Embedded Files
This semester, in Biotechnology, students have investigated a range of topics in the areas of cell biology, genetics and DNA manipulation. The focus of the course has been to understand the fundamentals of these disciplines of Biology. A key focus was developing the skills to apply their knowledge of the structure and function of the DNA molecule to examine how molecular tools and techniques can be used to manipulate the molecule for a particular purpose, and to use this information to analyse the interrelationship between scientific knowledge and its applications in society.
ONline learning Fruit dna extractions
ONline learning Fruit dna extractions
Ryan - DNA Extraction Poster.pdfBadveli_Meghana DNA EXTRACTION ASSIGNMENT.pptxMandarin DNA Extraction.pptxdna extraction poster.pdfPracticing crime scene analysis of dna samples
Practicing crime scene analysis of dna samples
Students worked practiced the principles of restriction enzymes and gel electrophoresis (two biotechnology tools) in the context of a crime scene investigation.
Analysing the size of dye particles
Analysing the size of dye particles
using gel electrophoresis (DIY and e-GEL)
using gel electrophoresis (DIY and e-GEL)
Students DIY constructed a gel electrophoresis tank to compare the size of dye pigment particles as an analog to different sized fragments of DNA. Smaller particles should move further through the gel.
Moving the agarose gel from the purple setting mould into the experiment tank
Micropipetting dye into the wells at one end of the gel
Running an electric current through the gel to get the dye pigments to move
A finished gel example after ~25 minutes. Which dye particle colour is the smallest?
Measuring how far each band of pigment particles has travelled from the starting wells.
Students also ran pigments in an E-Gel, which runs the current at a higher voltage. Here you can see the movement of the pigment particles over 7 minutes and the separation of combination colours (e.g. purple) into their primary pigments (e.g. blue and red- far left lane in the gel).
Page updated
Report abuse