(1) To develop a target and non-target methodology using UHPLC-tQ and UHPLC-QToF (Synapt XS), including sample preparation, instrumental analysis, and data processing for investigation of the fate of neuroactive compounds in the digestion and fecal fermentation media.

(2) To increase the knowledge about the fate of neuroactive compounds once they enter the GI and how this affects the gut microbiome.