1. To diagnose and screening for patient with pheochromocytoma and related neurogenic tumours.

2. Suggested candidates for screening with:

   • Hypertension 

   • resistant hypertension

   • Young hypertension (<40 y/o)

   • CKD indicated

24 hours urine, as per collection

24 hours urine container with 10ml of 10% HCl

1. Details of patient

   • • Full name

     • Identity number

     • Clinical and drug history

2. Details of clinician

   • Full name

   • MMC number

   • Stamp & Signature

Avoid at least one (1) week before urine collection as following:

Food 

Chillies,  Eggplant, Fruit juices, Nuts, Tomato, Pineapple Caffeine (Coffee, Tea etc.), Avocado, Watermelon, Chocolate/Cocao, Kiwi, Banana

2. Drugs

Acedophenetidin, Cocaine & LA (Cathecolamine reuptake inhibitors), Acetaminophen, Dextroamphetamine,  Metaproterenol,  Acetanilide, Ephedrine, Methaneamine,  Alcohol   Ethanol, Monoamine oxidase inhibitor, Aldomet, Gyceril guaicolate, Opiods, α-methylnormetanephrome, Halothane (Anaesthetic gases), P-chlorophenylalanine, Aminophylline, Imipramine, Phenothiazine, Aspirin, Isoproterenol, Prochloroperazine, Auspirone/ buspirone, Labetalol, Promethazine, B-complex vitamin, Levodopa, Quinidine,  Benzodiazepines   Mandelamine, Quinine, Central acting hypertensive drug , Meprobamate, Sotalol, Codeine, Metaclopramide, Trycyclic antidepressents

3. Avoid stress and vigorous exercise.

Label 24 hours urine container with two identifier (Name and identity no - NRIC/MRN) 

Day 1:

Instruct the patient to void first urine in the morning and discard the urine. Collect all urine in a 24 hours container for next 24 hours. Mix the urine with the preservative well in the 24 hours container every time urine voided. Record date & time collection started.

Advice patient do not pass the urine directly into the container due to acid splash risk issue. Keep sample in a cool & dry place during the collection period.

Day 2:

Include the final urine voided into 24 hours urine container. Record date & time completed.

Request form:

1. Stated lab details below on PER-PAT 301 form

1. Address

2. Phone number

3. Email address

Procedure:

1. Mix 24 hours urine with preservative well

2. Measure total volume and pH of the specimen and note on PER-PAT 301 form. Lab personnel are responsible to adjust the pH of the specimen.

3. Transfer 10ml (minimum 3.5ml) aliquot into a plain tube. Please seal the tube with parafilm to avoid leaking.

4. Store specimen under 2 – 8oC (stability up to one month) OR freeze under -20oC (stability up to three months).

Shipping Condition: 2 – 8oC

Rejection criteria for test:

Total specimen volume < 750ml

Specimen pH > 5

Leaking specimen

No specialist signature

No pH & volume stated

Aliquot volume < 3.5ml

Protocol for Requesting and Collecting 24 Hours Urine Metaneprines, Hospital Putrajaya.