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Dr En-Pei Isabel Chiang received her PhD in Biochemical & Molecular Nutrition from Tufts University in 2000 (mentor: Jacob Selhub), and post-doctoral training at UC Berkeley in biochemistry and metabolism (mentor: Barry Shane) in 2003. Chiang initiated her career in 2003 as a tenure-track Assistant Professor, rose through the ranks to Associate Professor in 2007, and Professor in 2011. Currently Chiang is a life-time distinguished professor at National Chung Hsing University, Taiwan. Chiang works in the areas of folate mediated one carbon metabolic kinetics, nutrient-genome interactions, as well as fundamental aspects of nutrient metabolism and regulation. The current focus of her research also involves the mechanisms by which inflammatory mediators and anti-inflammatory therapies alter vitamin B6 synthesis and utilization. Her research program has been well funded through competitive extramural research grants since the establishment of her independent research career in 2003 and has published her research works in major nutrition, biochemistry, and medical journals. Up to now her publications have been cited over 1500 times (on average each cited >30); and has given/chaired >50 presentations at the FASEB, Asian Nutrition Congress, International Union of Nutritional Sciences, International Conference on Food Factors, International Symposium on Biocatalysis and Agricultural Biotechnology, and other major international conferences. She has received honors from American Society of Nutrition (E.L.R. Stokstad Award), American Association of Cancer Research (Scholar in Training Award); Arthritis Foundation (Doctoral Dissertation Award), Academia Sinica of Taiwan (Shang-Fa Yang Young Scholar Award), Ministry of Science & Technology of Taiwan (Future Technology Award; Outstanding Young Scholar Grant), Taiwan Food Science & Technology Society (Outstanding Young Scholar); Taiwan Nutrition Society (Prof Chen Award for Young Scholar; Outstanding Researcher) and many others.
chiangisabel@nchu.edu.tw +886-4-2285-3049
https://sites.google.com/email.nchu.edu.tw/chiang-lab-2021/pi?authuser=0
2021
Growth enhancement facilitated by gaseous CO2 through heterologous expression of reductive tricarboxylic acid cycle genes in Escherichia coli.
Lo SC, Chiang EP, Yang YT, Li SY, Peng JH, Tsai SY, Wu DY, Yu CH, Huang CH, Su TT, Tsuge K, Huang CC.*
Fermentation Accepted. Jun 2021.
Full article 全文
MAT2A localization and its independently prognostic relevance in breast cancer patients.
Chu PY, Wu HJ, Wang SM, Chen PM, Tang FY, Chiang EP*(Corresponding author).
Int J Mol Sci (IF 4.556), accepted (2021, May).
https://www.mdpi.com/1117078 Full article 全文
Folinate Supplementation Ameliorates Methotrexate Induced Mitochondrial Formate Depletion In Vitro and In Vivo
Sou NL, Huang YH, Chen DY, Chen YM, Tang FY, Ko HA, Fan YH, Lin YY, et al Chiang EP*(Corresponding author).
Int J Mol Sci (IF 4.556), 22(3), 1350 (2021, Jan)
PMID: 33572934 full article 全文
Treatment of 13-cis Retinoic Acid and 1,25-dihydroxyvitamin D3 Inhibits TNF-alpha-mediated expression of MMP-9 protein and cell invasion through the suppression of JNK pathway and microRNA 221 in human pancreatic adenocarcinoma cancer cells
Chiang, EP* Cheng YH*, Syu JN*, Chao CY, Lin HY, Lin CC, Yang MD, Tsai SY, and Tang FY.
PLoS One. in press (2021 Mar) full article 全文
PMID: 33730072
2020
Tracing Metabolic Fate of Mitochondrial Glycine Cleavage System Derived Formate In Vitro and In Vivo.
Int J Mol Sci (IF 4.556), 21(22), 8808. (2020, Dec).
Tan YL, Sou NL, Tang FY, Ko HA, Yeh WT, Peng JH, Chiang, EP*(Corresponding author).
PMID: 33233834
Docosahexaenoic acid inhibits the proliferation of Kras/TP53 double mutant pancreatic ductal adenocarcinoma cells through modulation of glutathione level and suppression of nucleotide synthesis.
Chiang, EP*, Hung WC*, Lee DY*, Syu JN, Chao CY, Yang MD, Tsai SY, Tang FY.
