The annelids (Annelida, from Latin anellus, "little ring"), also known as the ringed worms or segmented worms, are a large phylum, with over 17,000 extant species including ragworms, earthworms, and leeches. The species exist in and have adapted to various ecologies – some in marine environments as distinct as tidal zones and hydrothermal vents, others in fresh water, and yet others in moist terrestrial environments.
The basic annelid form consists of multiple segments. Each segment has the same sets of organs and, in most polychaetes, has a pair of parapodia that many species use for locomotion.
Although many species can reproduce asexually and use similar mechanisms to regenerate after severe injuries, sexual reproduction is the normal method in species whose reproduction has been studied. The minority of living polychaetes whose reproduction and lifecycles are known produce trochophore larvae, that live as plankton and then sink and metamorphose into miniature adults. Oligochaetes are full hermaphrodites and produce a ring-like cocoon around their bodies, in which the eggs and hatchlings are nourished until they are ready to emerge.
Earthworms are Oligochaetes that support terrestrial food chains both as prey and in some regions are important in aeration and enriching of soil. The burrowing of marine polychaetes, which may constitute up to a third of all species in near-shore environments, encourages the development of ecosystems by enabling water and oxygen to penetrate the sea floor. In addition to improving soil fertility, annelids serve humans as food and as bait. Scientists observe annelids to monitor the quality of marine and fresh water. Although blood-letting is no longer in favor with doctors, some leech species are regarded as endangered species because they have been over-harvested for this purpose in the last few centuries. Ragworms' jaws are now being studied by engineers as they offer an exceptional combination of lightness and strength.
In this lab you will study the following groups of annelids:
Phylum Annelida
Class Errantia
Family Nereididae - Clam Worms
Class Sedentaria
Order Clitellata
Family Lumbricidae - Earthworms
Family Hirudinidae - Leeches
Below is a good overview of the Annelids and a look at some of their behaviors.
The Nereidids, also known as the bristle worms, are a family of annelid worms, generally marine. Each body segment has a pair of fleshy protrusions called parapodia that bear many bristles, called chaetae, which are made of chitin. As such, Nereidids are sometimes referred to as bristle worms. More than 10,000 species are described in this family. Common representatives include the lugworm (Arenicola marina) and the sandworm or clam worm, Nereis.
Nereidids as a family are robust and widespread, with species that live in the coldest ocean temperatures of the abyssal plain, to forms which tolerate the extremely high temperatures near hydrothermal vents. Nereidids occur throughout the Earth's oceans at all depths, from forms that live as plankton near the surface, to a 2- to 3-cm specimen (still unclassified) observed by the robot ocean probe Nereus at the bottom of the Challenger Deep, the deepest known spot in the Earth's oceans. Only 168 species (less than 2% of all Nereidids) are known from fresh waters.
This clam worm (genus Nereis) looks superficially like a centipede but the extensions along the side of the body are not legs but are structures called parapodia that aid in movement and in respiration.
Instructional video on clam worm (Nereis) movement
Note: Focus on how movement is produced and what structures are involved.
Watch the video showing movement of Nereis.
Observe how the body moves in a side-to-side (sinusoidal) pattern.
Identify the role of parapodia in locomotion, noting how they act like paddles or limbs.
Compare this movement to that of an earthworm.
Recognize that earthworms move using peristalsis (waves of contraction and expansion).
Determine how the use of parapodia and lateral movement in Nereis differs from the forward, segment-by-segment motion of earthworms.
Be prepared to answer the Knowledge Check questions.
No materials to return. Ensure the video is closed when finished.
Whole specimen of Nereis (clam worm)
Dissecting microscope
Note: Handle specimens carefully. Use low magnification first.
Place the specimen under the dissecting microscope and observe overall body structure.
Identify the lateral appendages called parapodia.
Locate the bristle-like setae extending from the parapodia.
Examine the anterior region and identify the peristomium.
Observe the jaw structures within the anterior region (may require adjustment of focus or gentle manipulation).
Locate the cirri extending from the parapodia or head region.
Identify the anus at the posterior end.
Return the specimen to the designated container.
Clean all materials and work surfaces.
Lumbricidae is a family of animals in the phylum Annelida, which is made up of many types of aquatic and terrestrial worms, and including all of the various earthworms. Specifically, the lumbricids contain the terrestrial megadrile earthworms (some of which are semiaquatic or fully aquatic), and freshwater or semiterrestrial microdrile forms, including the tubificids, pot worms and ice worms (Enchytraeidae), blackworms and several interstitial marine worms.
With around 10,000 known species, the Lumbricids make up about half of the phylum Annelida. These worms usually have few setae (chaetae) or "bristles" on their outer body surfaces, and lack parapodia, unlike polychaet worms (Class Errantia; Family Nereididae).
Lumbricids are well-segmented worms and most have a spacious body cavity (coelom) used as a hydroskeleton. They range in length from less than 0.5 mm (0.02 in) up to 2 to 3 metres (6.6 to 9.8 ft) in the 'giant' species such as the giant Gippsland earthworm (Megascolides australis).
