Embedded Files
Figure I. Distribution of Dissosteira species used for analysis.
Figure II. Map visualizing the geographic distribution of D. spurcata, D. carolina, D. pictipennis, and D. longipennis used in this study.
Sample Collection and DNA Extraction
Grasshoppers were collected from various locations in the United States, identified to species level based on morphological characteristics, and a map was created showing geographical distribution using R version 4.4.2
Femur tissue was selected for DNA extraction due to its dense muscle content, which facilitates DNA recovery, and genomic DNA was extracted following Sanger protocol
PCR Amplification and Purification of the COII Gene
The cytochrome oxidase subunit II (COII) gene was amplified using polymerase chain reaction (PCR).
Sequence Analysis
Raw sequence chromatograms were manually inspected and edited using Sequencer software, and sequences in the same genus were grouped as contingencies.
Phylogenetic Analysis
Closely related taxa were used as outgroups - Agymnastus venerabilis, Camnula pellicida, Circotettix maculatus, Spharagemon bolli, and Conozoa texana. A phylogenetic tree was constructed using the R package ‘ape’.
Morphological Analysis
Specimens available were taken to the imaging lab for analysis of morphological differences in wings
Page updated
Report abuse