歷年計畫

單分子及單細胞偵測平台的發展(1/3)(113-2113-M-110-015-)

多方面生物問題偵測平台之開發(3/3)(112-2113-M-110-001-)

多方面生物問題偵測平台之開發(2/3)(111-2113-M-110-003-)

多方面生物問題偵測平台之開發(1/3)(110-2113-M-110-017-)

多方面生物問題偵測平台之發展(2/2)(109-2113-M-110-014-)

多方面生物問題偵測平台之發展(1/2)(108-2113-M-110-015-)

利用單分子偵測技術研究核酸分子(3/3)(107-2113-M-110-001-)

利用單分子偵測技術研究核酸分子(2/3)(106-2113-M-110-001-)

利用單分子偵測技術研究核酸分子(1/3)(105-2119-M-110-001-)

利用奈米光學工具去研究染色質與表關遺傳的調控機制(103-2113-M-010-003-MY2)

在單分子層面利用光學奈米工具去研究核小體訊息調控機制(101-2738-M-010-001)

在單分子層面利用光學奈米工具去研究核小體訊息調控機制(101-2113-M-010-005 -MY2)

在單分子層面利用光學奈米工具去研究特定位置基因重組機制 (99-2113-M-010-003 -MY2)

歷年學術著作

Nuclease-induced stepwise photodropping (NISP) to precisely investigate single-stranded DNA degradation behaviors of exonucleases and endonucleases. 

Nucleic Acids Res.2024, 1-15, gkae822 . Hui-Pin Chiu, Chung Han Shen, Jan-Kai Wu, Eric Y.C. Mao, Han-Yi Yen, Yuan-Pin Chang, Chyuan-Chuan Wu, Hsiu-Fang Fan*

Binding Behavior of Human Hepatoma-Derived Growth Factor on SMYD1. J.Phys. Chem. B, 2024, 128(32), 7722-7735, Jan-Kai Wu, Ying-ying Lee, Xin Hung, Yuan-Ping Chang, Ming-Hong Tai, Hsiu-Fang Fan*

Exploring the Cellular Impact of Size-Segregated Cigarette Aerosols: Insights into Indoor Particulate Matter Toxicity and Potential Therapeutic Interventions. Chemical Research in Toxicology, 2024,15, 37(7), 1171-1186. Yu-Xin Shen, Pe-Shuen Lee, Chia C. Wang, Ming-Chu Teng, Jhih-Hong Huang, Hsiu-Fang Fan*

 Influence of Cigarette Aerosol in Alpha-Synuclein Oligomerization and Cell Viability in SH-SY5Y: Implications for Parkinson's Disease. ACS Chem. Neurosci. 2024, 13, 8, 1143–1164 Yu-Xin Shen, Pe-Shuen Lee, Ming-Chu Teng, Jhih-Hong Huang, Chia C. Wang*, Hsiu-Fang Fan*

The influence of coiled-coil motif of serine recombinase toward the directionality. Biophysical Journal, 2023, 122, 24,4656-4669, Yei-Wein Chen, Bo-Yu Su, Gregory D Van Duyne, Paul Fogg*, Hsiu-Fang Fan*. 

Single-molecule tethered particle motion to study protein-DNA interaction, Journal of the Chinese Chemical Society, 2023, 70, 6, 1314-1325. Hsiu-Fang Fan*. 

Bioengineered Bacteriophage-like Nanoparticles as RNAi Therapeutics to Enhance Radiotherapy against Glioblastomas. ACS Nano 2023, 17, 11, 10407–10422. Hao-Han Pang; Chiung-Yin Huang; Pin-Yuan Chen; Nan-Si Li; Ying-Pei Hsu; Jan-Kai Wu; Hsiu-Fang Fan; Kuo-Chen Wei*; Hung-Wei Yang* 

 An environmentally sensitive molecular rotor as a NIR fluorescent probe for the detection of islet amyloid polypeptide Talanta. 2023, 254, 124130 Pin-Han Lin, Chang-Shun Tsai, Chia-Chien Hsu, I-Ren Lee, Yu-Xin Shen, Hsiu-Fang Fan, Yun-Wen Chen, Ling-Hsien Tu*, Wei-Min Liu*

Change in the oligomeric state of -Synuclein variants in living cells. ACS Chem. Neurosci. 2022, 13, 8, 1143–1164 Hsiu-Fang Fan*, Wen-Ling Chen, Yan-Zhow Chen, Jian-Wei Huang, Yu-Xin Shen. 

