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New: Project-based learning module
From leaf to lineage: SSR-marker based genotyping and analysis
From leaf to lineage: SSR-marker based genotyping and analysis
Short Description: Three-day module gives undergraduate students a project-based learning experience in molecular genetics using Simple Sequence Repeat (SSR) marker-based genotyping for wheat diversity analysis.
Type: Hands-on lab with focus on scientific writing, literature survey and using reference management tools.
Duration: Three days 09:00 AM- 06:00 PM (27 hours)
Day 1: 05-Dec-2025 (Friday)
Day 2: 06-Dec-2025 (Saturday)
Day 3: 07-Sep-2025 (Sunday)
No of participants: Only Six
Aimed at: Students currently enrolled in F.Y, S.Y or T.Y. B.Sc (Life Sciences)
Masters students or 4th year B.Sc. (Hons) students should NOT apply, if you do then fees will not be refunded.
Fees: INR 6490 (INR 5500 + 18% GST)
Category (for payment): MBY Workshop Tier 5
Application Process: Click here for the details
Salient features
Salient features
Only one scaffolded project-based learning module spread over 03 full days
Conducted with 06 participants and two full-time, highly qualified instructors.
Participants get to set-up their own PCR reactions and AGE.
Process of writing a scientific article explained
Use of modern tools for literature survey and reference management.
Participants to submit individual reports based on their collective experimental results.
Dedicated troubleshooting session to develop critical thinking about experimental errors and data quality.
Application process (in brief)
Application process (in brief)
Dates are fixed. 05-06-07 December-2025.
Note down the category of the workshop.
Head to SB Collect to pay the fees. Save the Receipt and the SB Collect Reference number. See here for details.
Fill the above Registration form using the Receipt and the SB Collect Reference number. Choose the workshop date. You will get an email acknowledgement from us. Registration is NOT yet complete.
We verify your payment and send Registration Confirmation Email. Your registration is now complete.
We strongly encourage the applicants to look at the detailed application process before paying the fees and filling the form.
Course Content
Course Content
1. Basic laboratory practices
1. Basic laboratory practices
Basic safety precautions, handling of micropipettes and safe disposal of laboratory wastes. To know more about how to handle micropipettes, watch this video or or practice using this simulation.
2. Extraction of plant genomic DNA
2. Extraction of plant genomic DNA
The first step in order to amplify a specific gene from the whole genome is to extract the genomic DNA from leaf tissue of six different wheat varieties. This is done using a kit. To know more about kit-based DNA extraction, watch this video.
3. Quantitative measurement of DNA
3. Quantitative measurement of DNA
Exact amount of DNA extracted from wheat leaves is measured using a test based on spectrophotometry. To know more about spectrophotometry, you can read this article or watch this video for how a spectrophotometer works.
4. Gradient Polymerase Chain Reaction (PCR)
4. Gradient Polymerase Chain Reaction (PCR)
The simplest way to identify SSR-based markers from wheat genome is using Polymerase Chain Reaction (PCR). Gradient PCR is done to detect and screen polymorphism in multiple SSR genes. To know more about PCR, you can either read this article or watch this video.
5. Agarose Gel Electrophoresis (AGE)
5. Agarose Gel Electrophoresis (AGE)
The extracted genomic DNA are checked using AGE to confirm whether the extraction has happened properly or not. Also gradient PCR results are scored using the AGE results. To know more about gel electrophoresis, you can either read this article or watch this video.
6. Data Scoring and phylogenetic tree construction
6. Data Scoring and phylogenetic tree construction
Scoring of the PCR results obtained by the participants. Using online software (demonstration and practical session) to construct phylogenetic trees and interpreting genetic diversity among the different wheat varieties.
7. In silico primer designing
7. In silico primer designing
Students use their laptops and an online tool to design SSR primers.
8. Scientific Communication & Research Literacy
8. Scientific Communication & Research Literacy
Part of the PBL is dedicated to crucial scientific writing skills to ensure that the project culminates in a high-quality report.
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