• Atsuki Takagi, Takayoshi Tagami, and Masayuki Okuyama. 

Mutant β-fructofuranosidase synthesizing blastose [β-D-Fruf-(2→6)-D-Glcp].

Enzyme Microb. Tech. 180, 110500 (1-9) (2024).

doi: 10.1016/j.enzmictec.2024.110500. PMID: 

• Hye-jin Kang, Takayoshi Tagami, and Masayuki Okuyama.

Substrate Specificity of GH29 α-L-Glucosidases from Cecembia lonarensis. 

J. Appl. Glycosci., 71, 91-94 (2024)

doi: 10.5458/jag.jag.JAG-2024_0004. PMID: 

• Takayoshi Tagami

Structural insights into starch-metabolizing enzymes and their applications. 

Biosci. Biotechnol. Biochem., 88，864-871 (2024)

doi: 10.1093/bbb/zbae069. PMID: 38806254

• Wataru Saburi, Takayoshi Tagami, Takuya Usui, Jian Yu, Toyoyuki Ose, Min Yao, and Haruhide Mori.

Molecular mechanism for the substrate specificity of Arthrobacter globiformis M6 α-glucosidase CmmB, belonging to glycoside hydrolase family 13 subfamily 30.

Food Bioscience, 61, 104516 (2024)

doi: 10.1016/j.fbio.2024.104516.

• 奥山正幸，田上貴祥

自然界にはα-L-グルコシド加水分解酵素が存在する α-L-グルコシダーゼの発見と構造・機能解析

化学と生物，62，109-111 (2024)

リンク：https://katosei.jsbba.or.jp/index.php

• Takashi Tanida, Takayoshi Tagami, Hiroko Sato, Hay Mar Kyaw, Takeshi Fujikawa, Masashi Nagano, Kenji Momozawa, Yojiro Yanagawa, and Seiji Katagiri.

Effects of recombinant osteopontin expressed in Escherichia coli on the recovery of the endometrial epidermal growth factor profile and fertility in repeat breeder dairy cows.

Theriogenology, 217, 159-168 (2024)

doi: 10.1016/j.theriogenology.2024.01.011. PMID: 38280277

• Weeranuch Lang, Debashish Mondol, Aphichat Trakooncharoenvit, Takayoshi Tagami, Masayuki Okuyama, Tohru Hira, Nobuo Sakairi, Atsuo Kimura.

Formulation and evaluation of a novel megalomeric microemulsion from tamarind seed xyloglucan-megalosaccharides for improved high-dose quercetin delivery.

Food Hydrocoll., 137, 108430 (2023)

doi: 10.1016/j.foodhyd.2022.108430. PMID: 

• Rikako Shishiuchi, Hyejin Kang, Takayoshi Tagami, Yoshitaka Ueda, Weeranuch Lang, Atsuo Kimura, and Masayuki Okuyama.

Discovery of α‐L‐Glucosidase Raises the Possibility of α‐L‐Glucosides in Nature.

ACS Omega, 7, 47411-47423 (2022)

doi: 10.1021/acsomega.2c06991. PMID: 36570207

• Kumagai Y, Kishimura H, Lang W, Tagami T, Okuyama M, Kimura A. 

Characterization of an unknown region linked to the glycoside hydrolase family 17 β-1,3-glucanase of Vibrio vulnificus reveals a novel glucan-binding domain.

Mar. Drugs, 20, 250 (2022)

doi: 10.3390/md20040250. PMID: 35447923

• Kyaw HM, Sato H, Tagami T, Yanagawa Y, Nagano M, Katagiri S.

Effects of milk osteopontin on the endometrial epidermal growth factor profile and restoration of fertility in repeat breeder dairy cows. Theriogenology, 184, 26-33 (2022)

doi: 10.1016/j.theriogenology.2022.02.008. PMID: 35255245.

• Weeranuch Lang, Yuya Kumagai, Shinji Habu, Juri Sadahiro, Takayoshi Tagami, Masayuki Okuyama, Shinichi Kitamura, Nobuo Sakairi, Atsuo Kimura.

Physicochemical functionality of chimeric isomaltomegalosaccharides with α-(1 → 4)-glucosidic segments of various lengths.

