Select Publications

2022

Fluorescent Reporters for Sensing Membrane Potential: Tools for Bioelectricity. Kramer RH, Miller EW, Abdelfattah A, Baker B. Bioelectricity. DOI: 10.1089/bioe.2022.0017

Interrogating the function of GABAA receptors in the brain with optogenetic pharmacology. Kramer RH, Rajappa R. Current Opinion in Pharmacology. DOI: 10.1016/j.coph.2022.102198

Retinoic acid inhibitors mitigate vision loss in a mouse model of retinal degeneration. Telias M, Sit K, Frozenfar D, Smith B, Misra A, Goard MJ, Kramer RH. Science Advances. DOI: 10.1126/sciadv.abm4643

2021

Cyclodextrin-Assisted Delivery of Azobenzene Photoswitches for Uniform and Long-Term Restoration of Light Responses in Degenerated Retinas of Blind Mice. Cao KJ, Lyons EF, Smith BE, Denlinger BL, Ma H, Shirian JD, Kramer RH. Advanced Therapeutics. DOI: 10.1002/adtp.202100127

Voltage Imaging with a NIR-Absorbing Phosphine Oxide Rhodamine Voltage Reporter. Gonzalez MA, Walker AS, Cao KJ, Lazzari-Dean JR, Settineri NS, Kong EJ, Kramer RH, Miller EW. Journal of the American Chemical Society. DOI: 10.1021/jacs.0c11382

Relocation of an extrasynaptic GABAA receptor to inhibitory synapses freezes excitatory synaptic strength and preserves memory. Davenport C*, Rajappa R*, Katchan L, Taylor CR, Tsai MT, Smith CM, de Jong J, Arnold DB, Lammel S, Kramer RH. Neuron. DOI: 10.1016/j.neuron.2020.09.037. PDF. (highlighted in Nat. Neurosci.)

A potential common link between glial cells, calcium changes, modulation of synaptic transmission, spreading depression, migraine and epilepsy - H+. Malchow RP, Tchernookova BK, Cho JV, Smith PJS, Kramer RH, and Kreitzer MA. Frontiers in Cellular Neuroscience. DOI: 10.3389/fncel.2021.693095

Evaluating methods and protocols of ferritin-based magnetogenetics. Hernandez-Morales R, Han V, Kramer RH, and Liu C. iScience. DOI: 10.1016/j.isci.2021.103094

2020

Degeneration-Dependent Retinal Remodeling: Looking for the Molecular Trigger. Telias M, Nawy S, Kramer RH. Frontiers in Neuroscience. DOI: 10.3389/fnins.2020.618019

Controlling Horizontal Cell-Mediated Lateral Inhibition in Transgenic Zebrafish Retina with Chemogenetic Tools. Beckwith-Cohen B, Holzhausen LC, Nawy S, Kramer RH. N. eNeuro. DOI: 10.1523/ENEURO.0022-20.2020

Parvalbumin interneurons provide spillover to newborn and mature dentate granule cells. Vaden RJ, Gonzalez JC, Tsai MC, Niver AJ, Griffith CJ, Kramer RH, Wadiche JI, Overstreet-Wadiche L. eLife. DOI: 10.7554/eLife.54125

2019

Local photoreceptor degeneration causes local pathophysiological remodeling of retinal neurons. Denlinger B, Helft Z, Telias M, Lorach H, Palanker D, and Kramer RH. JCI Insight. DOI: 10.1172/jci.insight.132114

Retinoic acid is the trigger for neural hyperactivity in retinal degeneration and blocking its receptor unmasks light responses and augments vision. Telias M*, Denlinger B*, Helft Z*, Beckwith-Cohen B, Thornton C, and Kramer RH. Neuron. DOI: 10.1016/j.neuron.2019.02.015

Localizing proton-mediated inhibitory feedback at the retinal horizontal cell-cone synapse with genetically-encoded pH probes. Beckwith-Cohen B, Holzhausen LC, Wang TM, Rajappa R, Kramer RH. Journal of Neuroscience. DOI: https://doi.org/10.1523/JNEUROSCI.1541-18.2018