The regulation mechanism of the C-terminus of RecA proteins during DNA strand-exchange process. Biophysical Journal, 2021, 120(15), 3166-3179, Hsiu-Fang Fan*, Shu Su 

 Exploring the impact of glyoxal glycation on β-amyloid peptide (Aβ) aggregation in Alzheimer's disease. J. Phys. Chem. B. 2021, 125(21), 5559-5571. (Co-first) Kai-Wei Hu , Hsiu-Fang Fan , Han-Chen Lin , Jian-Wei Huang , Yu-Chieh Chen , Cai-Ling Shen , Yao-Hsiang Shih , Ling-Hsien Tu*.

Influence of the C-terminal Tail of RecA Proteins from Alkaline pH-Resistant Bacterium Deinococcus Ficus. ACS Omega, 2020,5, 31, 19868-19876, Hsiu-Fang Fan*, Shu Su, Ying-An Kuo, Cyuan-Ji Chen, 

Unique Growth Pathway in Solution-Solid-Solid Nanowires: Cubic to Hexagonal Phase Transformation. ACS Omega, 2020,5, 29, 18441-18448, Yi-Hsin Liu*, Ho-Ying Chen, Hsiu-Fang Fan, Yu-Hsien Chen, Fudong Wang 

 A bipartite thermodynamic-kinetic contribution by an activating mutation to RDF-independent excision by a phage serine integrase. Nucleic Acids Res. 2020, 9, 48(12), 6413-6430. Hsiu-Fang Fan*, Bo-Yu S, Paul A Rowley, Chien-Hui Ma and Makkuni Jayaram*

 A flp-SUMO hybrid recombinase reveals multi-layered copy control of a selfish genome by post-translational modification. PLoS Genetics, 2019, June, 15(6):e1008193. Chien-Hui Ma, Bo-Yu Su1+, Anna Maciaszek, Hsiu-Fang Fan, Piotr Guga and Makkuni Jayaram*.

A 5'-to-3' Strand Exchange Polarity is Intrinsic to RecA Nucleoprotein Filaments in the Absence of ATP Hydrolysis, Nucleic Acids Res. 2019;47(10):5126-5140. Yu-Hsuan Lin, Chia Chieh Chu, Hsiu-Fang Fan, Pang-Yen Wang, Michael M. Cox and Hung-Wen Li*. (Co-First)

 The synergy between RSC, Nap1 and adjacent nucleosome in nucleosome remodeling, BBA - Gene Regulatory Mechanisms,2019, 1862(2):129-140, Kuan-Wei Hsu, Sih-Yao Chow, Bo-Yu Su, Yi-Han Lu, Cyuan-Ji Chen, Wen-Ling Chen, Ming-Yuan Cheng and Hsiu-Fang Fan*

 Single-molecule tethered particle motion stepwise analysis of site-specific DNA recombination. Micromachines 2018 9(5). 216. Hsiu-Fang Fan*,Chien-Hui Ma and Makkuni Jayaram

Single molecule analysis of PhiC31 integrase-mediated site-specific recombination by tethered particle motion. Nucleic Acids Res. 2016,44(22), 109804-10823. Hsiu-Fang Fan *, Tao-shih Hsieh, Chien-Hui Ma and Makkuni Jayaram*

Sequence-dependent nanometer-scale conformational dynamics of individual RecBCD-DNA complexes. Nucleic Acids Res. 2016,;44(12):5849-5860. Ashley R. Carter, Maasa H. Seaberg, Hsiu-Fang Fan, Gang Sun, Christopher J. Wilds,6 Hung-Wen Li, and Thomas T. Perkins*, 

 An Overview of Tyrosine Site-specific Recombination: From an Flp Perspective, Microbiol Spectr. 2015 Aug;3(4). Makkuni Jayaram, Chien-Hui Ma, Aashiq H Kachroo, Paul A Rowley, Piotr Guga, Hsui-Fang Fan, Yuri Voziyanov

Single molecule TPM analysis of the catalytic pentad mutants of Cre and Flp site-specific recombinases: contributions of the pentad residues to the pre-chemical steps of recombination.  Nucleic Acids Res. 2015,;43(6):3237-3255. Hsiu-Fang Fan,,*, Yong-Song Cheng, Chien-Hui Ma, and Makkuni Jayaram,* 

Histone Chaperone-Mediated Nucleosome Assembly Process, PLoS One. 2015; 10(1): e0115007,Hsiu-Fang Fan*, Zi-Ning Liu, Sih-Yao Chow, Yi-Han Lu, and Hsin Li

The partitioning and copy number control systems of the selfish yeast plasmid: an optimized molecular design for stable persistence in host cells. Microbiol. Spectr. 2014, 2(5),.Yen-Ting-Liu,1 Saumitra  Sau, Chien-Hui Ma, Aashiq H Kachroo, Paul A Rowley, Keng-Ming Chang, Hsiu-Fang Fan, and Makkuni Jayaram*. 

