Select Publications
2022
Fluorescent Reporters for Sensing Membrane Potential: Tools for Bioelectricity. Kramer RH, Miller EW, Abdelfattah A, Baker B. Bioelectricity. DOI: 10.1089/bioe.2022.0017
Interrogating the function of GABAA receptors in the brain with optogenetic pharmacology. Kramer RH, Rajappa R. Current Opinion in Pharmacology. DOI: 10.1016/j.coph.2022.102198
Retinoic acid inhibitors mitigate vision loss in a mouse model of retinal degeneration. Telias M, Sit K, Frozenfar D, Smith B, Misra A, Goard MJ, Kramer RH. Science Advances. DOI: 10.1126/sciadv.abm4643
2021
Cyclodextrin-Assisted Delivery of Azobenzene Photoswitches for Uniform and Long-Term Restoration of Light Responses in Degenerated Retinas of Blind Mice. Cao KJ, Lyons EF, Smith BE, Denlinger BL, Ma H, Shirian JD, Kramer RH. Advanced Therapeutics. DOI: 10.1002/adtp.202100127
Voltage Imaging with a NIR-Absorbing Phosphine Oxide Rhodamine Voltage Reporter. Gonzalez MA, Walker AS, Cao KJ, Lazzari-Dean JR, Settineri NS, Kong EJ, Kramer RH, Miller EW. Journal of the American Chemical Society. DOI: 10.1021/jacs.0c11382
Relocation of an extrasynaptic GABAA receptor to inhibitory synapses freezes excitatory synaptic strength and preserves memory. Davenport C*, Rajappa R*, Katchan L, Taylor CR, Tsai MT, Smith CM, de Jong J, Arnold DB, Lammel S, Kramer RH. Neuron. DOI: 10.1016/j.neuron.2020.09.037. PDF. (highlighted in Nat. Neurosci.)
A potential common link between glial cells, calcium changes, modulation of synaptic transmission, spreading depression, migraine and epilepsy - H+. Malchow RP, Tchernookova BK, Cho JV, Smith PJS, Kramer RH, and Kreitzer MA. Frontiers in Cellular Neuroscience. DOI: 10.3389/fncel.2021.693095
Evaluating methods and protocols of ferritin-based magnetogenetics. Hernandez-Morales R, Han V, Kramer RH, and Liu C. iScience. DOI: 10.1016/j.isci.2021.103094
2020
Degeneration-Dependent Retinal Remodeling: Looking for the Molecular Trigger. Telias M, Nawy S, Kramer RH. Frontiers in Neuroscience. DOI: 10.3389/fnins.2020.618019
Controlling Horizontal Cell-Mediated Lateral Inhibition in Transgenic Zebrafish Retina with Chemogenetic Tools. Beckwith-Cohen B, Holzhausen LC, Nawy S, Kramer RH. N. eNeuro. DOI: 10.1523/ENEURO.0022-20.2020
Parvalbumin interneurons provide spillover to newborn and mature dentate granule cells. Vaden RJ, Gonzalez JC, Tsai MC, Niver AJ, Griffith CJ, Kramer RH, Wadiche JI, Overstreet-Wadiche L. eLife. DOI: 10.7554/eLife.54125
2019
Local photoreceptor degeneration causes local pathophysiological remodeling of retinal neurons. Denlinger B, Helft Z, Telias M, Lorach H, Palanker D, and Kramer RH. JCI Insight. DOI: 10.1172/jci.insight.132114
Retinoic acid is the trigger for neural hyperactivity in retinal degeneration and blocking its receptor unmasks light responses and augments vision. Telias M*, Denlinger B*, Helft Z*, Beckwith-Cohen B, Thornton C, and Kramer RH. Neuron. DOI: 10.1016/j.neuron.2019.02.015
Localizing proton-mediated inhibitory feedback at the retinal horizontal cell-cone synapse with genetically-encoded pH probes. Beckwith-Cohen B, Holzhausen LC, Wang TM, Rajappa R, Kramer RH. Journal of Neuroscience. DOI: https://doi.org/10.1523/JNEUROSCI.1541-18.2018
