Microbiology and Molecular Biology Facility

Make: NINGBO SJILAB 

Model: SKL-5000D 

Sonicators are high-frequency tools (20 kHz) that use ultrasonic energy to agitate particles in liquids. These devices are employed to facilitate a wide variety of processes, such as mixing, cleaning, degassing, cell disruption, and sample preparation. 

Make: Eppendorf  

Model: Centrifuge 5910R  

A low temperature centrifuge is used to determine sedimentation velocity, shape and mass of macromolecules, separation of phases, isolate viruses, organelles, membranes and biomolecules such as DNA, RNA and lipoproteins. This can also be used for phase separation of nanomaterials. 

Make: Bio-Rad 

Model: C1000 Touch Thermal Cycler

Gradient PCR is a technique that allows the empirical determination of an optimal annealing temperature using the least number of steps. This optimization can often be achieved in one experiment. 12 linear gradients in the same run allow to optimize the ideal annealing temperature. Fast ramp rate of up to 6℃/s to realize efficient PCR runs. Personalized account management system provides organized programs and unwanted changes. 

Make: Bio-Rad 

Model: CFX Connect Real Time System 

Real-time PCR is a variation of the PCR assay to allow monitoring of the PCR progress in actual time. PCR itself is a molecular process used to enzymatically synthesize copies in multiple amounts of a selected DNA region for various purposes. It is the technique of collecting data throughout the PCR process as it occurs, thus combining amplification and detection into a single step, using a variety of different fluorescent chemistries that correlate PCR product concentration to fluorescence intensity 

Make: Bio-Rad

Model:

Gel electrophoresis is a technique used to separate mixtures of DNA, RNA, or proteins according to molecular size. DNA samples are loaded into wells (indentations) at one end of a gel, and an electric current is applied to pull them through the gel. DNA fragments are negatively charged, so they move towards the positive electrode. 

Make: Bio-Rad

Model: Gel Doc XR+ 

Gel documentation systems, also known as 'gel docs' or 'gel imagers,' are used to record and analyze the results of gel electrophoresis and membrane blotting experiments. These instruments are necessary for visualizing stained or labeled nucleic acids and proteins in media such as agarose, acrylamide, or cellulose. Gel documentation works in the principle of fluorescence. Nuclei acids stained with a fluorescent substance such as Ethidium Bromide are excited by ultraviolet irradiation and emit fluorescent light.