Organization of DNA Partners and Strand Exchange Mechanisms during Flp Site-Specific Recombination Analyzed by Difference Topology, Single Molecule FRET and Single Molecule TPM. J. Mol. Biol., 2014, 426(4), 793-815 Chien-Hui Ma, Yen-Ting Liu, Christos G. Savva,, Paul A. Rowley, Brian Cannon, Hsiu-Fang Fan, Rick Russell, Andreas Holzenburg and Makkuni Jayaram* 

Real-time single-molecule tethered particle motion analysis reveals mechanistic similarities and contrasts of Flp site-specific recombinase with Cre and λ Int. Nucleic Acids Res. 2013,;41(14):7031-47. Hsiu-Fang Fan,,* Chien-Hui Ma, and Makkuni Jayaram, 

  Using single-molecule approaches to study archaeal DNA-binding protein Alba1. Biochemistry, 2013, 52 (44), pp 7714–7722. Yen-Wen Lu, Tao Huang, Cheng-Ting Tsai, Yu-Yung Chang, Hung-Wen Li, Chun-Hua Hsu, and Hsiu-Fang Fan*

The characterization of the nuclear dynamics of syntenin-2, a PIP2 binding PDZ protein, Cytometry A., 2013, 83(9), 866-75. Annelies Geenrerts, Hsiu-Fang Fan, Pascale Zimmmermann *, Yves Engelborghs *.

Metabolism of HeLa cells revealed through autofluorescence lifetime upon infection with enterohemorrhagic Escherichia coli. J. Biomed. OPT.2012, 17(10), 101503, Tatyana Yu Buryakina, Pin-Tzu Su., Syu Wan Jr., C.Allen Chang, Hsiu-Fang Fan., Fu-Jen Kao*. 

Real-time single-molecule tethered particle motion experiments reveal the kinetics and mechanisms of Cre-mediated site-specific recombination. 

Nucleic Acids Res. 2012,;40(13):6208-22. Hsiu-Fang Fan* 

Developing single-molecule TPM experiments for direct observation of successful; RecA-mediated strand exchange reaction. PLoS One. 2011, 6(7), e21359. Hsiu-Fang Fan, Micheal M. Cox, Hung-Wen Li*

 Photoinduced electron transfer of oxazine 1/TiO2 nanoparticles at single molecule level by using confocal fluorescence microscopy. Langmuir, 2010, 26,9050-9060 Yi-Ju Chen, Hsin-Yu Tzeng, Hsiu-Fang Fan, Ming-Shiang Chen, Jer-Shing Huang and King-Chuen Lin*

 Kinetic and thermodynamic investigation of rhodamine B adsorption at solid/solvent interfaces by use of evanescent-wave cavity ring-down spectroscopy. Anal. Chem., 2010, 82,868-877 Ming-Shiang Chen, Hsiu-Fang Fan, and King-Chuen Lin*

  Studying RecBCD Helicase Translocation along Chi-DNA Using Tethered Particle Motion with a Stretching Force. Biophys.J. 2009, 96, 1-9. Hsiu-Fang Fan, Hung-Wen Li*

Characterization of two types of silanol groups on fused-silica surfaces using evanescent-wave cavity ring-down spectroscopy, Anal. Chem., 2007, 79(10), 3654-61, Hsiu-Fang Fan, Fuping Li, Richard N. Zare, King-Chuen Li*.

Catalytic isomerization of quadricyclane using fourier transform near-infrared absorption spectroscopy: diffusion, conversion, and temperature effect, J. Phys.Chem. B, 2006, 110(11)5563-9, Hsiu-Fang Fan, Chia-Yu Chang, Thoi-Long Chin, Tong-Ing Ho, King-Chuen Li* 

 Molecular adsorption at silica/CH3CN interface probed by using evanescent wave cavity ring-down absorption spectroscopy: determination of thermodynamic properties, Anal. Chem., 2006, 78(11), 3573-99, Hsiu-Fang Fan, Chien-Yi Hung, King-Chuen Li*.