RESEARCH AND SCHOLARLY ACTIVITIES 

PUBLICATIONS

1. Surrency S, Tarrah S, Thuanayan MA, Kim Y, Patil R, Grafton A, Curtis M, Lialios P, Kotsakis GA, Alimperti S. 3D Peri-Implant Epi-Mucosa-on-a-Chip Reveals Alterations in Epithelial Barrier Function Mediated by Host-Bacteria-Biomaterial Interactions. ACS Biomater Sci Eng. 2025. Epub 20251112. doi: 10.1021/acsbiomaterials.5c01232. PubMed PMID: 41223296. 

2. Lialios P, Kim Y, Trewhella I, Myakala K, Hughes E, Wang X, Levi M, Alimperti S. 3D Endothelium-on-a-Chip Reveals Cx43/LDHA Axis as a Driver of Endothelial Barrier Dysfunction in Diabetic Kidney Disease. bioRxiv. 2025. Epub 20250523. doi: 10.1101/2025.05.19.654627. PubMed PMID: 40475678; PMCID: PMC12139719.

3. Curtis M, Kim Y, Patil R, Zhang X, Jayatilake M, Bulgin O, Lialios P, Surrency S, Tarrah S, Lazaris AK, Grafton A, Williams Jt, Nouraie SB, Bizaki M, Xeron P, Van Keuren E, Seppala JE, Laiakis EC, Kitlinska JB, Alimperti S. Ascorbic Acid Modulates Collagen Properties in Glucocorticoid-Induced Osteoporotic Bone: Insights into Chemical, Mechanical, and Biological Regulation. Adv Healthc Mater. 2025:e02606. Epub 20250925. doi: 10.1002/adhm.202502606. PubMed PMID: 40995752.

4. Alimperti S, Levi M. PLVAP: the new villain in glomerular endothelial cell injury. Am J Physiol Renal Physiol. 2025;329(3):F323–F4. Epub 20250404. doi: 10.1152/ajprenal.00050.2025. PubMed PMID: 40184239.

5. Lipshutz S, Kim Y, Curtis M, Friedrich L, Alimperti S. Development of multiparametric bioprinting method for generation of 3D printed cell-laden structures. Biotechnol Prog. 2025;41(4):e70016. Epub 20250312. doi: 10.1002/btpr.70016. PubMed PMID: 40071586; PMCID: PMC12344548. 

6. Lee EJ, Lialios P, Curtis M, Williams Jt, Kim Y, Salipante P, Hudson S, Esch MB, Levi M, Kitlinska J, Alimperti S. Glucocorticoids Alter Bone Microvascular Barrier via MAPK/Connexin43 Mechanisms. Adv Healthc Mater. 2025;14(7):e2404302. Epub 20250120. doi: 10.1002/adhm.202404302. PubMed PMID: 39831839; PMCID: PMC11912118. 

7. Lialios P, Alimperti S. Role of E-cadherin in epithelial barrier dysfunction: implications for bacterial infection, inflammation, and disease pathogenesis. Front Cell Infect Microbiol. 2025;15:1506636. Epub 20250211. doi: 10.3389/fcimb.2025.1506636. PubMed PMID: 40007608; PMCID: PMC11850337.

8. Serafini RA, Curtis MDE, Alimperti S. Intellectual property training should be embedded in the biomedical education process. Nat Biotechnol. 2024;42(12):1924–5. doi: 10.1038/s41587-024-02499-3. PubMed PMID: 39663476. 

9. Patil R, Alimperti S. Graphene in 3D Bioprinting. J Funct Biomater. 2024;15(4). Epub 2024/04/26. doi: 10.3390/jfb15040082. PubMed PMID: 38667539; PMCID: PMC11051043.  

10. Yang B, Alimperti S, Gonzalez MV, Dentchev T, Kim M, Suh J, Titchenell PM, Ko KI, Seykora J, Benakanakere M, Graves DT. Reepithelialization of Diabetic Skin and Mucosal Wounds Is Rescued by Treatment With Epigenetic Inhibitors. Diabetes. 2024;73(1):120-34. Epub 2023/10/24. doi: 10.2337/db23-0258. PubMed PMID: 37874683; PMCID: PMC10784658.  

11. Lee E, Chan SL, Lee Y, Polacheck WJ, Kwak S, Wen A, Nguyen DT, Kutys ML, Alimperti S, Kolarzyk AM, Kwak TJ, Eyckmans J, Bielenberg DR, Chen H, Chen CS. A 3D biomimetic model of lymphatics reveals cell-cell junction tightening and lymphedema via a cytokine-induced ROCK2/JAM-A complex. Proc Natl Acad Sci U S A. 2023;120(41):e2308941120. Epub 2023/10/02. doi: 10.1073/pnas.2308941120. PubMed PMID: 37782785; PMCID: PMC10576061. 