Terrestrial oligochaetes are commonly known as earthworms and burrow into the soil. Earthworms are found in all parts of the world except for deserts. They have a requirement for moist surroundings and the larger species create burrows that may go down several meters (yards) while young individuals and smaller species are restricted to the top few centimeters of soil. The largest numbers are found in humus-rich soils and acid soils. A few species are found in trees, among damp moss and in the debris that accumulates in leaf axils and crevices; some others make their homes in the rosettes of bromeliads.
The majority of aquatic oligochaetes are small, slender worms, whose organs can be seen through the transparent body wall. They burrow into the sediment or live among the vegetation mostly in shallow, freshwater environments.
Earthworms are common soil organisms and are important in aerating soil and providing nutrients.
Instructional video on earthworm movement
Note: Focus on how movement is produced and what structures are involved.
Watch the video showing earthworm movement.
Observe how the body moves using peristalsis (waves of contraction and expansion).
Identify how the circular and longitudinal muscles contribute to movement.
Compare this movement to that of clam worms (Nereis).
Recognize that earthworms lack parapodia and move without lateral paddling structures.
Determine how earthworm locomotion differs from the side-to-side, appendage-assisted movement seen in clam worms.
Be prepared to answer the Knowledge Check questions.
No materials to return. Ensure the video is closed when finished.
Earthworm specimen
Dissecting tray
Dissecting microscope or hand lens
Note: Handle specimens carefully and keep them moist during observation.
Place the earthworm in the dissecting tray and observe overall body organization.
Identify the repeating segments along the body.
Locate the clitellum, the thickened band on the anterior portion.
Identify the prostomium at the anterior end.
Locate the mouth just posterior to the prostomium.
Observe the small bristles (setae) along the body segments.
Identify the anus at the posterior end.
Return the specimen as instructed by your instructor.
Clean all materials and work surfaces.
Earthworm specimen (live or preserved)
Dissecting tray and tools
Instructional dissection video
Note: Follow instructor guidelines for euthanasia if using a live specimen. Handle dissection tools carefully.
Place the earthworm in the dissecting tray and position it dorsal side up.
Follow the dissection video, making a shallow longitudinal incision along the dorsal surface.
Open the body cavity carefully and secure the body wall as needed.
Identify the internal partitions (septa) between segments.
Locate the pharynx at the anterior end, followed by the esophagus.
Identify the crop and the muscular gizzard.
Trace the intestine running posteriorly through the body.
Locate the seminal vesicles associated with reproduction.
Identify the aortic arches (hearts) surrounding the esophagus.
Dispose of specimens as instructed.
Clean and return all dissection tools.
Wipe down work surfaces and wash hands.
Leeches are segmented worms that belong to the phylum Annelida and comprise the family Hirudinidae. Like the lumbricids, such as earthworms, leeches share a clitellum and are hermaphrodites. Nevertheless, they differ from the lumbricids in significant ways. For example, leeches do not have bristles and the external segmentation of their bodies does not correspond with the internal segmentation of their organs. Their bodies are much more solid as the spaces in their coelom are dense with connective tissues. They also have two suckers, one at each end.
The majority of leeches live in freshwater environments, while some species can be found in terrestrial and marine environments. The best-known leeches, such as the medicinal leech, Hirudo medicinalis, are hematophagous, feeding on vertebrate blood and invertebrate hemolymph. Most leech species, however, are predatory, feeding primarily by swallowing other invertebrates.
Almost 700 species of leeches are currently recognized, of which some 100 are marine, 90 terrestrial and the remainder freshwater taxa.
Leeches, such as the Hirudo medicinalis, have been historically used in medicine to remove blood from patients. The practice of leeching can be traced to ancient India and Greece, and continued well into the 18th and 19th centuries in both Europe and North America. In modern times, leeches are used medically in procedures such as the reattachment of body parts and reconstructive and plastic surgeries and, in Germany, treating osteoarthritis.
This is an image of a typical leech. While many of them are ectoparasites quite a few species are also free living and are not parasites.
Instructional video(s) on leech movement
Note: Focus on how movement is produced and what structures are involved.
Watch the video(s) showing leech movement.
Observe how leeches use anterior and posterior suckers for movement.
Identify the looping (inchworm-like) motion used during crawling.
Observe any swimming behavior and note differences from crawling.
Compare leech movement to that of earthworms.
Determine how the use of suckers differs from movement by peristalsis.
Be prepared to answer the Knowledge Check questions.
No materials to return. Ensure the video is closed when finished.
Preserved leech specimen
Dissecting microscope or hand lens
Note: Handle specimens carefully and use low magnification first.
Place the specimen under the dissecting microscope and observe overall body shape.
Identify the anterior sucker at the front end.
Locate the mouth within the anterior sucker.
Identify the posterior sucker at the rear end.
Observe the body and identify the external segments.
Return the specimen to the designated container.
Clean all materials and work surfaces.
Watch the video below to discover how modern day medicine still uses leeches to help some patients increase blood circulation.