2018
Manipulating midbrain dopamine neurons and reward-related behaviors with light-controllable nicotinic acetylcholine receptors. Durand-de Cuttoli R, Mondoloni S, Marti F, Lemoine D, Nguyen C, Naudé J, d'Izarny-Gargas T, Pons S, Maskos U, Trauner D, Kramer RH, Faure P, Mourot A. eLife. DOI: 10.7554/eLife.37487
Design of a Highly Bistable Photoswitchable Tethered Ligand for Rapid and Sustained Manipulation of Neurotransmission. Lin WC, Tsai MC, Rajappa R, Kramer RH. Journal of the American Chemical Society. DOI: 10.1021/jacs.8b03942
BRestoring Vision to the Blind with Chemical Photoswitches. Tochitsky I, Kienzler MA, Isacoff E, Kramer RH. Chemical Reviews. DOI: 10.1021/acs.chemrev.7b00723
Restoration of patterned vision with an engineered photoactivatable G protein-coupled receptor. Berry MH, Holt A, Levitz J, Broichhagen J, Gaub BM, Visel M, Stanley C, Aghi K, Kim YJ, Cao K, Kramer RH, Trauner D, Flannery J, Isacoff EY. Nature Communications. DOI: 10.1038/s41467-018-03585-2
Light-Switchable Ion Channels and Receptors for Optogenetic Interrogation of Neuronal Signaling Lin WC, Kramer RH Bioconjugate Chemistry. DOI: 10.1021/acs.bioconjchem.7b00803
2017
Understanding and improving photo-control of ion channels in nociceptors with azobenzene photoswitches. Mourot A, Herold C, Kienzler MA, Kramer RH. British Journal of Pharmacology. DOI: 10.1111/bph.13923
Photopharmacological control of bipolar cells restores visual function in blind mice. Laprell L, Tochitsky I, Kaur K, Manookin MB, Stein M, Barber DM, Schön C, Michalakis S, Biel M, Kramer RH, Sumser MP, Trauner D, Van Gelder RN. The Journal of Clinical Investigation. DOI: 10.1172/JCI92156
Restoring visual function to the blind retina with a potent, safe and long-lasting photoswitch. Tochitsky I, Trautman J, Gallerani N, Malis JG, Kramer RH. Scientific Reports. DOI: 10.1038/srep45487
2016
How Azobenzene Photoswitches Restore Visual Responses to the Blind Retina. Tochitsky I, Helft Z, Meseguer V, Fletcher RB, Vessey KA, Telias M, Denlinger B, Malis J, Fletcher EL, Kramer RH. Neuron. DOI: 10.1016/j.neuron.2016.08.038
Serotonin modulates spike probability in the axon initial segment through HCN channels. Ko KW, Rasband MN, Meseguer V, Kramer RH, Golding NL. Nature Neuroscience. DOI: 10.1038/nn.4293
2015
Lateral Inhibition in the Vertebrate Retina: The Case of the Missing Neurotransmitter. Kramer RH, Davenport CM. PLOS Biology. DOI: 10.1371/journal.pbio.1002322
A Comprehensive Optogenetic Pharmacology Toolkit for In Vivo Control of GABAA Receptors and Synaptic Inhibition. Lin WC, Tsai MC, Davenport CM, Smith CM, Veit J, Wilson NM, Adesnik H, Kramer RH. Neuron. DOI: 10.1016/j.neuron.2015.10.026
Optopharmacological tools for restoring visual function in degenerative retinal diseases. Tochitsky I, Kramer RH. Current Opinion in Neurobiology. DOI: 10.1016/j.conb.2015.01.018
2014
Engineering a light-regulated GABAa receptor for optical control of neural inhibition. Lin WC, Davenport CM, Mourot A, Vytla D, Smith CM, Medeiros KA, Chambers JJ, Kramer RH. ACS Chemical Biology. DOI: 10.1021/cb500167u
