Institute of Arctic Biology's Genomics Core Laboratory
Instruments
Instruments
NOTE: Before using an instrument for the first time, please see the Core Lab coordinator for training (ljmullen2@alaska.edu; x7336).
NOTE: Before using an instrument for the first time, please see the Core Lab coordinator for training (ljmullen2@alaska.edu; x7336).
Most Popular
Most Popular
Illumina Miseq
Illumina Miseq
The industry leading instrument for focused applications such as targeted gene sequencing, amplicon sequencing, metagenomics, small genome sequencing, targeted gene expression, HLA typing, QC for HiSeq libraries, and more.
The industry leading instrument for focused applications such as targeted gene sequencing, amplicon sequencing, metagenomics, small genome sequencing, targeted gene expression, HLA typing, QC for HiSeq libraries, and more.
MiSeq reagents enable up to 15 Gb of output with 25 M sequencing reads and 2x300 bp read lengths.
MiSeq reagents enable up to 15 Gb of output with 25 M sequencing reads and 2x300 bp read lengths.
Please see our amplicon sequencing pricing summary here. Please contact the Genomics Core Lab coordinator for a quote. Sample submission guidelines can be found here.
Please see our amplicon sequencing pricing summary here. Please contact the Genomics Core Lab coordinator for a quote. Sample submission guidelines can be found here.
Data Retention Policy: All data generated will be made immediately available to researchers via the BaseSpace sequence hub . It is important that researchers make copies of their data and immediately back it up. It is recommended that at least two copies be made and stored in separate locations. The Genomics Core Lab will only guarantee data storage for 90 days.
Data Retention Policy: All data generated will be made immediately available to researchers via the BaseSpace sequence hub . It is important that researchers make copies of their data and immediately back it up. It is recommended that at least two copies be made and stored in separate locations. The Genomics Core Lab will only guarantee data storage for 90 days.
The Illumina MiSeq may only be operated by the trained technician.
The Illumina MiSeq may only be operated by the trained technician.
NanoDrop (One and ND-1000)
NanoDrop (One and ND-1000)
Microvolume spectrophotometer using 1-2µl of sample. Used for:
Microvolume spectrophotometer using 1-2µl of sample. Used for:
Nucleic acid concentration and quality assessment
Nucleic acid concentration and quality assessment
Purified protein analysis
Purified protein analysis
Cell density measurements
Cell density measurements
Bradford Assay analysis of protein
Bradford Assay analysis of protein
general UV-vis spectrophotometry
general UV-vis spectrophotometry
Qubit 2.0
Qubit 2.0
Benchtop fluorometer for the quantitation of DNA, RNA, protein and other molecules. All reagents and consumables are user supplied.
Benchtop fluorometer for the quantitation of DNA, RNA, protein and other molecules. All reagents and consumables are user supplied.
Kingfisher Apex
Kingfisher Apex
Automated high-throughput DNA, RNA, and protein purification instrument. 96 Deep Well and 24 Combi heads and heating blocks installed.
Automated high-throughput DNA, RNA, and protein purification instrument. 96 Deep Well and 24 Combi heads and heating blocks installed.
Imaging Systems
Imaging Systems
Amersham Imager (AI680UV)
Amersham Imager (AI680UV)
High sensitivity imager for UV, chemiluminescence and epi-white light imaging. No Ethidium Bromide or SYBR stains.
High sensitivity imager for UV, chemiluminescence and epi-white light imaging. No Ethidium Bromide or SYBR stains.
ChemiDocIt2
ChemiDocIt2
Used for visualization of most blots, gels and colony counting applications. No Ethidium Bromide or SYBR stains.
Used for visualization of most blots, gels and colony counting applications. No Ethidium Bromide or SYBR stains.
Amersham Storm 860
Amersham Storm 860
The Storm system is an optical scanner that produces digital images of radioactive or fluorescently labeled samples.
The Storm system is an optical scanner that produces digital images of radioactive or fluorescently labeled samples.
Accessories:
Accessories:
Image Eraser-Erases storage phosphor screens for reuse
Image Eraser-Erases storage phosphor screens for reuse
Storage phosphor screen-General-purpose storage phosphor screen that is 20cm x 25 cm
Storage phosphor screen-General-purpose storage phosphor screen that is 20cm x 25 cm
Exposure cassette- Two small cassettes for unmounted screens
Exposure cassette- Two small cassettes for unmounted screens
Equipment
Equipment
Centrifuges
Centrifuges
We have a diverse array of Pre- and post-PCR centrifuges that can accommodate PCR tubes, strips and plates, microcentrifuge tubes, 15 and 50ml Falcon tubes and more. For specific needs, contact Logan Mullen.
