Name: Rhyland O'Neill
Internship Site: Georgia Institute of Technology - Walter H Coutler Department of Biomedical Engineering
Mentor: Daniel Shah, BotchweyLab
Dates of Internship: Summer 2024 - Now
Overview: During my internship at Project ENGAGES, I have worked on a research project focused on reducing endothelial cell activation in sickle cell disease (SCD) patients using microRNA-loaded extracellular vesicles (EVs). My responsibilities have mainly included isolating and characterizing EVs from red blood cells of different genotypes (AA, AS, SS), analyzing size and concentration data, and assisting with experimental design while also presenting to many different audiences. The environment is collaborative and research-driven, with exposure to advanced lab techniques and data analysis tools. There are also opportunities for professional development through workshops and conferences such as NSBE.
Above: Rhyland presents at the Project ENGAGES Summer Symposium.
Purpose of Internship Site: Project ENGAGES' focus is to expose minority students to undergraduate and professional research opportunities in STEM to provide an in-depth exposure to science. The Georgia Tech Biomedical Engineering Department exists to advance healthcare solutions through engineering principles, focusing on medical devices, cellular therapies, and disease modeling. The BotchweyLab aims to develop innovative approaches to regenerative engineering of damaged tissues and organs.
Type of Work Done: The lab conducts experimental research involving immune cell therapies, lipid-based biomaterials, and bioactive lipidomics. Projects explore the intersections between biology, engineering, and computational methods.
Mission/Goals of Department/Team: Our team’s goal is to blend the power of the immune system, the versatility of advanced materials, and the intricacy of lipid signaling to pioneer new frontiers in biomaterials and regenerative medicine.
Tools, Software, and Equipment:
The tools that I use while in the lab are:
Laboratory Equipment: Ultracentrifuge, nanoparticle tracking analyzer (NTA), pipettes, biosafety cabinet
Software: ImageJ for microscopy analysis, Excel for data logging, GraphPad Prism for statistical analysis
Techniques: EV isolation, size characterization, miRNA loading
Above: Nanoparticle Tracking Analysis in action!
Above: Rhyland takes the isolated EV Pellet and prepares it for another ultracentrifugation step.
Project Description: My main project aimed to determine whether EVs from Sickle Cell Disease (SCD) patients could be loaded with microRNAs to reduce endothelial activation. This matters because endothelial activation contributes to painful vaso-occlusive crises in SCD, and targeting this pathway could improve patient outcomes and reduce inflammation (pain).
My Role: I developed experimental plans and implemented them in my research. One example includes isolating EVs from AA, AS, and SS genotype samples and measured their size and concentration using NTA. I also analyzed data using GraphPad Prism and Microsoft Excel.
Process + Engineering Methods:
Steps Taken:
Collected RBC samples from different genotypes.
Isolated EVs using differential centrifugation and filtration.
Characterized EVs using nanoparticle tracking analysis (size and concentration).
Methods Used:
Data Analysis: Statistical comparison of EV size and concentration across genotypes
Engineering Framework: Experimental design and optimization for EV isolation
Challenges:
One challenge was obtaining consistent EV yields from SS samples due to their fragility. We solved this by adjusting centrifugation speeds and filtration steps.
Results + Impact:
We found that SS genotype produced smaller and more EVs compared to AA and AS genotypes, which could influence therapeutic loading efficiency. My work contributed to understanding genotype-specific EV characteristics, informing future therapeutic strategies.
Learning: I gained insight into how engineering principles apply to biological systems and the importance of precision in experimental design.
Above: Fractionating Blood in under the Tissue Culture Hood.
Above: Diving through research papers to develop an experimental protocol.
Above: Posing by my poster at the 2026 Atlanta City Regional Science Fair!
Above: All smiles after qualifying for ISEF 2025!
The following skills are described in the same order of the corresponding images:
Data Analysis: Used GraphPad Prism to analyze EV size distributions and perform statistical tests.