Restoring visual function to blind mice with a photoswitch that exploits electrophysiological remodeling of retinal ganglion cells. Tochitsky I, Polosukhina A, Degtyar VE, Gallerani N, Smith CM, Friedman A, Van Gelder RN, Trauner D, Kaufer D, Kramer RH. Neuron. DOI: 10.1016/j.neuron.2014.01.003
Imaging an optogenetic pH sensor reveals that protons mediate lateral inhibition in the retina. Wang TM, Holzhausen LC, Kramer RH. Nature Neuroscience. DOI: 10.1038/nn.3627
2013
Optogenetic pharmacology for control of native neuronal signaling proteins. Kramer RH, Mourot A, Adesnik H. Nature Neuroscience. DOI: 10.1038/nn.3424
Light at the end of the channel: optical manipulation of intrinsic neuronal excitability with chemical photoswitches. Mourot A, Tochitsky I, Kramer RH. Frontiers in Molecular Neuroscience. DOI: 10.3389/fnmol.2013.00005
Photochromic potassium channel blockers: design and electrophysiological characterization. Mourot A, Fehrentz T, Kramer RH. Methods in Molecular Biology. DOI: 10.1007/978-1-62703-345-9_7
2012
Photochemical restoration of visual responses in blind mice. Polosukhina A, Litt J, Tochitsky I, Nemargut J, Sychev Y, De Kouchkovsky I, Huang T, Borges K, Trauner D, Van Gelder RN, Kramer RH. Neuron. DOI: 10.1016/j.neuron.2012.05.022
Optical control of endogenous proteins with a photoswitchable conditional subunit reveals a role for TREK1 in GABA(B) signaling. Sandoz G, Levitz J, Kramer RH, Isacoff EY. Neuron. DOI: 10.1016/j.neuron.2012.04.026
Rab3A mediates vesicle delivery at photoreceptor ribbon synapses. Tian M, Xu CS, Montpetit R, Kramer RH. The Journal of Neuroscience. DOI: 10.1523/JNEUROSCI.0265-12.2012
Rapid optical control of nociception with an ion-channel photoswitch. Mourot A, Fehrentz T, Le Feuvre Y, Smith CM, Herold C, Dalkara D, Nagy F, Trauner D, Kramer RH. Nature Methods. DOI: 10.1038/nmeth.1897
Optochemical control of genetically engineered neuronal nicotinic acetylcholine receptors. Tochitsky I, Banghart MR, Mourot A, Yao JZ, Gaub B, Kramer RH, Trauner D. Nature Chemistry. DOI: 10.1038/nchem.1234
Water-soluble, donor-acceptor biphenyl derivatives in the 2-(o-nitrophenyl)propyl series: highly efficient two-photon uncaging of the neurotransmitter γ-aminobutyric acid at λ = 800 nm. Donato L, Mourot A, Davenport CM, Herbivo C, Warther D, Léonard J, Bolze F, Nicoud JF, Kramer RH, Goeldner M, Specht A. Angewandte Chemie. DOI: 10.1002/anie.201106559
2011
Tuning photochromic ion channel blockers. Mourot A, Kienzler MA, Banghart MR, Fehrentz T, Huber FM, Stein M, Kramer RH, Trauner D. ACS Chemical Neuroscience. DOI: 10.1021/cn200037p
Release from the cone ribbon synapse under bright light conditions can be controlled by the opening of only a few Ca(2+) channels. Bartoletti TM, Jackman SL, Babai N, Mercer AJ, Kramer RH, Thoreson WB. Journal of Neurophysiology. DOI: 10.1152/jn.00634.2011
Engineering light-regulated ion channels. Fortin DL, Dunn TW, Kramer RH. Cold Spring Harbor Protocols. DOI: 10.1101/pdb.top112
LiGluR restores visual responses in rodent models of inherited blindness. Caporale N, Kolstad KD, Lee T, Tochitsky I, Dalkara D, Trauner D, Kramer R, Dan Y, Isacoff EY, Flannery JG. Molecular Therapy. DOI: 10.1038/mt.2011.103
