochemical analysis of the regulatory mechanism of ATP synthase by the epsilon subunit

epsilon Subunit is thought to be a regulator of bacterial ATP synthase. One of our main research themes is to figure out the relationship between structure and function of epsilon subunit as the regulatator of ATP synthse.

In particular, we are interested in the role of the ATP binding, which we found, to the epsilon subunit.

We are also carrying out studies focusing on the physiological significance of the regulation of ATP synthase by the epsilon subunit.

Related publications:

Kato, S. et al. (2007) J. Biol. Chem. 282, 37618

Kadoya, F. et al. (2011) Biochem. J. 437, 135

Single molecular analysis of the regulatory mechanism of ATP synthase by the epsilon subunit

There is a regulatory system common to all ATP synthases, which is called "ADP-inhibition." The ADP-inhibition is caused by the tightly bound ADP after ATP hydrolysis reaction. The relationship between ADP-inhibition and the inhibition by the epsilon subunit is obscure since both inhibit ATPase activity, make it difficult to distinguish which mode of the inhibition is responsible for the particular moment. We have shown that inhibition by the epsilon subunit counteracts ADP-inhibition by single-molecular and biochemical analyses.

Related publications:

Haruyama,T. et al. (2010) BIOPHYSICS 6, 59

Mizumoto, J. et al. (2013) PLoS ONE 8, e73888

Conditional uncopling of ATPase and H⁺-pump in ATP synthase

We recently found that depending on the nucleotide binding state of epsilon subunit, the coupling between ATPase and H⁺ pumping is altered. Under certain conditions, the epsilon subunit works as the molecular "clutch." We have been trying to figure out the molecular mechanism of the uncoupling and its physiological significance.

Related publication:

Kadoya, F. et al. (2011) Biochem. J. 437, 135

And more!

Publications

You may find most of my publications here or from PubMed.