Practicals

🧪Experiments going to be conducted/conducting (2025-26) 

Ex. No. 1 -  Date 20-02-2026 -  Catalase and Oxidase activity 

For a screen cast video based explanation click HERE; 

(i) To understand about the concept of Catalase click HERE and to know about Oxidase click HERE

Ex. No. 2 -  Date 27-02-2026 -  Urease activity

Ex. No. 3 -  Date 13-03-2026 -  Citrate utilization

Ex. No. 4 -  Date 10-04-2026 -  Nitrate reduction and denitrification

 (i) The theoretical background of the experiment can be understood by clicking HERE (ii) To understand the concept click HERE to watch a video prepared by students (201-2022 batch)

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Ex. No. 5 -  Date 16-09-2021 -  Estimation of fungal biomass by gravimetric analysis 

Ex. No. 6.-  Date 21-10-2021 -  Effect of pH on the growth of bacteria

Ex. No. 7 -  Date 11-11-2021 -  Assessing the indole, hydrogen sulfide production and motility of bacteria using SIM agar

MODEL QUESTIONS FOR - PRACTICAL INTERNAL TEST

Practical skills to learn 

1. To know how to prepare a cotton plug click HERE and HERE

2. To know how to make a quadrant streaking on a petri plate containing agar click HERE

Keep the following points in mind while preparing and submitting record for valuation

1.  Maintain a 80 pages unruled long size notebook as OBSERVATION NOTE

2. Write in the RECORD NOTE BOOK similar to one you use in schools and should NOT write in loose sheets and bind.

3. Fill the first paragraph of the certificate page, paste in the record, click HERE to download the certificate page.  

4. Fill the index page with the details of the Expt No., title, date and submit.

5. Scientific names need to be underlined; check HERE to know how to write scientific names

6. While writing the experiment in right side pages of a record, dont forget to add in the top left side corner the experiment number and date. 

7. While writing experiment remember, diagrams (draw with pencil)  media composition/ equations are need to be written in left side and the textual portions alone need to be written in the right side. 

8. If you plan to submit late due to any personal reasons or issues, before submission date get permission from course teacher by filling the permission form.

9. Cover the record with a brown cover and submit. 

NEP 2023-24 Syllabus

MJD5                                                              BACTERIAL PHYSIOLOGY AND METABOLISM                                               (3+1 credit)

Syllabus:

1. Determination of growth curve of bacteria.

2. Bacterial population count by turbidity method. 

3. Estimation of fungal growth by gravimetric estimation.

4. Effect of temperature on bacterial growth.

5. Effect of pH on bacterial growth.

6. Biochemical characterization of bacterial cultures: catalase, urease, cytochrome oxidase and sugar fermentation, citrate utilization,  gelatin liquefaction, sulfide indole motility test.

7. Nitrate reduction and denitrification by bacteria.

8. Biolog/API based on biochemical tests (demonstration).

Practical manual

Download the biochemical experiments from the below link 

      Cappuccino J.G. and Sherman N. (2014) Microbiology a Laboratory Manual. 10th Edn., Pearson Education p.567

Topics may be covered from the 

Ministry of Education, India,

Under the National Mission on Education through ICT

Microbiology virtual Lab-1 Catalase and Coagulase Test, Lecithinase test, Bacterial growth curve, carbohydrate fermentation test, Antibiotic susceptibility testing 

Microbiology Virtual Lab-2  Urease test, Litmus Milk test, Triple sugar iron agar.

    Growth Curves: Generating Growth Curves Using Colony Forming Units and Optical Density Measurements - JoVe webpage based teaching content  

Archives 

Practical tasks submission

1. Fill the FORM related to your willingness to take up the Urease and Citrate utilization test 

2. The steps in the performance (those who opted individually to do the experiment) of Nitrate reduction & denitrification was provided HERE

3. Fill this FORM related to your willingness to take up the Nitrate reduction & Denitrification practical individually or in group (last date to submit - 11-10-2021, 11.59 pm)

4. To submit the copy of the Streak plate you prepared by click HERE (last date to submit - 20-9-2021, 11.59 pm)

5. To fix the date on which you are plan to take up cotton plug making click HERE  (last date to fix - 21-9-2021, 11.59 pm)

6. To perform the inperson streaking of plates (as wet lab) click HERE