Loop Assembly is a recursive based to DNA fabrication used for cloning large DNA constructs. The universal Loop Assembly (uLoop) is performed in vitro and relies on multiple iterated "loops" to combine a set of transcription units. This method is preferred for DNA fabrication since it is compact, has a simple and assembly scheme, and has streamlined and standardized protocols, making it user-friendly for our design.

Recombineering is a genetic and molecular biology technique based on homologous recombination systems. This process is typically done in vivo, specifically in modified E. coli. Compared to other forms of genetic editing techniques; this process does not require restriction enzymes or ligases with the only design considerations found in the homology arms.

PCR genotyping utilizes primers that bind specifically to a region of interest to give qualitative information about the genome. Its main function is to test the lengths of the regions to ensure no large insertions or deletions occurred. For our project, we will be using gel electrophoresis, and although it does not detect point mutations, this method is a cheap and efficient way to ensure the integrity of our design.