2018

Manipulating midbrain dopamine neurons and reward-related behaviors with light-controllable nicotinic acetylcholine receptors. Durand-de Cuttoli R, Mondoloni S, Marti F, Lemoine D, Nguyen C, Naudé J, d'Izarny-Gargas T, Pons S, Maskos U, Trauner D, Kramer RH, Faure P, Mourot A. eLife. DOI: 10.7554/eLife.37487

Design of a Highly Bistable Photoswitchable Tethered Ligand for Rapid and Sustained Manipulation of Neurotransmission. Lin WC, Tsai MC, Rajappa R, Kramer RH. Journal of the American Chemical Society. DOI: 10.1021/jacs.8b03942

BRestoring Vision to the Blind with Chemical Photoswitches. Tochitsky I, Kienzler MA, Isacoff E, Kramer RH. Chemical Reviews. DOI: 10.1021/acs.chemrev.7b00723

Restoration of patterned vision with an engineered photoactivatable G protein-coupled receptor. Berry MH, Holt A, Levitz J, Broichhagen J, Gaub BM, Visel M, Stanley C, Aghi K, Kim YJ, Cao K, Kramer RH, Trauner D, Flannery J, Isacoff EY. Nature Communications. DOI: 10.1038/s41467-018-03585-2

Light-Switchable Ion Channels and Receptors for Optogenetic Interrogation of Neuronal Signaling Lin WC, Kramer RH Bioconjugate Chemistry. DOI: 10.1021/acs.bioconjchem.7b00803

2017

Understanding and improving photo-control of ion channels in nociceptors with azobenzene photoswitches. Mourot A, Herold C, Kienzler MA, Kramer RH. British Journal of Pharmacology. DOI: 10.1111/bph.13923

Photopharmacological control of bipolar cells restores visual function in blind mice. Laprell L, Tochitsky I, Kaur K, Manookin MB, Stein M, Barber DM, Schön C, Michalakis S, Biel M, Kramer RH, Sumser MP, Trauner D, Van Gelder RN. The Journal of Clinical Investigation. DOI: 10.1172/JCI92156

Restoring visual function to the blind retina with a potent, safe and long-lasting photoswitch. Tochitsky I, Trautman J, Gallerani N, Malis JG, Kramer RH. Scientific Reports. DOI: 10.1038/srep45487

2016

How Azobenzene Photoswitches Restore Visual Responses to the Blind Retina. Tochitsky I, Helft Z, Meseguer V, Fletcher RB, Vessey KA, Telias M, Denlinger B, Malis J, Fletcher EL, Kramer RH. Neuron. DOI: 10.1016/j.neuron.2016.08.038

Serotonin modulates spike probability in the axon initial segment through HCN channels. Ko KW, Rasband MN, Meseguer V, Kramer RH, Golding NL. Nature Neuroscience. DOI: 10.1038/nn.4293

2015

Lateral Inhibition in the Vertebrate Retina: The Case of the Missing Neurotransmitter. Kramer RH, Davenport CM. PLOS Biology. DOI: 10.1371/journal.pbio.1002322

A Comprehensive Optogenetic Pharmacology Toolkit for In Vivo Control of GABAA Receptors and Synaptic Inhibition. Lin WC, Tsai MC, Davenport CM, Smith CM, Veit J, Wilson NM, Adesnik H, Kramer RH. Neuron. DOI: 10.1016/j.neuron.2015.10.026

Optopharmacological tools for restoring visual function in degenerative retinal diseases. Tochitsky I, Kramer RH. Current Opinion in Neurobiology. DOI: 10.1016/j.conb.2015.01.018

2014

Engineering a light-regulated GABAa receptor for optical control of neural inhibition. Lin WC, Davenport CM, Mourot A, Vytla D, Smith CM, Medeiros KA, Chambers JJ, Kramer RH. ACS Chemical Biology. DOI: 10.1021/cb500167u