We have a diverse array of Pre- and post-PCR centrifuges that can accommodate PCR tubes, strips and plates, microcentrifuge tubes, 15 and 50ml Falcon tubes and more. For specific needs, contact Logan Mullen.
Of particular note:
Of particular note:
Beckman Coulter L-100XP Optima Ultracentrifuge
Beckman Coulter L-100XP Optima Ultracentrifuge
Sorvall RC 5C Plus
Sorvall RC 5C Plus
Pre- and post-PCR vacufuges
Pre- and post-PCR vacufuges
PCR Hoods
PCR Hoods
Two PCR hoods equipped with UV light sterilization are available for use with all pre-PCR needs. Training is required before use.
Two PCR hoods equipped with UV light sterilization are available for use with all pre-PCR needs. Training is required before use.
Thermal Cyclers
Thermal Cyclers
Quantitative and Digital PCR instruments must be reserved. Training is required before use. General PCR thermal cyclers on a first come, first serve basis.
Quantitative and Digital PCR instruments must be reserved. Training is required before use. General PCR thermal cyclers on a first come, first serve basis.
QuantStudio Absolute Q dPCR
QuantStudio Absolute Q dPCR
MAP 16 plates (16 samples) can be run in 1 column (4 sample) increments
MAP 16 plates (16 samples) can be run in 1 column (4 sample) increments
Four available channels (FAM, VIC/HEX, ABY, and JUN/Cy5) plus 5th dedicated ROX normalization channel
Four available channels (FAM, VIC/HEX, ABY, and JUN/Cy5) plus 5th dedicated ROX normalization channel
Analytik-Jena qTOWER3 84 G qPCR
Analytik-Jena qTOWER3 84 G qPCR
384 well, temperature gradient capable
384 well, temperature gradient capable
Filters installed for use with FAM, Sybr Green, Alexa488, ROX, TexasRed, and Cy3.5, among others. Additional filters can be purchased from manufacturer.
Filters installed for use with FAM, Sybr Green, Alexa488, ROX, TexasRed, and Cy3.5, among others. Additional filters can be purchased from manufacturer.
ABI 7900HT qPCR
ABI 7900HT qPCR
384 well and 96 well compatible
384 well and 96 well compatible
General PCR
General PCR
ABI Veriti 96 well PCR (3 for PCR, 1 for pre-PCR incubations)
ABI Veriti 96 well PCR (3 for PCR, 1 for pre-PCR incubations)
ABI Veriti 96 well Fast PCR
ABI Veriti 96 well Fast PCR
ABI Veriti 384 well PCR
ABI Veriti 384 well PCR
ABI ProFlex PCR
ABI ProFlex PCR
Blue Pippin
Blue Pippin
Automated size selection using gel cassettes. An important part of NGS library preparation. Varying gel cassettes can accommodate fragment selection of 90bp-50kb.
Automated size selection using gel cassettes. An important part of NGS library preparation. Varying gel cassettes can accommodate fragment selection of 90bp-50kb.
Consumables are typically user supplied but certain NGS applications may be carried out by the Core Lab for an additional fee.
Consumables are typically user supplied but certain NGS applications may be carried out by the Core Lab for an additional fee.
Agilent 2100 Bioanalyzer
Agilent 2100 Bioanalyzer
Routinely used by the Genomics Core Lab for Illumina library preparation quality control. Determines RNA, DNA and protein concentration and size in 12 samples.
Routinely used by the Genomics Core Lab for Illumina library preparation quality control. Determines RNA, DNA and protein concentration and size in 12 samples.
Agilent TapeStation 4200
Agilent TapeStation 4200
The Agilent 4200 TapeStation system is an established automated electrophoresis tool for DNA and RNA sample quality control. Fully automated sample processing enables the unattended analysis of size, concentration and integrity. Choose any sample number between 1 and 96 and analyze at constant cost per sample.
The Agilent 4200 TapeStation system is an established automated electrophoresis tool for DNA and RNA sample quality control. Fully automated sample processing enables the unattended analysis of size, concentration and integrity. Choose any sample number between 1 and 96 and analyze at constant cost per sample.
Lyophilizer
Lyophilizer
Virtis condenser unit (-40 C) with upgraded scroll pump. Can accommodate samples in external flasks or on internal racks.
Virtis condenser unit (-40 C) with upgraded scroll pump. Can accommodate samples in external flasks or on internal racks.
