Week 2

Topic: Metrology - Weight and Volume Measurements

Preparation (reference materials)

Read pages 67 - 103 in lab manual.
Review the Metrology lecture slides (posted in D2L Week 2 Content).
Review lab duty assignments (link in Week 2 Content) and read associated SOPs (if applicable).
Review textbook chapters 16, 17, and 18 as needed.

Turn in Homework #1 (Traits Self-Reflection) before class on Day 1.
- Refer to Week 1 for assignment information.
- Submit via Google Form.
Turn in Homework #2 (Dental Care SOP) before class on Day 2.
- Refer to week 1 for assignment information.
- Submit your document (Word doc or PDF file type) through the Assignments tab in D2L.

Prepare to check out of lab each day.
- Use the "Daily Lab Check-out" Google Form before leaving lab each day.
- Refer to the "Lab map and student assignments" document for lab duty assignments.
- Refer to the "Lab Duty Codes and & SOPs" document for explanations and instructions of assignments. Ask your instructor for clarification or further instructions.
- Complete your assigned lab duty each day before checking out.
Peer Notebook Review.
- See link in D2L, Week 2 Content.
- Spend approximately 20 minutes to read the notebook entries of 2-3 peers and discuss documentation practices.
Review SOPs for balances before use.
Review and discuss student-written SOPs (home dental care).
Practice volume measurements with graduated cylinders, serological pipettes, and micropipettes.
- Practice only; no documentation needed.
- Watch the demonstration videos before practicing (see Reference Links).
- Refer to the documents posted in D2L Week 2 Content for instructions on practice activities.

LE #5 (Good Weighing Practices).
- Activity is based on LE #5 in the manual, with some modification.
- Refer to protocol "Good Weighing Practices" (posted in D2L Week 2 Content.)
- Review the SOP and scan through the manual for the balance(s) at your assigned equipment station before using them.
  - Locate your assignment station location and online SOPs through the Online Student Dashboard (posted on D2L homepage).
- Watch the demonstration video (see Reference Links) before using balances.
- Document this as entry #2 in your lab notebook in Benchling and share your data with the class via the Class Data Google Sheet.
- Be thorough in documentation, especially in regards to materials, so that the procedure is traceable and repeatable.
LE #6, Test 1, 2, and 3 (Balance Linearity).
- Use only an analytical balance. Note the maximum capacity for the balance.
- For items A, B, C, and D, select four weight standards, each in the range of 10 to 25 grams.
- Document this as entry #3 in your lab notebook in Benchling and share your data with the class via the Class Data Google Sheet.
Begin the Gravimetric Analysis assignment.
- Refer to the document "BIT109_Week2_Gravimetric_Analysis" (posted in D2L Week 2 Content).
- Document this as entry #4 in your lab notebook in Benchling.
- There will be more time for this assignment next week. You do not need to complete everything today.

Demonstrate your use of the P-20, P-200, and P-1000 micropipettes to the instructor / lab facilitator.

Entries #2 and 3 are to be completed this week.
Entry #4 (Gravimetric Analysis) is initiated this week. Leave the entry open until next week, when we will complete data collection and class discussions.

Continue to practice measurements of weight using top-loading and analytical balances.
- Transfer the measured volume of a solute (e.g. NaCl) from a weigh boat to a sealed container (e.g. Eppendorf / microfuge tubes, 15 mL or 50 mL conical tubes).
- Aim for weight measurements and transfers with error below 1%.
Continue to practice measurements of volume using graduated cylinders, serological pipettes, and micropipettes.
- Transfer measured volume from one container to another (use the most appropriate size - a beaker or flask for larger volumes, and a conical tube or Eppendorf / microfuge tube for smaller volumes).
- Aim for volume measurements with error below 5% (in all circumstances), and ideally below 1% (may or may not be achievable across all volumes and devices).

On the first day of class, there will be more time to collect data for your gravimetric analysis assignment (if needed), as well as time for data analysis and additional practice with weight and volume measurements.
On the final day of class, we will hold Exam 1. This exam includes a written component and a hands-on lab practical component.

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