Exosome ◎ Targeting ʏ Lab 🌞
外泌體。靶向 實驗室
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Chi-Ling Chiang, Ph.D.
江季霖 博士
Ph.D., Biomedical Sciences
Assistant professor, Institute of Biopharmaceutical Sciences,
National Yang Ming Chiao Tung University
My lab focuses on integrating nanotechnology with biomedicine, particularly in cancer immunology/therapy and regenerative medicine. Our first research direction is developing novel synthetic nanocarriers and engineered targeted exosomes to address gene delivery issues. These exosomes can carry therapeutic mRNA and penetrate tissue/tumor barriers through specific targeting to meet therapeutic needs (Chiang, et al. Blood, 2019; Chiang, et al. Nature Communication, 2023). The second direction involves using micro/nanoscale biochips to capture and study single cancer stem cells or exosomes to explore their cellular biology under treatment (Chiang, et al. Cell Reports, 2022; Hong Li* & CL Chiang*, co-first authors, et al. 2024), and to achieve precise cell editing and reprogramming. With my interdisciplinary background and training in biochemistry, bioengineering, immunology, and hematology, I aim to make a substantial impact in this cutting-edge medical field.
Currently, our lab is working on two main projects: (i) Liquid biopsy and exosome detection: We target exosomes in patient body fluids using biochips for liquid biopsy to track molecular targets of various malignant cancers and identify new therapeutic targets. (ii) Dual-targeting system and RNA drug delivery: We use a dual-targeting system formed by monoclonal antibodies and specific peptide chains to encapsulate therapeutic RNA drugs into extracellular vesicles and deliver them specifically to diseased areas in vivo. These projects focus on two particularly aggressive cancers: highly fibrotic pancreatic cancer and small cell lung cancer. The five-year survival rates for these cancers are only 6% and 2%, respectively, and there are no effective second-line drugs available clinically. Through our research, we hope to provide new treatment options for these challenging cancers and significantly impact clinical treatment strategies and patient outcomes.
References
References
Selected publications
1. CL Chiang, SS A Chen, SJ Lee, KC Tsao, PL Chu, CL Yao, and H Lee, “LPA Induces Erythropoiesis through Activating LPA Receptor 3,” Stem Cells. 2011 Sep 13. (SCI: 7.87)
2. CL Chiang, S Goswami, FW Frissora, Z Xie, PS Yan, R Bundschuh, LA Walker, Xiaomeng Huang, Rajeswaran Mani, XM Mo, S Baskar, C Rader, MA Phelps, G Marcucci, JC Byrd, LJ Lee, N Muthusamy, “ROR1-targeted delivery of miR-29b induces cell cycle arrest and therapeutic benefit in vivo in CLL mouse model. “Blood. 2019 Aug 1;134(5):432-444. (SCI: 25.66)
3. CL Chiang, MH Cheng, CH Lin. “From nanoparticles to cancer nanomedicine: old problems with new solutions. “Nanomaterials. 2021 11 (7), 1727. (SCI: 5.35)
4. CL Chiang, EY Hu, L Chang, J Labanowska, K Zapolnik, X Mo, J Shi, TJ Doong, A Lozanski, PS Yan, R Bundschuh, LA Walker, D Gallego-Perez, W Lu, M Long, S Kim, NA Heerema, G Lozanski, JA Woyach, JC Byrd, LJ Lee, N Muthusamy. “Leukemia-initiating HSCs in chronic lymphocytic leukemia reveal clonal leukemogenesis and differential drug sensitivity,” Cell Reports. 2022 Jul 19;40(3):111115. (SCI: 10.00)
5. CL Chiang, Y Ma, YC Hou, Sinyu Chen, MH Chien, ZX Zhang, WH Hsu, X Wang, J Zhang, H Li, L Sun, PS Yan, S Fallen, I Lee, XY Chen, YS Chu, C Zhang, TS Cheng, E Reategui, W Lu, R Lee, Y Yuan, HC Liu, Kai Wang, Michael Hsiao, CY Huang, YS Shan, AS Lee and LJ Lee. “Dual targeted extracellular vesicles regulate oncogenic genes in advanced pancreatic cancer.” Nature Communication. 14 (1), 6692. (SCI:16.6).