BioTek-Synergy H1MG
BioTek-Synergy H1MG
Synergy™ H1 is a configurable multi-mode microplate reader. Choose monochromator-based optics for flexibility, filter-based optics for sensitivity, or both…BioTek’s patented Hybrid Technology™ offers high-performance and applications versatility in a modular platform to expand as your laboratory’s needs change. Top and bottom fluorescence intensity, UV-visible absorbance and luminescence detection are available to meet many life science research requirements. Capable of CO2/O2 incubation assays.
Synergy™ H1 is a configurable multi-mode microplate reader. Choose monochromator-based optics for flexibility, filter-based optics for sensitivity, or both…BioTek’s patented Hybrid Technology™ offers high-performance and applications versatility in a modular platform to expand as your laboratory’s needs change. Top and bottom fluorescence intensity, UV-visible absorbance and luminescence detection are available to meet many life science research requirements. Capable of CO2/O2 incubation assays.
BioTek-Epoch
BioTek-Epoch
A standard UV-vis 96 well plate reader for spectrophotometric measurements (200-999nm). An associated plate washer is available for use.
A standard UV-vis 96 well plate reader for spectrophotometric measurements (200-999nm). An associated plate washer is available for use.
Spectramax Gemini EM
Spectramax Gemini EM
Create custom fluorescence assays without having to purchase expensive filter sets. The top- and bottom-reading Gemini EM Microplate Reader with dual monochromators lets users determine the optimal excitation and emission settings for creative new fluorescence intensity assays. If the best wavelengths are unknown, the Gemini EM reader will find them. Switch easily between top and bottom reading modes for solution- and cell-based assays.
Create custom fluorescence assays without having to purchase expensive filter sets. The top- and bottom-reading Gemini EM Microplate Reader with dual monochromators lets users determine the optimal excitation and emission settings for creative new fluorescence intensity assays. If the best wavelengths are unknown, the Gemini EM reader will find them. Switch easily between top and bottom reading modes for solution- and cell-based assays.
Millipore Muse Cell Analyzer
Millipore Muse Cell Analyzer
Compact microcapillary flow cytometer for quantitative analsis of suspension and adherent cells 2-60 μm in diameter. This instrument can evaluate up to 3 cellular parameters – cell size (forward scatter) and 2 colors (detected in the red and/or yellow channels). All-in-one kits are available for analyzing cell count and viability, apoptosis, autophagy, oxidative stress, and cell signaling.
Compact microcapillary flow cytometer for quantitative analsis of suspension and adherent cells 2-60 μm in diameter. This instrument can evaluate up to 3 cellular parameters – cell size (forward scatter) and 2 colors (detected in the red and/or yellow channels). All-in-one kits are available for analyzing cell count and viability, apoptosis, autophagy, oxidative stress, and cell signaling.
Milli Q Water
Milli Q Water
The Milli-Q EQ 7000 water purification system produces pyrogen and nuclease free water with a TOC (Total Organic Carbon) content of ≤5 ppb. The flow rate is adjustable up to a maximum of 2 L/min. A specific volume of water can be dispensed via the "Volumetric" option on the touchscreen interface and the speed of dispensed water can be changed by twisting the blue button on top of the dispenser. Water will automatically stop dispensing after 15 minutes (roughly 30 L).
The Milli-Q EQ 7000 water purification system produces pyrogen and nuclease free water with a TOC (Total Organic Carbon) content of ≤5 ppb. The flow rate is adjustable up to a maximum of 2 L/min. A specific volume of water can be dispensed via the "Volumetric" option on the touchscreen interface and the speed of dispensed water can be changed by twisting the blue button on top of the dispenser. Water will automatically stop dispensing after 15 minutes (roughly 30 L).
Illumina Bead Station
Illumina Bead Station
The Illumina BeadStation 500X supports Gene Expression profiling of focused sets of genes and whole genomes. It enables a wide range of sample throughput (16-192 samples/day) and broad experimental scale (6000-300,000 genotypes per day). The Bead Array Reader is a confocal scanner with < 1 micron resolution for use with Sentrix® Array Matrices and Sentrix® BeadChips. The Core Lab also has the Illumina Hybridization oven with BeadChip Hyb wheel model 5420.
The Illumina BeadStation 500X supports Gene Expression profiling of focused sets of genes and whole genomes. It enables a wide range of sample throughput (16-192 samples/day) and broad experimental scale (6000-300,000 genotypes per day). The Bead Array Reader is a confocal scanner with < 1 micron resolution for use with Sentrix® Array Matrices and Sentrix® BeadChips. The Core Lab also has the Illumina Hybridization oven with BeadChip Hyb wheel model 5420.
Genomics Core Lab Coordinator
Genomics Core Lab Coordinator
Logan Mullen
216 West Ridge Research Building
Phone: 907-474-7336
ljmullen2@alaska.edu