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Food Intake Is Similar in ABA and EXE Mice Across Sessions.
Food Intake Is Similar in ABA and EXE Mice Across Sessions.
Main effect of session, **P < 0.01
No main effect of group, P > 0.05
No significant interaction, P > 0.05
Šidák multiple comparisons test showed significant differences between groups for session 4, **P < 0.01, and 5, *P < 0.05.
Comparable Weight Loss Patterns Across Sexes with Sex-Based Weight Differences.
Increased Running Activity in ABA Mice, Influenced by Sex and Group Over Time.
Note: Due to imbalances in session duration and sex distribution across cohorts, no statistical analyses were conducted to assess sex differences. However, qualitative observations are provided.
ABA Female Mice Exhibit Increased Running Activity In Response to Food Restriction.
ABA Female Mice Exhibit Increased Running Activity In Response to Food Restriction.
Main effect of session, **P < 0.01
Main effect of group, *P < 0.05
Group by session interaction,****P < 0.0001
Šidák multiple comparisons test showed no significant group differences over the 6 sessions.
ABA Female Mice Exhibit Increased Running Activity Prior to Food Presentation.
ABA Female Mice Exhibit Increased Running Activity Prior to Food Presentation.
Main effect of session, **P < 0.01
No main effect of group, P > 0.05
Group by session interaction, ***P < 0.001
Šidák multiple comparisons test showed no significant group differences over sessions 3-6.
ABA Female Mice Exhibit Reduced Activity During Food Presentation.
ABA Female Mice Exhibit Reduced Activity During Food Presentation.
Main effect of group, *P < 0.05
No main effect of session, P > 0.05
Group by session interaction, **P < 0.01
Šidák multiple comparisons test showed no significant group differences over sessions 3-6.
Post-Prandial Activity Develops Differently Over Time in ABA and EXE Female Mice.
Post-Prandial Activity Develops Differently Over Time in ABA and EXE Female Mice.
Main effect of session, *P < 0.05
No main effect of group, P > 0.05
Group by session interaction, **P < 0.01.
Šidák multiple comparisons test showed no significant group differences over sessions 3-6.
Increased Running Activity Observed in ABA Male Mice Following Food Restriction.
Increased Running Activity Observed in ABA Male Mice Following Food Restriction.
ABA Male Mice Show Increased Running Activity Prior to Food Presentation.
ABA Male Mice Show Increased Running Activity Prior to Food Presentation.
EXE Male Mice Showed Greater Running Activity During Food Presentation.
EXE Male Mice Showed Greater Running Activity During Food Presentation.
Post-Prandial Activity tends to Develop Similarly for Male ABA and EXE Mice.
Post-Prandial Activity tends to Develop Similarly for Male ABA and EXE Mice.
Note: Due to the low sample size in the EXE group (n=1), statistical analyses were not conducted for male mice running activity. However, observations are described.
Conclusions
Conclusions
Mice that were restricted to 2 hours of food access per day exhibited significantly increased wheel running activity, particularly in the hours preceding food presentation.
The combination of food restriction and running wheel access led to greater weight loss in the ABA mice compared to the non-restricted EXE mice.
FAA likely plays a crucial role in ABA development for adolescent mice, as indicated by the increased activity levels during their sleep/light cycle and decreased activity levels upon food presentation.
The male mice took longer to acclimate to the running wheel and remained in the ABA paradigm longer, suggesting sex differences in ABA progression.
These findings show that the ABA paradigm in mice successfully induced some behaviors seen in human AN, supporting its use in studying the underlying neurobiology of AN.
We successfully established the activity-based anorexia paradigm in adolescent male and female C57BL/6J mice.
Future studies will investigate the neurobiological mechanisms underlying compulsive hyperactivity in ABA mice to identify potential psychopharmacological interventions to aid in the treatment of AN.
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