Introduction

This information was originally compiled in a printed manual originally written in 1999 to provide a compendium of up-to-date commonly used protocols that my research students use in my protein engineering laboratory at Colgate University. It was moved on an online format in 2007. Much of the information contained in this manual was gleaned from my knowledgeable, kind, and very clever colleagues in the laboratory of Dr. Edith Wilson Miles in the National Institute of Diabetes and Digestive and Kidney Diseases at the National Institutes of Health. Special thanks goes to Dr. Li-hong Yang, my very patient molecular biology and recombinant DNA mentor. I am forever in debt to his expert tutelage. Additional thanks go to the many Colgate undergraduates who have suggested and trialed new (to us) protein engineering methods in the research laboratory.