A positive feedback synapse from retinal horizontal cells to cone photoreceptors. Jackman SL, Babai N, Chambers JJ, Thoreson WB, Kramer RH. Plos Biology. DOI: 10.1371/journal.pbio.1001057
Optogenetic photochemical control of designer K+ channels in mammalian neurons. Fortin DL, Dunn TW, Fedorchak A, Allen D, Montpetit R, Banghart MR, Trauner D, Adelman JP, Kramer RH. Journal of Neurophysiology. DOI: 10.1152/jn.00251.2011
2010 and earlier
Photochromic blockers of voltage-gated potassium channels. Banghart MR, Mourot A, Fortin DL, Yao JZ, Kramer RH, Trauner D. Angewandte Chemie. DOI: 10.1002/anie.200904504
New photochemical tools for controlling neuronal activity. Kramer RH, Fortin DL, Trauner D. Current Opinion in Neurobiology. DOI: 10.1016/j.conb.2009.09.004
Role of the synaptic ribbon in transmitting the cone light response. Jackman SL, Choi SY, Thoreson WB, Rabl K, Bartoletti TM, Kramer RH. Nature Neuroscience. DOI: 10.1038/nn.2267
Light-activated ion channels for remote control of neural activity. Chambers JJ, Kramer RH. Methods in Cell Biology. DOI: 10.1016/S0091-679X(08)00811-X
Photochemical control of endogenous ion channels and cellular excitability. Fortin DL, Banghart MR, Dunn TW, Borges K, Wagenaar DA, Gaudry Q, Karakossian MH, Otis TS, Kristan WB, Trauner D, Kramer RH. Nature Methods. 5: 331-8. DOI: 10.1038/nmeth.1187
Staples, tape measures, and bungee cords: a variety of bifunctional reagents for understanding and controlling ion channels. Mourot A, Kramer RH. ACS Chemical Biology. DOI: 10.1021/cb700135a
Remote control of neuronal activity with a light-gated glutamate receptor. Szobota S, Gorostiza P, Del Bene F, Wyart C, Fortin DL, Kolstad KD, Tulyathan O, Volgraf M, Numano R, Aaron HL, Scott EK, Kramer RH, Flannery J, Baier H, Trauner D, Isacoff EH. Neuron. DOI: 10.1016/j.neuron.2007.05.010
Synaptic Ca2+ in darkness is lower in rods than cones, causing slower tonic release of vesicles. Sheng Z, Choi SY, Dharia A, Li J, Sterling P, Kramer RH. The Journal of Neuroscience. DOI: 10.1523/JNEUROSCI.5386-06.2007
Light-induced depolarization of neurons using a modified Shaker K(+) channel and a molecular photoswitch. Chambers JJ, Banghart MR, Trauner D, Kramer RH. Journal of Neurophysiology. DOI: 10.1152/jn.00318.2006
Allosteric control of an ionotropic glutamate receptor with an optical switch. Volgraf M, Gorostiza P, Numano R, Kramer RH, Isacoff EY, Trauner D. Nature Chemical Biology. DOI: 10.1038/nchembio756
2005 and earlier
Photochemical tools for remote control of ion channels in excitable cells. Kramer RH, Chambers JJ, Trauner D. Nature Chemical Biology. DOI:10.1038/nchembio750L3
Encoding light intensity by the cone photoreceptor synapse. Choi SY, Borghuis BG, Borghuis B, Rea R, Levitan ES, Sterling P, Kramer RH. Neuron. DOI: 10.1016/j.neuron.2005.09.011
Imaging light-modulated release of synaptic vesicles in the intact retina: retinal physiology at the dawn of the post-electrode era. Choi SY, Sheng Z, Kramer RH. Vision Research. DOI: 10.1016/j.visres.2005.08.011
Light-activated ion channels for remote control of neuronal firing. Banghart M, Borges K, Isacoff E, Trauner D, Kramer RH. Nature Neuroscience. DOI: 10.1038/nn1356