Part 1: Plate 5 (50:50 hexane:acetone)

Repeat the spotting, developing, and visualization steps from plate 1 using a fifth plate, except this time, fill your developing chamber with 50:50 hexane : acetone.

Photo of TLC Plate 5, visualized with the naked eye. No spots are visible.

Visualization: Naked Eye

Photo of TLC Plate 5, visualized using a UV lamp. UV-active spots have been circled with a pencil. Spot C is very long and streaky, and spot As is somewhat long and streaky. Spots Ac and C are the most UV-active, and B and I are the least UV-active.

Visualization: UV Lamp

Solvent front: 4.9 cm. As: 2.9 cm, UV, no staining. Ac: 1.9 cm, UV, yellow. B: 4.7 cm, UV, no staining. C: 1.7 cm, UV, no staining. I: 4.0 cm, UV, no staining.

Visualization: Iodine

Copy down the stained plate, making sure to label the compound spotted in each lane of the plate, the solvent used to develop the plate, the solvent front, and how each spot was visualized. Then, calculate Rf values for each spot and add them to your data table.

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Next Page: Plate 6

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