Carbohyd. Polym., 291, 119562 (2022)

doi: 10.1016/j.carbpol.2022.119562; PMID: 35698333.

• Tagami T, Chen M, Furunaga Y, Kikuchi A, Sadahiro J, Lang W, Okuyama M, Tanaka Y, Iwasaki T, Yao M, Kimura A.

Structural insights reveal the second base catalyst of isomaltose glucohydrolase.

FEBS J., 289, 1118-1134 (2022)

doi: 10.1111/febs.16237. PMID: 34665923.

• Lang W, Kumagai Y, Sadahiro J, Saburi W, Sarnthima R, Tagami T, Okuyama M, Mori H, Sakairi N, Kim D, Kimura A.

A practical approach to producing isomaltomegalosaccharide using dextran dextrinase from Gluconobacter oxydans ATCC 11894. 

Appl. Microbiol. Biotechnol., 106, 689-698 (2022)

doi: 10.1007/s00253-021-11753-6. PMID: 35024917.

• Ma M, Okuyama M, Tagami T, Kikuchi A, Klahan P, Kimura.

α-1,3/α-1,4-Glucosidase from Aspergillus niger exhibits unique transglucosylation to generate high levels of nigerose and kojibiose.

J. Agric. Food. Chem., 67, 3380-3388 (2019)

doi: 10.1021/acs.jafc.8b07087. PMID: 30807133.

• 田上 貴祥

放線菌Kribbella flavidaによる環状四糖の生成と代謝

バイオサイエンスとインダストリー, 75, 140-141 (2017)

• Ma M, Okuyama M, Sato M, Tagami T, Klahan P, Kumagai Y, Mori H, Kimura A.

Effects of mutation of Asn694 in Aspergillus niger α-glucosidase on hydrolysis and transglucosylation.

Appl. Microbiol. Biotechnol., 101, 6399-6408 (2017)

doi:10.1007/s00253-017-8402-6. PubMed PMID: 28688044.

• Okuyama M, Miyamoto M, Matsuo I, Iwamoto S, Serizawa R, Tanuma M, Ma M, Klahan P, Kumagai Y, Tagami T, Kimura A

Substrate recognition of the catalytic α-subunit of glucosidase II from Schizosaccharomyces pombe.

Biosci. Biotechnol. Biochem., 81, 1503-1511 (2017)

doi: 10.1080/09168451.2017.1320520.  PMID: 28471318.

• Okuyama M, Matsunaga K, Watanabe KI, Yamashita K, Tagami T, Kikuchi A, Ma M, Klahan P, Mori H, Yao M, Kimura A

Efficient synthesis of α-galactosyl oligosaccharides using a mutant Bacteroides thetaiotaomicron retaining α-galactosidase (BtGH97b).

FEBS J., 284, 766-783 (2017)

doi: 10.1111/febs.14018. PubMed PMID: 28103425.

• Kikuchi A, Okuyama M, Kato K, Osaki S, Ma M, Kumagai Y, Matsunaga K, Klahan P, Tagami T, Yao M, Kimura A.

A novel glycoside hydrolase family 97 enzyme: Bifunctional β-L-arabinopyranosidase/α-galactosidase from Bacteroides thetaiotaomicron.

Biochimie, 142, 41-50 (2017)

doi:10.1016/j.biochi.2017.08.003.  PubMed PMID: 28804002.

• Suzuki N, Kim YM, Fujimoto Z, Momma M, Kang HK, Funane K, Okuyama M, Mori H, Kimura A

Crystallization and preliminary crystallographic analysis of dextranase from Streptococcus mutans, Acta Crystallogr Sect F Struct Biol Cryst Commun., 2011, 67, 1542-1544, 10.1107/S1744309111038425

• Kobayashi M, Hondoh H, Mori H, Saburi W, Okuyama M, Kimura A

Calcium ion-dependent increase in thermostability of dextran glucosidase from Streptococcus mutans, Biosci. Biotechnol. Biochem., 2011, 75 (8), 1557-1563, 10.1271/bbb.110256

