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Mice to genetically manipulate PASK levels
Mice to genetically manipulate PASK levels
PASK-bGEO-LacZ
Whole-body knock-out of mouse PASK by insertion of bGeo (Neomycin + LacZ) Cassette. Useful to study various aspects of the developmental consequences of the loss of PASK in whole-body physiology and metabolism. PASK knock-out mice are born viable with an expected Mendelian ratio.
These mice show a clear defect in skeletal muscle regeneration after injury. The loss of PASK in these mice affects the expression of multiple transcription factors playing a central role at various stages along the myogenesis program.
In addition, PASK-KO mice show improved insulin sensitivity, glucose clearance and reduced body weight when challenged with High-Fat Diet (HFD). We are continuing to study these mice in our lab to determine the physiological impact of the loss of systemic PASK expression.
Whole-body knock-out of mouse PASK by insertion of bGeo (Neomycin + LacZ) Cassette. Useful to study various aspects of the developmental consequences of the loss of PASK in whole-body physiology and metabolism. PASK knock-out mice are born viable with an expected Mendelian ratio.
These mice show a clear defect in skeletal muscle regeneration after injury. The loss of PASK in these mice affects the expression of multiple transcription factors playing a central role at various stages along the myogenesis program.
In addition, PASK-KO mice show improved insulin sensitivity, glucose clearance and reduced body weight when challenged with High-Fat Diet (HFD). We are continuing to study these mice in our lab to determine the physiological impact of the loss of systemic PASK expression.
PASK-Floxed
PASK-Floxed mice are generated by inserting a LoxP-cassette flanking Exon 2 of PASK. Crossing these mice with appropriate mice carrying a Cre-transgene (driver) results in the excision of the LoxP cassette along with Exon 2, resulting in deletion and frameshift resulting in the disruption of PASK expression. Versatile Cre-drivers can be used to precisely delete PASK gene with exquisite temporal and cellular control to discover the functional importance of PASK in cell-type specific manner.
PASK-Floxed mice are generated by inserting a LoxP-cassette flanking Exon 2 of PASK. Crossing these mice with appropriate mice carrying a Cre-transgene (driver) results in the excision of the LoxP cassette along with Exon 2, resulting in deletion and frameshift resulting in the disruption of PASK expression. Versatile Cre-drivers can be used to precisely delete PASK gene with exquisite temporal and cellular control to discover the functional importance of PASK in cell-type specific manner.
PASK-Rosa26-hPASK-V5 [Transgenic]
PASK-Transgenic mice are generated by inserting a [LoxP-STOP-LoxP]-hPASK-V5 cassette at Rosa26 locus of C57BL6 mice. Crossing these mice with appropriate mice carrying a Cre-transgene (driver) results in the excision of the LoxP-STOP-LoxP cassette, which induces the expression of human PASK in mice! Versatile Cre-drivers can be used to precisely express human PASK gene with exquisite temporal and cellular control to discover the functional importance of gain of expression of PASK in cell-type specific manner.
Using muscle stem cells isolated from these mice, we showed that the overexpression PASK accelerates the myogenesis program, resulting in a robust, syncytial myotubes even in the absence of appropriate signaling cues.
PASK-Transgenic mice are generated by inserting a [LoxP-STOP-LoxP]-hPASK-V5 cassette at Rosa26 locus of C57BL6 mice. Crossing these mice with appropriate mice carrying a Cre-transgene (driver) results in the excision of the LoxP-STOP-LoxP cassette, which induces the expression of human PASK in mice! Versatile Cre-drivers can be used to precisely express human PASK gene with exquisite temporal and cellular control to discover the functional importance of gain of expression of PASK in cell-type specific manner.
Using muscle stem cells isolated from these mice, we showed that the overexpression PASK accelerates the myogenesis program, resulting in a robust, syncytial myotubes even in the absence of appropriate signaling cues.
Cre-Drivers
Our lab has acquired several Cre-drivers, and are in the process of generating mice with two additional Cre-drivers to allow for a precise control of various Floxed-alleles we have in our lab.
Our lab has acquired several Cre-drivers, and are in the process of generating mice with two additional Cre-drivers to allow for a precise control of various Floxed-alleles we have in our lab.
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