Embedded Files
Epigenetic memory and inheritance
DNA methylation and histone modifications are remodeled extensively during early mammalian embryogenesis, leading to the establishment of gene expression programs that cells maintain heritably through cell divisions and differentiation.
We seek to understand the interplay between DNA methylation and histone modifications that enable epigenetic memory and inheritance, particularly during complex cellular differentiation processes. Additionally, we employ genetics and proteomics to discover the factors that mediate the establishment and spreading of epigenetic modifications on the human genome.
CRISPR-based epigenome editing
We engineer CRISPR epigenome editing technologies for turning off/on genes by fusing catalytically dead Cas9 to epigenetic modifiers. For example, our CRISPRoff/on technologies enable long-term epigenetic modulation of genes by editing DNA methylation and histone modifications of mammalian genes.
We apply our technologies for functional genomics and targeted modulation of gene expression in human cells. Our tools enable fine-tuned programming of gene expression for cell and tissue engineering and as potential vivo therapeutic modalities.
DNA methylation in health and disease
DNA methylation is the most abundant epigenetic modification on the human genome and regulates gene expression, genomic imprinting, and silencing transposable elements.Â
We study how DNA methylation is regulated in human health and altered in disease. We bridge systems-wide, genetics, and biochemistry to tackle our research questions.
Page updated
Report abuse