• Kim YM, Shimizu R, Nakai H, Mori H, Okuyama M, Kang MS, Fujimoto Z, Funane K, Kim D, Kimura A

Truncation of N- and C-terminal regions of Streptococcus mutans dextranase enhances catalytic activity, Appl. Microbiol. Biotechnol., 2011, 91 (2), 329-339, 10.1007/s00253-011-3201-y

• Okuyama M

Function and structure studies of GH family 31 and 97 α-Glycosidases, Biosci. Biotechnol. Biochem., 2011, 75 (12), 2269-2277, 10.1271/bbb.110610

• Iwaya H, Lee JS, Yamagishi S, Shinoki A, Lang W, Thawornkuno C, Kang HK, Kumagai Y, Suzuki S, Kitamura S, Hara H, Okuyama M, Mori H, Kimura A, Ishizuka S

The delay in the development of experimental colitis from isomaltosyloligosaccharides in rats is dependent on the degree of polymerization, PLOS one, 2012, 7 (11), e50658, 10.1371/journal.pone.0050658

• Ngiwsara L, Iwai G, Tagami T, Sato N, Nakai H, Okuyama M, Mori H, Kimura A

Amino acids in conserved region II are crucial to substrate specificity, reaction velocity, and regioselectivity in the transglucosylation of honeybee GH-13 α-glucosidases, Biosci. Biotechnol. Biochem., 2012, 76 (10), 1967-1974, 10.1271/bbb.120473

• Kim YM, Yamamoto E, Kang MS, Nakai H, Saburi W, Okuyama M, Mori H, Funane K, Momma M, Fujimoto Z, Kobayashi M, Kim D, Kimura A

Bacteroides thetaiotaomicron VPI-5482 glycoside hydrolase family 66 homolog catalyzes dextranolytic and cyclization reactions, FEBS J. 2012, 279 (17), 3185-3191, 10.1111/j.1742-4658.2012.08698.x

• Kim YM, Saburi W, Yu S, Nakai H, Maneesan J, Kang MS, Chiba S, Kim D, Okuyama M, Mori H, Kimura A

A novel metabolic pathway for glucose production mediated by α-glucosidase-catalyzed conversion of 1,5-anhydrofructose, J. Biol. Chem., 2012, 287 (27), 22441-22444, 10.1074/jbc.C112.360909

• Suzuki N, Kim YM, Fujimoto Z, Momma M, Okuyama M, Mori H, Funane K, Kimura A

Structural elucidation of dextran degradation mechanism by Streptococcus mutans dextranase belonging to glycoside hydrolase family 66, J. Biol. Chem., 2012, 287 (24), 19916-19926, 10.1074/jbc.M112.342444

• Kim YM, Kiso Y, Muraki T, Kang MS, Nakai H, Saburi W, Lang W, Kang HK, Okuyama M, Mori H, Suzuki R, Funane K, Suzuki N, Momma M, Fujimoto Z, Oguma T, Kobayashi M, Kim D, Kimura A

Novel dextranase catalyzing cycloisomaltooligosaccharide formation and identification of catalytic amino acids and their functions using chemical rescue approach, J. Biol. Chem., 2012, 287 (24), 19927-19935, 10.1074/jbc.M111.339036

• Chantarudee A, Phuwapraisirisan P, Kimura K, Okuyama M, Mori H, Kimura A, Chanchao C

Chemical constituents and free radical scavenging activity of corn pollen collected from Apis mellifera hives compared to floral corn pollen at Nan, Thailand, BMC Complement. Altern. Med., 2012, 12: 45, 10.1186/1472-6882-12-45

• Teerasripreecha D, Phuwapraisirisan P, Puthong S, Kimura K, Okuyama M, Mori H, Kimura A, Chanchao C

In vitro antiproliferative/cytotoxic activity on cancer cell lines of a cardanol and a cardol enriched from Thai Apis mellifera propolis, BMC Complement. Altern. Med., 2012, 12: 27, 10.1186/1472-6882-12-27

• Song KM, Okuyama M, Kobayashi K, Mori H, Kimura A

Characterization of a glycoside hydrolase family 31 α-glucosidase involved in starch utilization in Podospora anserina, Biosci. Biotechnol. Biochem., 2013, 77(10), 2117-2124, 10.1271/bbb.130545

