Resources

plasmid-id

For identifying plasmid IDs and deconvoluting PCR1 barcodes in plasmid ID sequencing protocol

eng-potential-vector

Evaluation of engineering potential in undomesticated microbes with VECTOR.

cultured-microbe-identification

Identify cultured microbes and calculate their purity

brb-libprep

Automated vs manual comparison of amplicon library preparation.

BEVA1.0 plasmids

Modular assembly system for Golden Gate cloning vectors for genetic manipulation of diverse microbes

BEVA2.0 plasmids

An expansion to BEVA1.0 that adds many new origins of replication and tools for deletion construction

Plasmid-IDs

System for measuring rhizobium effectiveness and competitiveness with GFP bioreporter and molecular barcodes

Sino-Plasmid-IDs (upcoming)

Plasmid-ID system adapted for use in Sinorhizobium

VECTOR plasmids (upcoming)

A high throughput system for diverse microbiome isolate evaluation of engineerability

High-Throughput Cultivation and Dilution-to-Extinction Protocol for Field-Grown Crops

A high-throughput cultivation method for isolating and identifying bacteria from field-grown crop plants

Acoustic Handler Library Prep for Amplicon Sequencing (96)

Automated amplicon library preparation using the Echo 525 and KingFisher Apex with a 96-well destination plate

Acoustic Handler Library Prep for Amplicon Sequencing (384)

Automated amplicon library preparation using the Echo 525 and KingFisher Apex with a 384-well destination plate

VECTOR-L

A high throughput system for diverse microbiome isolate evaluation of engineerability

VECTOR-S

A high throughput system for diverse microbiome isolate engineering and isolation