12. Lee, E.J., P. Salipante, A. Kotula, S. Lipshutz, D.T. Graves and Alimperti S. “Mechanical Regulation of  Oral epithelial barrier function” , Bioengineering,  https://doi.org/10.3390/bioengineering10050517. * Cover Story at Bioengineering: https://www.mdpi.com/2306-5354/10/5

13. Kim Y, Lee EJ, Kotula AP, Takagi S, Chow L, Alimperti S. Engineering 3D Printed Scaffolds with Tunable Hydroxyapatite. J Funct Biomater. 2022;13(2). Epub 2022/04/26. doi: 10.3390/jfb13020034. PubMed PMID: 35466216; PMCID: PMC9036238. 

14. Kim Y, Alimperti S, Choi P, Noh M. An Inkjet Printed Flexible Electrocorticography (ECoG) Microelectrode Array on a Thin Parylene-C Film. Sensors (Basel). 2022;22(3). Epub 2022/02/16. doi: 10.3390/s22031277. PubMed PMID: 35162023; PMCID: PMC8838719. 

15.  Salipante PF, Hudson SD, Alimperti S. Blood vessel-on-a-chip examines the biomechanics of microvasculature. Soft Matter. 2021;18(1):117-25. Epub 2021/11/25. doi: 10.1039/d1sm01312b. PubMed PMID: 34816867; PMCID: PMC9001019. 

16.  Lee EJ, Jain M, Alimperti S. Bone Microvasculature: Stimulus for Tissue Function and Regeneration. Tissue Eng Part B Rev. 2021;27(4):313-29. Epub 2020/09/18. doi: 10.1089/ten.TEB.2020.0154. PubMed PMID: 32940150; PMCID: PMC8390780. 

17. Kim Y, Lee EJ, Davydov AV, Frukhtbeyen S, Seppala JE, Takagi S, Chow L, Alimperti S. Biofabrication of 3D printed hydroxyapatite composite scaffolds for bone regeneration. Biomed Mater. 2021;16(4). Epub 2020/12/01. doi: 10.1088/1748-605X/abcf03. PubMed PMID: 33254152; PMCID: PMC8164647. 

18. Nguyen DT, Lee E, Alimperti S, Norgard RJ, Wong A, Lee JJ, Eyckmans J, Stanger BZ, Chen CS. A biomimetic pancreatic cancer on-chip reveals endothelial ablation via ALK7 signaling. Sci Adv. 2019;5(8):eaav6789. Epub 2019/09/07. doi: 10.1126/sciadv.aav6789. PubMed PMID: 31489365; PMCID: PMC6713506.) 

19. Anastasiadou E, Stroopinsky D, Alimperti S, Jiao AL, Pyzer AR, Cippitelli C, Pepe G, Severa M, Rosenblatt J, Etna MP, Rieger S, Kempkes B, Coccia EM, Sui SJH, Chen CS, Uccini S, Avigan D, Faggioni A, Trivedi P, Slack FJ. Epstein-Barr virus-encoded EBNA2 alters immune checkpoint PD-L1 expression by downregulating miR-34a in B-cell lymphomas. Leukemia. 2019;33(1):132-47. Epub 2018/06/28. doi: 10.1038/s41375-018-0178-x. PubMed PMID: 29946193; PMCID: PMC6327052.

20. McCurley A, Alimperti S, Campos-Bilderback SB, Sandoval RM, Calvino JE, Reynolds TL, Quigley C, Mugford JW, Polacheck WJ, Gomez IG, Dovey J, Marsh G, Huang A, Qian F, Weinreb PH, Dolinski BM, Moore S, Duffield JS, Chen CS, Molitoris BA, Violette SM, Crackower MA. Inhibition of alphavbeta5 Integrin Attenuates Vascular Permeability and Protects against Renal Ischemia-Reperfusion Injury. J Am Soc Nephrol. 2017;28(6):1741-52. Epub 2017/01/08. doi: 10.1681/ASN.2016020200. PubMed PMID: 28062569; PMCID: PMC5461783. 

21. Dang LTH, Aburatani T, Marsh GA, Johnson BG, Alimperti S, Yoon CJ, Huang A, Szak S, Nakagawa N, Gomez I, Ren S, Read SK, Sparages C, Aplin AC, Nicosia RF, Chen C, Ligresti G, Duffield JS. Hyperactive FOXO1 results in lack of tip stalk identity and deficient microvascular regeneration during kidney injury. Biomaterials. 2017;141:314-29. Epub 2017/07/18. doi: 10.1016/j.biomaterials.2017.07.010. PubMed PMID: 28711779; PMCID: PMC5567800. 