• Saburi W, Kobayashi M, Mori H, Okuyama M, Kimura A

Replacement of the catalytic nucleophile aspartyl residue of dextran glucosidase by cysteinesulfinate enhances transglycosylation activity, J. Biol. Chem. 2013, 288(44), 31670-31677, 10.1074/jbc.M113.491449

• Song KM, Okuyama M, Nishimura M, Tagami T, Mori H, Kimura A

Aromatic Residue on β→α loop 1 in the catalytic domain is important to the transglycosylation specificity of glycoside hydrolase family 31 α-glucosidase, Biosci. Biotechnol. Biochem., 2013, 77 (8) 1759-1765, 10.1271/bbb.130325

• Tagami T, Tanaka Y, Mori H, Okuyama M, Kimura, A

Enzymatic synthesis of acarviosyl-maltooligosaccharides using disproportionating enzyme 1, Biosci. Biotechnol. Biochem., 2013, 77 (2) 312-319, 10.1271/bbb.120732

• Shinoki A, Lang W, Thawornkuno C, Kang HK, Kumagai Y, Okuyama M, Mori H, Kimura A, Ishizuka S, Hara H

A novel mechanism for the promotion of quercetin glycoside absorption by megalo α-1,6-glucosaccharide in the rat small intestine, Food Chem., 2013, 136 (2) 293-296, 10.1016/j.foodchem.2012.08.028

• Tagami T, Okuyama M, Nakai H, Kim YM, Mori H, Taguchi K, Svensson B, Kimura A

Key aromatic residues at subsites +2 and +3 of glycoside hydrolase family 31 α-glucosidase contribute to recognition of long-chain substrates, BBA-Proteins Proteom., 2013, 1834 (1) 329-335, 10.1016/j.bbapap.2012.08.007

• Tagami T, Yamashita K, Okuyama M, Mori H, Yao M, Kimura A

Molecular basis for the recognition of long-chain substrates by plant α-glucosidases, J. Biol. Chem., 2013, 288 (26), 19296-19303, 10.1074/jbc.M113.465211

• Lang W, Sirisansaneeyakul S, Ngiwsara L, Mendes S, Martins LO, Okuyama M, Kimura A

Characterization of a new oxygen-insensitive azoreductase from Brevibacillus laterosporus TISTR1911: Towards dye decolorization using packed-bed metal affinity reactor, Bioresour. Technol. 2013, 150, 298-306, 10.1016/j.biortech.2013.09.124.

• 奥山　正幸

糖質加水分解酵素ファミリー31に属するα-グルコシダーゼの構造と基質・反応特異性, バイオサイエンスとインダストリー, 2013, 71 (5), 424-428

• Suzuki N, Kim YM, Momma M, Fujimoto Z, Kobayashi M, Kimura A, Funane K

Crystallization and preliminary X-ray crystallographic analysis of cycloisomaltooligosaccharide glucanotransferase from Bacillus circulans T-3040, Acta Crystallogr Sect F Struct Biol Cryst Commun., 2013, 69(Pt 8), 946-949, 10.1107/S174430911301991X

• Lang W, Kumagai Y, Sadahiro J, Maneesan J, Okuyama M, Mori H, Sakairi N, Kimura A

Different molecular complexity of linear-isomaltomegalosaccharides and β-cyclodextrin on enhancing solubility of azo dye ethyl red: Towards dye biodegradation, Bioresource Technol., 2014, 169, 518-524, 10.1016/j.biortech.2014.07.025

• Okuyama M, Yoshida T, Hondoh H, Mori H, Yao M, Kimura A

Catalytic role of the calcium ion in GH97 inverting glycoside hydrolase, FEBS Lett., 2014, 588 (17), 3213-3217, 10.1016/j.febslet.2014.07.002

• Lang W, Sirisansaneeyakul S, Martins, L O, Ngiwsara L, Sakairi N, Pathom-aree W, Okuyama M, Mori H, Kimura, A

Biodecolorization of a food azo dye by the deep sea Dermacoccus abyssi MT1.1(T) strain from the Mariana Trench, J. Environ. Manage., 2014, 132, 155-164, 10.1016/j.jenvman.2013.11.002

• Maneesan J, Matsuura H, Tagami T, Mori H, Kimura A

Production of 1,5-anhydro-d-fructose by an α-glucosidase belonging to glycoside hydrolase family 31. Biosci. Biotechnol. Biochem., 2014, 78(12), 2064-2068, 10.1080/09168451.2014.943651.