Laboratory Techniques: Mastered Cell Culture and EV isolation process using ultracentrifugation and filtration.
Communication: Presented findings at science fairs, at Project ENGAGES poster sessions, and during lab meetings and collaborated with graduate researchers.
Project Management: Maintained detailed logs of experiments and results for reproducibility, developed detailed reports, posters, and presentations based on experiments and data
Above: Moments before the ISEF judging begins!
1. What was the most important thing you learned from your internship?
The most important thing I learned was how critical it is to be able to present your work to others clearly and confidently. In research and engineering, doing great work is only part of the equation; you also need to communicate your findings so others can understand and build on them. During my internship, I had opportunities to share results in lab meetings, poster sessions, and science fairs, and explain complex concepts to people who weren’t familiar with the details. This taught me how to simplify technical language without losing accuracy, which is a valuable skill for any engineer. I realized that strong presentation skills can influence how your work is perceived and even open doors for collaboration. Being able to present effectively is just as important as running experiments because it ensures your contributions have impact beyond the lab.
2. What engineering practices or mindsets did you see professionals using?
I observed professionals using the engineering design process extensively, even in a research setting. They approached problems systematically: defining the issue, brainstorming solutions, testing hypotheses, and refining methods based on data. Another mindset I noticed was adaptability. Experiments rarely go exactly as planned, so being flexible and creative in troubleshooting is key. Professionals also have a huge emphasis on documentation and reproducibility, ensuring that every step is recorded for future reference and credibility. Collaboration is another major practice; team members regularly share updates and seek feedback to improve outcomes. Finally, I saw a strong commitment to ethical responsibility, especially when working with biological samples, which highlighted the importance of integrity in engineering work.
3. What surprised you about the engineering workplace?
I was surprised by how interdisciplinary the engineering workplace is. I expected to work mostly with engineers, but I interact with biologists, chemists, and doctors daily. This showed me that solving complex problems often requires expertise from multiple fields. Another surprise was the level of creativity involved in research. Engineering isn’t just technical; it requires innovative thinking to design experiments and interpret results, which takes a lot of background knowledge. I also didn’t expect how much time is spent troubleshooting and refining processes compared to running actual tests. The collaborative culture was another pleasant surprise; everyone is willing to help and share knowledge even in other laboratories, which makes the environment feel supportive. Overall, the workplace is dynamic and intellectually stimulating, which has challenged me to think beyond traditional engineering concepts.
4. How did this internship influence your future goals?
Project ENGAGES has solidified my interest in biomedical engineering, sports medicine, and global health. Before, I was considering several STEM fields, but now I feel confident that I want to pursue research that directly impacts patient care. Seeing how engineering can improve treatments for diseases like sickle cell disease has inspired me to focus on health equity and innovation. It has also motivated me to seek opportunities that combine engineering with biology and data science, as these intersections are where breakthroughs happen. Additionally, the experience has taught me the importance of graduate-level research, so I plan to consider pursuing advanced degrees to deepen my expertise. Ultimately, this internship has given me clarity and direction for my academic and career path.
5. What advice would you give a future student in this internship?
My biggest advice is to be proactive and curious. Don’t be afraid to ask questions, even small ones, because understanding the “why” behind each step will make your experience have purpose and meaning. Take detailed notes during experiments; documentation is critical in research and will help you track progress. Also, be prepared for challenges and setbacks. Experiments often fail before they succeed, so persistence is key. Build relationships with your mentors and peers; networking and collaboration can open doors to future opportunities. Finally, approach the internship with an open mind and a willingness to learn, because the skills and insights you gain will go far beyond the lab.
I presented during STEAM Career Day to share my internship experience with middle school and elementary school students at Drew Charter, explaining the role of biomedical engineering in addressing sickle cell disease. As a result of this, students gained insight on how the engineering pathway led me to becoming a Project ENGAGES scholar. I hope that I was able to inspire students to take charge of their own path.