6. Hong Li, CL Chiang (co-first author*), et al., Chi-Ying F. Huang, Cheng-Hsu Wang, Yan-Shen Shan, Chung-Pin Li, Martin Fleisher and L. James Lee, “Exosomal mRNA and microvesicular protein of Glypican 1 in pancreatic cancer diagnosis,” Advanced Science. (IF=17.5).
7. Yifan Ma, Lili Sun, Jingjing Zhang, CL Chiang, et al., Yuan Yuan and L. James Lee, “Exosomal mRNAs for angiogenic–osteogenic coupled bone repair,” Advanced Science, 2302622 (SCI: 17.5)
8. S Goswami, CL Chiang, K Zapolnik, J Nunes, A Ventura, X Mo, Z Xie, LJ Lee, S Baskar, C Rader, JC Byrd, M Phelps, B Bhatnagar, N Muthusamy. “ROR1 targeted immunoliposomal delivery of OSU-2S shows selective cytotoxicity in t (1; 19)(q23; p13) translocated B-cell acute lymphoblastic leukemia,” Leukemia research 118, 106872 2022. (SCI: 3.71)
9. J Pan, CL Chiang, X Wang, P Bertani, Yifan Ma, J Cheng, V Talesara , LJ Lee , W Lu. “Cell membrane damage and cargo delivery in nano-electroporation,” Nanoscale. 2023 Feb 23;15(8):4080-4089. doi: 10.1039/d2nr05575a. “Cell membrane damage and cargo delivery in nano-electroporation,” Nanoscale. 2023 Feb 23;15(8):4080-4089. doi: 10.1039/d2nr05575a. (SCI: 8.31)
10. S Goswami, R Mani, J Nunes, CL Chiang, K Zapolnik, E Hu, F Frissora, XMo, LA Walker, PYan, R Bundschuh, L Beaver, R Devine, YT Tsai, A Ventura, Z Xie, M Chen, R Lapalombella, A Walker, A Mims, K Larkin, N Grieselhuber, C Bennett, M Phelps, E Hertlein, G Behbehani, S Vasu, JC Byrd, N Muthusamy. “PP2A is a therapeutically targetable driver of cell fate decisions via a c-Myc/p21 axis in human and murine acute myeloid leukemia,” Blood. 2022. 139 (9), 1340-1358. (SCI: 25.66)
11. EY Hu, P Do, S Goswami, J Nunes, CL Chiang, S Elgamal, AM Ventura, C Cheney, K Zapolnik, E Williams, R Mani, F Frissora, X Mo, L Waldmeier, RR Beerli, H Peng, C Rader, M Long, U Grawunder, JC Byrd, N Muthusamy. “The ROR1 antibody-drug conjugate huXBR1-402-G5-PNU effectively targets ROR1+ leukemia.” Blood advances 5 (16), 3152-3162. 2021. (SCI: 6.80)
12. EY Hu, JS Blachly, C Saygin, HG Ozer, SE Workman, A Lozanski, TJ Doong, CL Chiang, S Bhat, KA Rogers, JA Woyach, KR Coombes, D Jones, N Muthusamy, G Lozanski, JC Byrd “LC-FACSeq is a method for detecting rare clones in leukemia.” JCI insight 5 (12) 2020 (SCI: 6.20)
13. S Goswami, CL Chiang, K Zapolnik, Z Xie, JL Lee, S Baskar, C Rader, JC Byrd, MA Phelps, B Bhatnagar, N Muthusamy. “ROR1 Targeted Immunoliposomal Delivery of OSU-2S Show Selective Cytotoxicity in t(1;19) Translocated B-ALL.” Blood, The Journal of the American Society of Hematology 134. 2019.
