🧪 環境污染物與微生物生態實驗室守則 

Environmental Contaminants and Microbial Ecology Laboratory Guidelines (Check below for English version)

一、研究倫理與誠信

1. 數據紀實原則：實驗原始記錄須詳實記載於學校發放的實驗紀錄簿（實驗室空白簿本用完請至二樓產學研鏈結中心領取）及PI給予的雲端分享硬碟空間，嚴禁事後補填或修改數據。

2. 結果公開與討論：全體定期參與Lab meeting，針對實驗結果與數據進行誠實報告與團隊討論，無法參與共同 meeting 請向老師請假，及考慮更換實驗室。

3. 學術規範遵守：撰寫論文、研討會海報時需遵守正確引用原則，尊重原創與共同作者貢獻。

二、農藥與毒性實驗規範

1. 個人防護裝備：處理農藥、重金屬或有毒溶劑時須穿著實驗衣、戴Nitrile手套、防護眼鏡或面罩。

2. 藥劑標示清楚：實驗室內所有農藥試劑須清楚標示名稱、濃度、使用者與日期，不得使用來歷不明藥劑。

3. 廢棄物管理：農藥與毒性實驗產生的廢液須集中收集，依照「農藥毒性廢液桶」規定分類，不得混入微生物或一般廢液。

三、微生物與生物材料操作

1. 無菌作業規範：培養微生物需在無菌操作台進行，使用前後以酒精擦拭並使用UV燈消毒。

2. 菌種標示與儲存：所有分離菌株與毒性試驗用微生物須明確標示菌株編號、日期、來源與儲存溫度。

3. 交叉污染防止：進行毒性接觸試驗與微生物培養應分區進行，避免交叉污染影響實驗結果。

4. 畢業前所有需保存之功能性菌株需儲存於-80度冰箱 （在沈老師實驗室）。

四、資料與儀器管理

1.     實驗資料備份：每月上傳一次研究進度報告檔案與相關的原始數據 Excel表、分析圖與照片至雲端，並留存紙本在實驗記錄簿。

2. 公用儀器登記制：如HPLC、分光光度計、pH計等分析儀器須事前登記使用時段，操作後須記錄使用狀況。

3. 離開或畢業交接：研究結束前應完成數據整理、菌株或藥劑整理與清單更新，並辦理資料與帳號交接。

五、日常管理與團隊合作

1. 維護整潔環境：實驗後務必清洗玻璃器材、擦拭桌面、恢復操作台整潔，留下安全與友善的空間。每月底進行一次全室清潔，所有成員需共同參與。

2. 實驗意外通報：如發生藥劑濺灑、菌株外洩、儀器異常、個人傷害等，請立即通知指導老師與Alice。

3. 休假與請假制度：連續三日以上缺勤須提早向老師與實驗室同仁說明，並安排工作交接。

4. 實驗合作原則：尊重他人研究工作，若需使用他人試劑、資料、儀器，務必事前告知並獲得同意。

🎓 畢業資料繳交

1.     預定論文口試時間前至少兩個月，須繳交論文初稿給指導教授審閱。

2.     論文檔案、原始數據與圖表，保留 EndNote 或 Mendeley 可編輯的檔案格式（勿刪除格式）。

3.     離校前另需繳交paper草稿。

Environmental Contaminants and Microbial Ecology Laboratory Guidelines

I. Research Ethics and Integrity

·       Data Recording Principles: Experimental raw data must be accurately recorded in the laboratory notebook issued by the school (if all the notebooks in the lab have been used, please apply a new one at the Industry-Academia Collaboration Center, 2nd floor) and the cloud drive provided by the PI. Post-filling or modifying data after experiments is strictly prohibited.

·       Result Disclosure and Discussion: All members must participate regularly in lab meetings to honestly report and discuss experimental results and data. If unable to attend, please request leave from the professor and consider changing laboratories if necessary.

·       Compliance with Academic Standards: When writing theses or conference posters, proper citation rules must be followed to respect original contributions and co-authors.

II. Regulations for Pesticide and Toxicity Experiments

·       Personal Protective Equipment (PPE): When handling pesticides, heavy metals, or toxic solvents, laboratory coats, nitrile gloves, and protective goggles or face shields must be worn.

·       Clear Labeling of Reagents: All pesticide reagents in the laboratory must be clearly labeled with the name, concentration, user, and date. The use of unidentified reagents is prohibited.

·       Waste Management: Waste liquids generated from pesticide and toxicity experiments must be collected separately according to the 'Pesticide Toxic Liquid Waste Barrel' rules and must not be mixed with microbial or general waste liquids.

III. Microorganism and Biological Material Handling

·       Sterile Operation Protocols: Microbial cultivation must be conducted in a sterile operating cabinet, disinfected with alcohol wipes and UV light before and after use.

·       Strain Labeling and Storage: All isolated strains and microbes used in toxicity tests must be clearly labeled with strain number, date, source, and storage temperature.

·       Prevention of Cross-Contamination: Toxicity contact tests and microbial cultivation must be performed in separate areas to avoid cross-contamination. All functional strains to be preserved must be stored in the -80°C freezer (located in Professor Shen's laboratory) before graduation.

IV. Data and Equipment Management

·       Backup of Experimental Data: Research progress reports, original Excel data files, analysis graphs, and photos must be uploaded to the cloud monthly, with a printed copy kept in the laboratory notebook.

·       Shared Equipment Registration: Equipment such as HPLC, spectrophotometers, and pH meters must be booked in advance, and the usage status must be recorded after operation.

·       Exit or Graduation Handover: Before concluding research, members must complete the organization of data, strains, and chemical inventories, and complete data and account handover procedures.

V. Daily Management and Teamwork

·       Maintain Clean Environment: After experiments, glassware must be washed, work surfaces cleaned, and the operation area restored to cleanliness. A full laboratory cleaning is conducted at the end of each month, with all members required to participate.

·       Incident Reporting: In case of chemical spills, strain leaks, equipment malfunctions, or personal injuries, immediately notify the PI and Alice.

·       Leave Policy: Absences longer than three consecutive days must be reported in advance to the PI and lab members, and work handover must be arranged.

·       Principles of Experimental Collaboration: Respect others' research work. Permission must be obtained before using others’ reagents, data, or instruments.

Graduation Data Submission

·       A thesis draft must be submitted to the PI at least two months before the scheduled oral defense.

·       Thesis files, original data, and figures must be retained in an editable format (e.g., EndNote or Mendeley) without deleting reference formatting.

·       Before leaving, a draft paper must also be submitted to the PI.