• Suzuki N, Fujimoto Z, Kim YM, Momma M, Kishine N, Suzuki R, Suzuki S, Kitamura S, Kobayashi M, Kimura A, Funane K.

Structural elucidation of the cyclization mechanism of α-1,6-glucan by Bacillus circulans T-3040 cycloisomaltooligosaccharide glucanotransferase, J. Biol. Chem. 2014, 289(17), 12040-12051, 10.1074/jbc.M114.547992

• Funane K, Ichinose H, Araki M, Suzuki R, Kimura K, Fujimoto Z, Kobayashi M, Kimura A

Evidence for cycloisomaltooligosaccharide production from starch by Bacillus circulans T-3040, Appl. Microbiol. Biotechnol., 2014, 98(9), 3947-3954, 10.1007/s00253-014-5515-z

• 木村 淳夫

糖質酵素の分子機構に関する研究 (受賞論文)、応用糖質科学 : 日本応用糖質科学会誌, 2014, 4(1), 3-16

• 田上 貴祥

GH31 α-グルコシダーゼの基質特異性と構造の多様性：基質重合度に対する特異性を決める局所構造

化学と生物, 52, 205-207 (2014)

• Kumagai Y, Yamashita K, Tagami T, Uraji M, Wan K, Okuyama M, Yao M, Kimura A, Hatanaka T

The loop structure of Actinomycete glycoside hydrolase family 5 mannanases governs substrate recognition

FEBS J. 2015, 10.1111/febs.13401

• Suzuki R, Suzuki N, Fujimoto Z, Momma M, Kimura K, Kitamura S, Kimura A, Funane K

Molecular engineering of cycloisomaltooligosaccharide glucanotransferase from Bacillus circulans T-3040: structural determinants for the reaction product size and reactivity, Biochem. J. 2015 [Epub ahead of print]

• Saburi W, Rachi-Otsuka H, Hondoh H, Okuyama M, Mori H, Kimura A

Structural Elements Responsible for the Glucosidic Linkage-selectivity of a Glycoside Hydrolase Family 13 Exo-glucosidase,FEBS Lett., 2015, 10.1016/j.febslet.2015.02.023

• 奥山　正幸

糖質関連酵素の最近の進歩(4) 糖質加水分解酵素ファミリー内の機能の保存性と多様性, 化学と生物: 日本農芸化学会会誌: 生命・食・環境, 2015, 53 (2), 120-126

• Kobayashi M, Saburi W, Nakatsuka D, Hondoh H, Kato K, Okuyama M, Mori H, Kimura A, Yao M

Structural insights into the catalytic reaction that is involved in the reorientation of Trp238 at the substrate-binding site in GH13 dextran glucosidase, FEBS Lett., 2015, 589 (4), 484-489, 10.1016/j.febslet.2015.01.005

• Tagami T, Yamashita K, Okuyama M, Mori H, Yao M, Kimura A

Structural advantage of sugar beet α-glucosidase to stabilize the Michaelis complex with long-chain substrate, J. Biol. Chem., 2015, 290 (3), 1796-1803, 10.1074/jbc.M114.606939

• Sadahiro J, Mori H, Saburi W, Okuyama M, Kimura A

Extracellular and cell-associated forms of Gluconobacter oxydans dextran dextrinase change their localization depending on the cell growth, Biochem. Biophys. Res. Commun., 2015, 456 (1), 500-505, 10.1016/j.bbrc.2014.11.115

• Saburi W, Okuyama M, Kumagai Y, Kimura A, Mori H

Biochemical properties and substrate recognition mechanism of GH31 α-glucosidase from Bacillus sp. AHU 2001 with broad substrate specificity, Biochimie, 2015, 108, 140-148, 10.1016/j.biochi.2014.11.010.