14. Z Yang, J Shi, J Xie, Y Wang, J Sun, T Liu, Y Zhao, X Zhao, X Wang, Y Ma, V Malkoc, CL Chiang, W Deng, Y Chen, Y Fu, KJ Kwak, Y Fan, C Kang, CC Yin, J Rhee, P Bertani, J Otero, W Lu, K Yun, AS Lee, W Jiang, L Teng, BYS Kim, LJ Lee. “Large-scale generation of functional mRNA-encapsulating exosomes via cellular nanoporation.” Nature Biomedical Engineering 4 (1), 69-83. (SCI: 18.95)
15. LC Hsiung, CL Chiang, CH Wang, YH Huang, CT Kuo, JY Cheng, CH Lin, V Wu, HY Chou, DS Jong, H Lee, and A M. Wo, “Dielectrophoresis-based cellular microarray chip for anticancer drug screening in perfusion microenvironments,” Lab on a Chip. 2011; 21;11(14):2333-42. (SCI: 6.26)
16. CT Kuo, CL Chiang, R YJ Huang, H Lee and A M Wo, “Configurable 2D and 3D spheroid tissue cultures on bioengineered surfaces with acquisition of epithelial–mesenchymal transition characteristics,” NPG Asia Materials. 2012; 4, e27; doi:10.1038 /am.2012.50. (SCI: 9.04)
17. CT Kuo, CL Chiang, CH Chang, HK Liu, GS Huang, R YJ Huang, H Lee, CS Huang, A M. Wo “Modeling of cancer metastasis and drug resistance via biomimetic nano-cilia and microfluidics,” Biomaterials, 2014, 1562–1571. (SCI: 7.60)
18. R Mani, Y Mao, F Frissora, CL Chiang, J Wang, Y Zhao, Y Wu, B Yu , R Yan, X Mo, L Yu, J Flynn, J Jones, L Andritsos, S Baskar, C Rader, M Phelps, CS Chen, R Lee, J C. Byrd, LJ Lee. “Tumor antigen ROR1 targeted drug delivery mediated selective leukemic but not normal B cell cytotoxicity in chronic lymphocytic leukemia,” Leukemia, 2014. (SCI: 10.16)
19. Y You, Y Tian, Z Yang, J Shi, KJ Kwak, Y Tong, A P Estania, J Cao, WH Hsu, Y Liu, CL Chiang, BR Schrank, K Huntoon, DY Lee, Ziwei Li, Y Zhao, H Zhang, TD Gallup, JH Ha, S Dong, Xuefeng Li, Y Wang, WJ Lu, E Bahrani, LJ Lee, L Teng, W Jiang, F Lan, BYS Kim, AS Lee. “Intradermally delivered mRNA-encapsulating extracellular vesicles for collagen-replacement therapy,” Nature Biomedical Engineering. 2023 Jan 12. doi: 10.1038/s41551-022-00989-w. (SCI: 18.95)
20. LTH Nguyen, J Zhang, XY Rima, X Wang, KJ Kwak, T Okimoto, J Amann, MJ Yoon, T Shukuya, CL Chiang, N Walters, Y Ma, D Belcher, H Li, AF Palmer, DP Carbone, LJ Lee, E Reátegui. “An immunogold single extracellular vesicular RNA and protein (AuSERP) biochip to predict responses to immunotherapy in non‐small cell lung cancer patients,” J Extracellular Vesicles. 2022 Sep;11(9):e12258. (SCI: 17.33)
21. XY Rima, J Zhang, LTH Nguyen, A Rajasuriyar, MJ Yoon, CL Chiang, N Walters, KJ Kwak, LJ Lee, Eduardo Reátegui. “Microfluidic harvesting of breast cancer tumor spheroid-derived extracellular vesicles from immobilized microgels for single-vesicle analysis,” Lab on a Chip 2022 (13), 2502-2518 (SCI: 7.51)
22. Yi You, Y Tian, Z Yang, J Shi , KJ Kwak , Y Tong, AP Estania , J Cao, WH Hsu, Y Liu, CL Chiang, B Shrank, K Huntoon, D Lee, Z Li, Y Zhao, H Zhang, T Gallup, J Ha, S Dong , X Li, Y Wang, WJ Lu, E Bahrani, LJ Lee, L Teng , Wen J, Feng Len, BYS. Kim and Andrew S. Lee, “Intradermally delivered mRNAencapsulating extracellular vesicles for collagen-replacement therapy” Nature Biomedical Engineering (SCI: 29.2), https://doi.org/10.1038/s41551-022-00989-w (2023).
Tel: +886 2 28267000 #67143
Email: CLChiang@nycu.edu.tw
Location: 2F Biomedical Building, No.155, Sec.2, Linong Street, Taipei 112304, Taiwan (R.O.C.)
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