• Reading assignments and videos (2-3 hours) on this Google Site

  • List of reading assignments and videos (~1.5 hours)

• Literature search activity

  • Literature search activity (~1.0 hours)

• Complete pre-workshop survey

  • Survey sent via email (~0.5 hrs)

• Thursday, July 6th - Arrival and welcome

• Friday, July 7th - NIH Mentoring workshop and introduction to the program. Lab safety and instrument orientation.

• Weekend - settle in or explore Raleigh, NC.

• Monday, July 10th - Sample preparation considerations, DNA quality, and quantification.

• Tuesday, July 11th -  Library preparation with Miroculus Miro and Oxford Nanopore Technologies VolTRAX2. MinION and Flongle flow cell loading practice. 

  • • Miro Workshop.

• Wednesday, July 12th - Flow cell loading and sequencing setup on MinION M1kB , M1kC, and GridION. 

  • • ONT Workshop.  

Reading assignments and resources will be shared on the PORE website.

• Thursday, July 13th - Introduction to basecalling with Guppy and Dorado. Basecalling practice.

• Friday, July 14th - EPI2ME and EPI2ME Labs

• Weekend - explore NC State and Raleigh

• Monday, July 17th - Analyses on the Cloud: BV-BRC. Afternoon: sample preparation and quality control practice.

• Tuesday, July 18th - Team project time: library preparation and sequencing. 

• Wednesday, July 19th - Team project time: sequencing and data analyses. 

• Thursday, July 20th - Presentations and wrap-up. 

Reading assignments and resources will be shared on the PORE website.  Sample preparation protocols and videos will be shared on the PORE website.

• Abstract writing and submission for an undergraduate research conference. 

• Complete post-workshop survey and program evaluation activities. 

• Share a video or resource for others to implement the workflow.