Part 1: Plate 4 (80:20 hexane:acetone)

Repeat the spotting, developing, and visualization steps from plate 1 with a fourth plate, except this time, fill your developing chamber with 80:20 hexane : acetone.

Photo of TLC Plate 4, visualized with the naked eye. No spots are visible.

Visualization: Naked Eye

Photo of TLC Plate 4, visualized under a UV lamp. UV-active spots have been circled with a pencil. Ac and C are the most UV-active, while B and I are the least UV-active

Visualization: UV Lamp

Photo of plate 4, which has been stained with iodine. UV-active spots have been circled with a pencil. A ruler is located beside the plate. Solvent front: 4.4 cm. As: 0.7 cm, UV, pale yellow. Ac: 0.2 cm, UV, yellow. B: 3.3 cm, UV, no staining. C: 0.1 cm, UV, pale yellow. I: 1.2 cm, UV, no staining.

Visualization: Iodine

Copy down the stained plate, making sure to label the compound spotted in each lane of the plate, the solvent used to develop the plate, the solvent front, and how each spot was visualized. Then, calculate Rf values for each spot and add them to your data table.

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