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Aims
Aims
To carry out a practical to explore the techniques associated with paper chromatography.
To carry out a practical to explore the techniques associated with paper chromatography.
To separate and identify the amino acids present in an unknown sample.
To separate and identify the amino acids present in an unknown sample.
A. Report & Analyse Results
A. Report & Analyse Results
1. Picture your chromatogram showing separated amino acids.
2. Recorded the measurements for the solvent front and the distances moved by the amino acids.
3. Calculate the relative front values and then identify the amino acids in the solution.
This should all be evidenced in a table.
Things to consider CPAC 5a
Things to consider CPAC 5a
CPAC 5a - Uses appropriate software and/or tools to process data, carry out research and report findings.
1. Research and record the RF values of the listed amino acids from referenced & dated sources
2. Reports findings in own words with date and citation
Things to consider CPAC 5b
Things to consider CPAC 5b
CPAC 5b - Sources of information are cited demonstrating that research has taken place, supporting planning and conclusions.
· Carry out suitable research online from written sources about RF values and writes up in own words citing relevant information, facts and figures within the text as necessary.
. Use research to support conclusions drawn
· Clearly identify any material that is a direct quote as such.
· Compile a bibliography of texts from which material has been sourced in such a way that the reader may retrieve the information easily.
B. Evaluate your report by answering the questions
B. Evaluate your report by answering the questions
1. What amino acids did you identify as being present in the sample? (Use your research to back up your conclusions)
2. Why might not all of the amino acids known to be present in sample have appeared on your chromatogram?
3. Why are you advised to handle the chromatography paper as little as possible?
Math you will find useful
Math you will find useful
Rf stands for “relative front”.
It is the ratio of the distance moved by each amino acid to the distance moved by the solvent:
Rf = distance moved by spot
distance moved by solvent
Required Resources
Required Resources
Core Parctical 17 - Student Sheet.docxConsult CLEAPSS Hazcards® 66, 84B. Perform a risk assessment using up-to-date information before this practical is carried out
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