PLoS One. 2020;15(11):e0241186. doi: 10.1371/journal.pone.0241186. eCollection 2020. (2020 Nov)
PMID: 33137095
Expression of MTDH and IL-10 Is an Independent Predictor of Worse Prognosis in ER-Negative or PR-Negative Breast Cancer Patients
Chu PY; Wang SM; Chen PM; Tang FY; Chiang EPI*(Corresponding author).
J Clin Med. 2020 ;9(10):3153. doi: 10.3390/jcm9103153. (2020 Sep)
PMID: 33003428 Research Hightlight
Decyl caffeic acid inhibits the proliferation of colorectal cancer cells in an autophagy-dependent manner in vitro and in vivo
Chen C, Kuo YH, Lin CC, Chao CY, Pai MH, Chiang EPI, Tang FY.
PLoS One 2020 May 13;15(5):e0232832. doi: 10.1371/journal.pone.0232832. eCollection 2020. (2020 May)
PMID:32401800
Metabolic Pathways Enhancement Confers Poor Prognosis in P53 Exon Mutant Hepatocellular Carcinoma.
Chen PM, Li JR, Liu CC, Tang FY, Chiang EI*(Corresponding author).
Cancer Inform. 2020 Jan 7;19:1176935119899913. doi: 10.1177/1176935119899913. eCollection 2020.
PMID: 31933519
蔣恩沛 CHIANG, EN PEI ISABEL*;蘇雅麗 SOU, NGA LAI;林宜瑩 LIN, YI YING;范翊暄 FAN, YI HSUAN;張怡璟 CHING, CHANG YI; HUANG, CHIEH CHEN
ROC Patent 中華民國專利 I688767 (2020 Mar) LCMS PLATFORM FOR TRACING SAM SYNTHESIS PATHWAY AND USE THEREOF IN DRUG DEVELOPMENT 追蹤Ｓ-腺苷甲硫胺酸合成路徑之液相層析質譜平台及其於藥物開發之應用
2019
MTHFR C677T polymorphism increases MTX sensitivity via the inhibition of S-adenosylmethionine and de novo purine synthesis.
Wang YC, Wu MT, Tang FY, Chen DY, Ko HA, Shane B, Huang WN, Chiang EP*(Corresponding author).
Clin Sci (Lond). 2019 Jan 25;133(2):253-267. doi: 10.1042/CS20180932. (2019 Jan)(IF=5.23)
PMID: 30606816 【學術亮點】MTHFR C677T基因多態性透過抑制S-腺苷甲硫氨酸和嘌呤合成提高疾病修飾抗風濕病藥物敏感性
New use of lycopene and preparation of lycopene based on the same 茄紅素之新用途及基於此新用途之製劑 (2019 Feb)
ROC Patent 中華民國專利 I650123
En-Pei Chiang*, Shyi-Dong Yeh , Feng-Yao Tang, Yan-Shan Wu, San-San Wong, Yi-Chun Chen
2017
N-3 polyunsaturated fatty acids alleviate high glucose-mediated dysfunction of endothelial progenitor cells and prevent ischemic injuries both in vitro and in vivo.
Chiu SC, Chao CY, Chiang EI, Syu JN, Rodriguez RL, Tang FY.
J Nutr Biochem. 2017 Jan 28;42:172-181. doi: 10.1016/j.jnutbio.2017.01.009. (IF=4.668)
Impact of diet-derived signaling molecules on human cognition : exploring thr food-brain axis .
Rodriguez RL, Albeck JG, Taha AY, Ori-Mckenney KM, Recanzone GH, Stradleigh TW, Hernandez BC, Tang FV, Chiang EI*,Cruz-Orengo L. (Corresponding author)
NPJ Sci Food. 2017 Oct 30;1:2. doi: 10.1038/s41538-017-0002-4. eCollection 2017.
PMID: 31304244
S-allylcysteine Improves Blood Flow Recovery and Prevents Ischemic Injury by Augmenting Neovasculogenesis.
Syu JN, Yang MD, Tsai SY, Chiang EI , Chiu SC, Chao CY, Rodriguez RL, Tang FY.
Cell Transplant. 2017 Oct;26(10):1636-1647. doi: 10.1177/0963689717724792.
PMID: 29251114
Dual inhibition of key proliferation signaling pathways in triple negative breast cancer cells by a novel derivative of Taiwanin A.
Chiang EI*, Kuo YH*, Chao CY*, Rodriguez RL, Chou PY, Tsai SY, Pai MH, Tang FY.
