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Our laboratory is interested in understanding the in vivo mechanisms of neuron development and function, intending to provide insights into treating neurological diseases.
We primarily use the nematode Caenorhabditis elegans (C. elegans) as the model organism for its amenability to advanced live-animal imaging & genetic manipulation and its rich repertoire of behaviors.
Sparse labeling of C. elegans neurons using specific promoters. (A) Cytosolic (red) and the synaptic vesicle (green) markers are expressed cell specifically in the NSM neuron. (B) Cytosolic marker is driven by Pcla-1M promoter and expressed in two neurons. (C) Mitochondrial marker is expressed in the AIY neuron. Scale bars in A-C, 5 μm.
My Movie-final.mp4C. elegans with different genetic backgrounds. Top left: Wild-type. Top right: Uncoordinated mutant. Bottom left: Roller mutant. Bottom right: Fluorescently labeled C. elegans.
optogenetics movie.mp4Optogenetic manipulation of C. elegans neurons controls its behavior. Worms in the video have channelrhodopsin expressed in the GABAergic neurons. The blue bar on the top right corner indicates blue light, which activates GABAergic neurons and immobilizes worms.
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