Restoring visual function to blind mice with a photoswitch that exploits electrophysiological remodeling of retinal ganglion cells. Tochitsky I, Polosukhina A, Degtyar VE, Gallerani N, Smith CM, Friedman A, Van Gelder RN, Trauner D, Kaufer D, Kramer RH. Neuron. DOI: 10.1016/j.neuron.2014.01.003

Imaging an optogenetic pH sensor reveals that protons mediate lateral inhibition in the retina. Wang TM, Holzhausen LC, Kramer RH. Nature Neuroscience. DOI: 10.1038/nn.3627

2013

Optogenetic pharmacology for control of native neuronal signaling proteins. Kramer RH, Mourot A, Adesnik H. Nature Neuroscience. DOI: 10.1038/nn.3424

Light at the end of the channel: optical manipulation of intrinsic neuronal excitability with chemical photoswitches. Mourot A, Tochitsky I, Kramer RH. Frontiers in Molecular Neuroscience. DOI: 10.3389/fnmol.2013.00005

Photochromic potassium channel blockers: design and electrophysiological characterization. Mourot A, Fehrentz T, Kramer RH. Methods in Molecular Biology. DOI: 10.1007/978-1-62703-345-9_7

2012

Photochemical restoration of visual responses in blind mice. Polosukhina A, Litt J, Tochitsky I, Nemargut J, Sychev Y, De Kouchkovsky I, Huang T, Borges K, Trauner D, Van Gelder RN, Kramer RH. Neuron. DOI: 10.1016/j.neuron.2012.05.022

Optical control of endogenous proteins with a photoswitchable conditional subunit reveals a role for TREK1 in GABA(B) signaling. Sandoz G, Levitz J, Kramer RH, Isacoff EY. Neuron. DOI: 10.1016/j.neuron.2012.04.026

Rab3A mediates vesicle delivery at photoreceptor ribbon synapses. Tian M, Xu CS, Montpetit R, Kramer RH. The Journal of Neuroscience. DOI: 10.1523/JNEUROSCI.0265-12.2012

Rapid optical control of nociception with an ion-channel photoswitch. Mourot A, Fehrentz T, Le Feuvre Y, Smith CM, Herold C, Dalkara D, Nagy F, Trauner D, Kramer RH. Nature Methods. DOI: 10.1038/nmeth.1897

Optochemical control of genetically engineered neuronal nicotinic acetylcholine receptors. Tochitsky I, Banghart MR, Mourot A, Yao JZ, Gaub B, Kramer RH, Trauner D. Nature Chemistry. DOI: 10.1038/nchem.1234

Water-soluble, donor-acceptor biphenyl derivatives in the 2-(o-nitrophenyl)propyl series: highly efficient two-photon uncaging of the neurotransmitter γ-aminobutyric acid at λ = 800 nm. Donato L, Mourot A, Davenport CM, Herbivo C, Warther D, Léonard J, Bolze F, Nicoud JF, Kramer RH, Goeldner M, Specht A. Angewandte Chemie. DOI: 10.1002/anie.201106559

2011

Tuning photochromic ion channel blockers. Mourot A, Kienzler MA, Banghart MR, Fehrentz T, Huber FM, Stein M, Kramer RH, Trauner D. ACS Chemical Neuroscience. DOI: 10.1021/cn200037p

Release from the cone ribbon synapse under bright light conditions can be controlled by the opening of only a few Ca(2+) channels. Bartoletti TM, Jackman SL, Babai N, Mercer AJ, Kramer RH, Thoreson WB. Journal of Neurophysiology. DOI: 10.1152/jn.00634.2011

Engineering light-regulated ion channels. Fortin DL, Dunn TW, Kramer RH. Cold Spring Harbor Protocols. DOI: 10.1101/pdb.top112

LiGluR restores visual responses in rodent models of inherited blindness. Caporale N, Kolstad KD, Lee T, Tochitsky I, Dalkara D, Trauner D, Kramer R, Dan Y, Isacoff EY, Flannery JG. Molecular Therapy. DOI: 10.1038/mt.2011.103