Mol Cancer Ther. 2016 Dec 12. pii: molcanther.0011.2016. (2017 Mar)(IF=5.579)
NEW USE OF LYCOPENE AND PREPARATION OF LYCOPENE BASED ON THE SAME 茄紅素之新用途及基於此新用途之製劑
ROC Patent 中華民國專利 I607750
蔣恩沛 CHIANG, EN PEI;葉錫東 YEH, SHYI DONG;唐烽堯 TANG, FENG YAO;黃珊珊 WONG, SAN-SAN;吳晏珊 WU, YAN SHAN;陳怡均 CHEN, YI CHUN
2015
Regulation of Folate-Mediated One-Carbon Metabolism by Glycine N-Methyltransferase (GNMT) and Methylenetetrahydrofolate Reductase (MTHFR).
Wang YC, Wu MT, Lin YJ, Tang FY, Ko HA, Chiang EP*(Corresponding author)..
J Nutr Sci Vitaminol (Tokyo). 2015;61 Suppl:S148-50. doi: 10.3177/jnsv.61.S148.
2014
Lower serum folate is associated with development and invasiveness of gastric cancer.
Lee TY, Chiang EP*, Shih YT, Lane HY, Lin JT, Wu CY. (Corresponding author).
World J Gastroenterol. 2014 Aug 28;20(32):11313-20. doi: 10.3748/wjg.v20.i32.11313.
Caffeic acid derivatives inhibit the growth of colon cancer: involvement of the PI3-K/Akt and AMPK signaling pathways.
Chiang EP*, Tsai SY, Kuo YH, Pai MH, Chiu HL, Rodriguez RL, Tang FY.
PLoS One. 2014 Jun 24;9(6):e99631. doi: 10.1371/journal.pone.0099631.
Eicosapentaenoic acid induces neovasculogenesis in human endothelial progenitor cells by modulating c-kit protein and PI3-K/Akt/eNOS signaling pathways. (IF 4.668)
Chiu SC, Chiang EP*, Tsai SY, Wang FY, Pai MH, Syu JN, Cheng CC, Rodriguez RL, Tang FY.
J Nutr Biochem. 2014 Sep;25(9):934-45. doi: 10.1016/j.jnutbio.2014.04.007.
A novel role of the tumor suppressor GNMT in cellular defense against DNA damage.
Wang YC, Lin WL, Lin YJ, Tang FY, Chen YM, Chiang EP*(Corresponding author).
Int J Cancer. 2014 Feb 15;134(4):799-810. doi: 10.1002/ijc.28420. (IF=6.2)
2013
Clinical use of cyclooxygenase inhibitors impairs vitamin B-6 metabolism.
Chang HY, Tang FY, Chen DY, Chih HM, Huang ST, Cheng HD, Lan JL, Chiang EP*(Corresponding author).
Am J Clin Nutr. 2013 Dec;98(6):1440-9. doi: 10.3945/ajcn.113.064477. (IF=6.703)
Organosulfur garlic compounds induce neovasculogenesis in human endothelial progenitor cells through a modulation of MicroRNA 221 and the PI3-K/Akt signaling pathways.
Chiang EP*, Chiu SC, Pai MH, Wang YC, Wang FY, Kuo YH, Tang FY.
J Agric Food Chem. 2013 May 22;61(20):4839-49. doi: 10.1021/jf304951p.
2012
Low-dose methotrexate inhibits methionine S-adenosyltransferase in vitro and in vivo.
Wang YC and Chiang EP* (*Corresponding author).
Molecular Medicine Accepted. (2010 SCI IF 5.908 Medicine Research & Experimental top 10%) NSC97-2320-B-005-008-MY3 and NSC100-2628-B-005-002-MY4
Serine Hydroxymethyltransferase anchors the de novo thymidylate synthesis pathway to the nuclear lamina for DNA synthesis.
Anderson D, Woeller C, Chiang EP, Shane B, Stover PJ.
Journal of Biological Biochemistry (2010 SCI 5.328, Biochemistry & Molecular Biology 50/286) 20
Consumption of high-fat diet induces tumor progression and epithelialmesenchymal transition of colorectal cancer in a mouse xenograft model.
Tang FY, Pai MH, Chiang EP.
Journal of Nutritional Biochemistry (IF=4.668) (2012 Jan)
2011
GNMT expression increases hepatic folate contents and folate-dependent methionine synthase-mediated homocysteine remethylation.
Wang YC, Chen YM, Lin YJ, Liu SP, Chiang EP* (*Corresponding author).
