Method optimization based on 27 neuroprotective and 15 neuro-disrupting chemicals (analytical standards, STD). The experimental design includes four groups of tomatoes: conventional and organic “datterini” (D and DO), conventional plump (PF), and processed tomatoes (PS). Samples were randomly analyzed in full scan (FS) and data dependent acquisition modality (DDA) in positive (+) and negative modes (-).The obtained recoveries of tested compounds were between 67-157% at higher (1.6 µg g-1 d.w.) and 41-130% at lower (0.1 µg g-1 d.w) concentration. Method and instrumental repeatability (relative standard deviation) was <20% and <15%, respectively. For most of compounds, the R2 of linear calibration curve was >0.9 and matrix effect ±60%.