22. Alimperti S, Mirabella T, Bajaj V, Polacheck W, Pirone DM, Duffield J, Eyckmans J, Assoian RK, Chen CS. Three-dimensional biomimetic vascular model reveals a RhoA, Rac1, and N-cadherin balance in mural cell-endothelial cell-regulated barrier function. Proc Natl Acad Sci U S A. 2017;114(33):8758-63. Epub 2017/08/03. doi: 10.1073/pnas.1618333114. PubMed PMID: 28765370; PMCID: PMC5565405. 

23. Row S, Liu Y, Alimperti S, Agarwal SK, Andreadis ST. Cadherin-11 is a novel regulator of extracellular matrix synthesis and tissue mechanics. J Cell Sci. 2016;129(15):2950-61. Epub 2016/06/18. doi: 10.1242/jcs.183772. PubMed PMID: 27311482; PMCID: PMC5004872. 

24. Alimperti S, Andreadis ST. CDH2 and CDH11 act as regulators of stem cell fate decisions. Stem Cell Res. 2015;14(3):270-82. Epub 2015/03/17. doi: 10.1016/j.scr.2015.02.002. PubMed PMID: 25771201; PMCID: PMC4439315. 

25. Alimperti S, You H, George T, Agarwal SK, Andreadis ST. Cadherin-11 regulates both mesenchymal stem cell differentiation into smooth muscle cells and the development of contractile function in vivo. J Cell Sci. 2014;127(Pt 12):2627-38. Epub 2014/04/18. doi: 10.1242/jcs.134833. PubMed PMID: 24741067; PMCID: PMC4058109.

26. Alimperti S, Lei P, Wen Y, Tian J, Campbell AM, Andreadis ST. Serum-free spheroid suspension culture maintains mesenchymal stem cell proliferation and differentiation potential. Biotechnol Prog. 2014;30(4):974-83. Epub 2014/03/13. doi: 10.1002/btpr.1904. PubMed PMID: 24616445; PMCID: PMC5081183.

27. Alimperti S, Lei P, Tian J, Andreadis ST. A novel lentivirus for quantitative assessment of gene knockdown in stem cell differentiation. Gene Ther. 2012;19(12):1123-32. Epub 2012/01/14. doi: 10.1038/gt.2011.208. PubMed PMID: 22241174. (IF: 4.1)

28. Tian J, Alimperti S, Lei P, Andreadis ST. Lentiviral microarrays for real-time monitoring of gene expression dynamics. Lab on a Chip. 2010;10(15):1967-75. Epub 2010/06/04. doi: 10.1039/c003153d. PubMed PMID: 20520864. (IF: 7.5)

PATENTS

1.                   U.S. Patent 2021/0260249: “Three-dimensional printed hydroxyapatite composite scaffolds for bone regeneration, precursor compositions and methods of printing.”

2.                 Provisional Patent Application No. 63/636,442: “Components and Methods for making scaffold tissues via a pectin containing sacrificial template.”

 RESEARCH FUNDING

  2024-2025  Toulmin Grant, Role: PI/PD

                     Title: Perineural invasion-on-a-chip model for investigating cancer-nerve crosstalk.

                      Status: on going

2024-2025  Children’s Cancer Foundation, Role: Co-PI

                     Title: Growth-Associated Protein 43 (GAP43) in Ewing Sarcoma Bone Metastasis.

                     Status: on going

2021-2026      T32 AG071745, Role: Mentor

                     Title: Aging and Alzheimer’s Research Training Program.

                     Status: on going

 2024-2026    R21CA294025, Role: PI/PD

                     Title: 3D bioengineered microfluidic platform for research on Ewing sarcoma bone   

                     metastasis.

                     Status: on going

2021-2026   R01DE031046, Role: PI/PD

                       Title: Regulation of Epithelial Barrier

                       Status: on going

2023             NSF 2320636, Role: Co-PI

                          Title: MRI: Track 1 Acquisition of a 3-Dimensional Nanolithography Instrument

                          Status: ongoing

2022-2027         Georgetown University StartUp, Role: PI

                        Title: StartUp-Tissue Engineering and Regenerative Medicine Laboratory

                        Status: ongoing

ii. Previous

  2024-2025      Toulmin Grant, Role: PI/PD

                     Title: Investigating the role of biomechanical cues in diabetic microvasculature.

                      Status: completed

2020-2022  R21 AR076497, Role: PI/PD

                          Title: Engineering 3D biomimetic osteogenesis imperfect models to dissect mechanisms   

                          of N-cadherin mediated osteoblast-endothelial function.

                          Status: completed

2018-2021  American Dental Association Foundation, Role: PI/PD

                           Title: Engineering oral tissue on-a chip models to study diabetic periodontal diseases.

                           Status: completed