Molecular Medicine (SCI 2010 IF 5.908 Medicine Research & Experimental Top 10%). NSC97-2320-B-005-008-MY3
Blood lipid profiles and peripheral blood mononuclear cell cholesterol metabolism gene expressions in patients with and without methotrexate treatment
Chen DR, Chih HM, Lan JL, Chang HY, Chen WW, Chiang EP*(Corresponding author).
BMC Medicine. 2011 Jan 13;9:4. (IF 9.008)
Glycine-N methyltransferase expression in HepG2 cells is involved in methyl group homeostasis by regulating transmethylation kinetics and DNA methylation.
Wang YC, Tang FY, Chen SY, Chen YM, Chiang EP* (*Corresponding author).
Journal of Nutrition 2011 May;141(5):777-82. (SCI 2010 IF 4.295 Nutrition & Dietetic 11%). NSC97-2320-B-005-008-MY3
Long-term prednisolone treatments increase bioactive vitamin B6 synthesis in vivo.
Chang HY, Tzen JT, Lin SJ, Wu, YT, Chiang EP*(Corresponding author).
Journal of Pharmacology & Experimental Therapeutics 2011 Apr;337(1):102-9. (SCI 2010 IF 4.017 Pharmacology & Pharmacy 15%) NSC97-2320-B-005-008-MY3 andDOH 97-TD-D-113-97011
Low-dose methotrexate treatment is associated with increased total cholesterol and HDL-cholesterol in blood: a pilot study
Chih HM, Chang HY, Chen WW, Chen DR, Lan JL, Chiang EP*(Corresponding author).
Nutrition Science Journal 2010.Dec. DOH 97-TD-D-113-97011
S-Allylcysteine inhibits tumor progression and the epithelial-mesenchymal transition in a mouse xenograft model of oral cancer.
Pai MH, Kuo YH, Chiang EP, Tang FY.
British Journal of Nutrition. 2011 Oct 20:1-11.
2010
Isatis indigotica induces hepatocellular cancer cell death via caspase- independent, apoptosis inducing factor translocation apoptotic pathway in vitro and in vivo.
Chuang YC, Tang FY, Liao JW, Chuang CH, Jong TT, Chen SS, Tsai CH, Chiang EP* (Corresponding author).
Integrative Cancer Therapy. (2011 Mar).
PMID:21382959
Consumption of S-allylcysteine inhibits the growth of human non-small cell lung carcinoma in a mouse xenograft model.
Tang FY*, Chiang EP* (corresponding author), and Pai MH.
Journal of Agricultural and Food Chemistry (Agriculture Multidisciplinary 4.5%, Top 2)
PMID:20866043
2009
Effects of insulin and glucose on cellular metabolic fluxes in homocysteine transsulfuration, remethylation, S-adenosylmethionine synthesis, and global deoxyribonucleic acid methylation.
Chiang EP*(Corresponding author), Wang YC, Chen WW, Tang FY.
Journal of Clinical Endocrinology & Metabolism 2009 Mar;94(3):1017-25. (2010 SCI IF 6.495 Endocrinology & Metabolism 11%) supported by NSC97-2320-B-005-008-MY3) (cited by Cell Metabolism).
PMID:19088160
S-allylcysteine modulates the expression of E-cadherin and inhibits the malignant progression of human oral cancer
Tang FY, Chiang EP and Chung JG, Lee HZ, Hsu CY.
Journal of Nutritional Biochemistry 2009 Dec;20 (12) : 1013-20. (IF 4.668)
PMID:19088160
The methylenetetrahydrofolate reductase C677T mutation induces cell specific changes in genomic DNA methylation and uracil misincorporation: A possible molecular basis for the site-specific cancer risk modification.
Sohn KJ, Jang H, Campan M, Weisenberger DJ, Dickhout J, Wang YJ, Cho RC, Yates Z, Lucock M, Chiang EP, Austin RC, Choi SW, Laird PW, Kim YI.
International Journal of Cancer. 2009 May 1;124(9):1999-2005. (IF 6.2).
PMID:19123462
2007
Folate restriction and methylenetetrahydrofolate reductase 677T polymorphism decreases adoMet synthesis via folate dependent remethylation in human transformed lymphoblasts.
Chiang EP*(Corresponding author), Wang YC, Tang FY.
Leukemia (2017 SCI IF 10.023) 2007 Apr;21(4):651-8.
PMID:17301815
Glycine N-methyltransferase-/- mice develop chronic hepatitis and glycogen storage disease in the liver
Liu SP, Li YS, Chen YJ, Chiang EP, Li AF, Lee YH, Tsai TF, Hsiao M, Hwang SF, Chen YM.
