Kanika Phagna
BS-MS Third Year, Biological Sciences
Model organisms : The What and the Why
Organisms (non-human) which are widely used to study biological processes because their maintenance and breeding are easy in a laboratory are called model organisms. We have already met these in the previous article and already know how indispensable they are for varied forms of research. They are easy to study as they are small and have fewer numbers of genes which can be programmed easily. They have genes similar to humans so their genes can be modified and thus, many diseases can be studied. Model organisms can also be used to create highly detailed genetic maps (visual representation of the location of different genes on a chromosome).
Enters, C. elegans, the worm!
C. elegans is one of the popular model organisms. It is a nematode worm which is quite different from humans in anatomy but, it shares many similarities at the molecular level. It is among the most widely used model organisms around the world. It is quite potent as a model organism and has been studied thoroughly. With a lifespan of only 2 weeks, these worms produce offspring in large numbers through self-fertilization. It is also easy to handle because of its small size and modest nutritional requirements.
Emile Maupas discovered C. elegans as Rhabditis elegans. It is the first multicellular organism which got its genome sequenced, so a lot of standard genomic methods have been developed using this organism. In 1963, Sydney Brenner, a prestigious molecular biologist at The Medical Research Council, Cambridge, England, decided to shift his attention in research from bacteria and phage to an animal. He decided to work with C. elegans in the early 1960s after considering and testing a lot of organisms to serve as model organisms for the eukaryotic processes, particularly for behaviour and neurobiology.
A striking feature of the worm is its transparency, which makes it possible to observe the fate of individual cells using simple microscopy, or more advanced techniques such as SPIM microscopy or Light Sheet Microscopy (LSFM). It is grown in large numbers and can be easily screened for effects of novel drugs on complex processes involved in human disease. Many techniques and ideas are initially tested on this worm due to their versatile nature as a model organism. Ageing processes can be investigated using this as it passes through several distinct phases of life which are observed physiologically and genetically. It is also used in the study of neural development because of the availability of a comprehensive connectivity map and only 302 neurons and ~7000 synapses, or junctions between neurons.
This worm is a powerful tool for the pharmaceutical industry too because it is easy to culture and undergoes rapid reproduction. Being small in size (1 mm), assays of more than a hundred worms are possible in a single well of a plate with 96 wells. Due to their transparency, they are suitable to study biological processes in vivo and cellular complexity using fluorescent markers.
An adult hermaphrodite C. elegans
Source : Wikimedia CommonsIn many cases a direct translation of human pathology into its phenotypes is impossible but, sometimes their assays match certain aspects of human pathology. It helps to explain the underlying molecular mechanisms and to deliver new approaches for therapeutic strategies, rather than recapitulating human disease pathology in every detail.
It has helped in a better understanding of the underlying mechanism for several diseases such as Alzheimer's disease, cancer, diabetes and depression. Nematode Scent Detection Test (NSDT) was developed using this worm to provide an accurate, painless, affordable, fast and convenient cancer detection system. Wild type C. elegans displayed chemotaxis towards the urine of cancer patients which contains cancer cell secretions and cancer tissues but not towards the control urine (normal urine). This showed the response of the olfactory neurons of C. elegans, which was more for the urine of cancer patients than the control urine. Whereas, mutants and olfactory neurons-ablated worms did not show any attraction. This test helped in the diagnosis of various cancers at early stages.
Muscles and Neurons of the adult worm, immunofluorescence image
Source : ShutterstockBy now, hopefully you are convinced that this slimy, crawling creature is indeed packed with potential, and can serve as a remarkably useful tool for researchers for years to come!