A positive feedback synapse from retinal horizontal cells to cone photoreceptors. Jackman SL, Babai N, Chambers JJ, Thoreson WB, Kramer RH. Plos Biology. DOI: 10.1371/journal.pbio.1001057

Optogenetic photochemical control of designer K+ channels in mammalian neurons. Fortin DL, Dunn TW, Fedorchak A, Allen D, Montpetit R, Banghart MR, Trauner D, Adelman JP, Kramer RH. Journal of Neurophysiology. DOI: 10.1152/jn.00251.2011

2010 and earlier

Photochromic blockers of voltage-gated potassium channels. Banghart MR, Mourot A, Fortin DL, Yao JZ, Kramer RH, Trauner D. Angewandte Chemie. DOI: 10.1002/anie.200904504

New photochemical tools for controlling neuronal activity. Kramer RH, Fortin DL, Trauner D. Current Opinion in Neurobiology. DOI: 10.1016/j.conb.2009.09.004

Role of the synaptic ribbon in transmitting the cone light response. Jackman SL, Choi SY, Thoreson WB, Rabl K, Bartoletti TM, Kramer RH. Nature Neuroscience. DOI: 10.1038/nn.2267

Light-activated ion channels for remote control of neural activity. Chambers JJ, Kramer RH. Methods in Cell Biology. DOI: 10.1016/S0091-679X(08)00811-X

Photochemical control of endogenous ion channels and cellular excitability. Fortin DL, Banghart MR, Dunn TW, Borges K, Wagenaar DA, Gaudry Q, Karakossian MH, Otis TS, Kristan WB, Trauner D, Kramer RH. Nature Methods. 5: 331-8. DOI: 10.1038/nmeth.1187

Staples, tape measures, and bungee cords: a variety of bifunctional reagents for understanding and controlling ion channels. Mourot A, Kramer RH. ACS Chemical Biology. DOI: 10.1021/cb700135a

Remote control of neuronal activity with a light-gated glutamate receptor. Szobota S, Gorostiza P, Del Bene F, Wyart C, Fortin DL, Kolstad KD, Tulyathan O, Volgraf M, Numano R, Aaron HL, Scott EK, Kramer RH, Flannery J, Baier H, Trauner D, Isacoff EH. Neuron. DOI: 10.1016/j.neuron.2007.05.010

Synaptic Ca2+ in darkness is lower in rods than cones, causing slower tonic release of vesicles. Sheng Z, Choi SY, Dharia A, Li J, Sterling P, Kramer RH. The Journal of Neuroscience. DOI: 10.1523/JNEUROSCI.5386-06.2007

Light-induced depolarization of neurons using a modified Shaker K(+) channel and a molecular photoswitch. Chambers JJ, Banghart MR, Trauner D, Kramer RH. Journal of Neurophysiology. DOI: 10.1152/jn.00318.2006

Allosteric control of an ionotropic glutamate receptor with an optical switch. Volgraf M, Gorostiza P, Numano R, Kramer RH, Isacoff EY, Trauner D. Nature Chemical Biology. DOI: 10.1038/nchembio756

2005 and earlier

Photochemical tools for remote control of ion channels in excitable cells. Kramer RH, Chambers JJ, Trauner D. Nature Chemical Biology. DOI:10.1038/nchembio750L3

Encoding light intensity by the cone photoreceptor synapse. Choi SY, Borghuis BG, Borghuis B, Rea R, Levitan ES, Sterling P, Kramer RH. Neuron. DOI: 10.1016/j.neuron.2005.09.011

Imaging light-modulated release of synaptic vesicles in the intact retina: retinal physiology at the dawn of the post-electrode era. Choi SY, Sheng Z, Kramer RH. Vision Research. DOI: 10.1016/j.visres.2005.08.011

Light-activated ion channels for remote control of neuronal firing. Banghart M, Borges K, Isacoff E, Trauner D, Kramer RH. Nature Neuroscience. DOI: 10.1038/nn1356