Hepatology (2010 SCI IF 12.845 Gastroenterology & Hepatology Top 2, 2/71) 2007 Oct 15;46(5):1413-1425 NSC95-3112-B-010-010 (PMID:17937387)
Resveratrol inhibits heregulin-beta1 mediated matrix metalloproteinase 9 expression and cell invasion in human breast cancer cells.
Tang FY, Chiang EP.
Journal of Nutritional Biochemistry . 2008 19: 287-294. (IF=4.668)
PMID:17651959
Elevated plasma osteopontin associated with gastric cancer development, invasion and survival.
Wu CY, Wu MS, Chiang EP, Wu, CC, Chen YJ, Chen CJ, Chi NH, Chen GH, Lin JT.
Gut 2007 Jun;56(6):782-9. (IF 19.819)
PMID:17148500)
Plasma matrix metalloproteinase-9 level is better than serum matrix metalloproteinase-9 level to predict gastric cancer evolution.
Wu CY, Wu MS, Chiang EP, Chen YJ, Chen CJ, Chi NH, Shih YT, Chen GH, Lin JT.
Clinical Cancer Research 2007 Apr 1;13(7):2054-60 (IF 10.107)
PMID:17404086
Green tea catechin inhibits ephrin-A1-mediated cell migration and angiogenesis of human umbilical vein endothelial cells
Tang FY, Chiang EP, Shih CJ
Journal of Nutritional Biochemistry 18(6) 391-399 (IF 4.668)
PMID:17049832
2006
Regulation of folate-mediated one-carbon metabolism by 10-formyltetrahydrofolate dehydrogenase.
Anguera MC, Field MS, Perry C, Ghandour H, Chiang EP, Selhub J, Shane B, Stover PJ.
Journal of Biological Chemistry (2010 SCI IF 5.328, Biochemistry & Molecular Biology 50/286) 2006 Jul.7;281(27):18335-42. (cited by Science in 2010)
PMID:16627483
2005
Stempak JM, Sohn KJ, Chiang EP, Shane B, Kim YI. Cell and stage of transformation -specific effects of folate deficiency on methionine cycle intermediates and DNA methylation in an in vitro model Carcinogenesis (2010 SCI IF 5.402 Oncology 27/184) 2005 May;26(5):981-90. Supported in part by NSC-93-2320- B-005-006
PMID:15695236
Chiang EP* (Corresponding author), Smith DE, Selhub J, Dallal G, Wang YC, and Roubenoff R. Inflammation causes tissue specific depletion of vitamin B6. Arthritis Research & Therapy (2010 SCI IF 4.357) 2005 Sep 7(6)R1254-1262 Supported in part by NSC 94-2320-B005-009 Highlighted article
PMID:16277678
Pyridoxine supplementation corrects vitamin B6 deficiency but does not improve inflammation in patients with rheumatoid arthritis.
Chiang EP*(Corresponding author), Selhub J, Bagley P, Dallal G, and Roubenoff R.
Arthritis Research & Therapy (2010 SCI IF 4.357) 2005 Oct 7:1404-1411. NSC 94-2320-B005-009 Highlighted article
PMID:16277693
2003
Abnormal vitamin B(6) status is associated with severity of symptoms in patients with rheumatoid arthritis.
Chiang EP*(Corresponding author), Bagley P, Selhub J, Nadeau M, and Roubenoff R.
American Journal of Medicine (SCI 2010 IF 5.115 Medicine, General & Internal Top 9%)
PMID:12681455
Plasma pyridoxal 5'-phosphate concentration is correlated with functional vitamin B-6 indices in patients with rheumatoid arthritis and marginal vitamin B-6 status
Chiang EP*(Corresponding author), Roubenoff R, Selhub J, Bagley PJ.
Journal of Nutrition 133:1056-1059 (SCI 2010 IF 4.295 Nutrition & Dietetics 11%
PMID:12672918
2002
Cytoplasmic serine hydroxymethyltransferase mediates competition between folate–dependent deoxyribonucleotide and S-adenosylmethionine biosyntheses.
Herbig K, Chiang E-P, Lee LR, Hills J, Shane B and Stover PJ.
Journal of Biological Chemistry (2010 SCI 5.328, Biochemistry & Molecular Biology 50/286) 2002 Oct. 277 (41):38381-9.
PMID:12161434 (cited>180)