New workshop: LAMP
Short Description: Use molecular biology tools in cotton plant (kit-based extraction of DNA, PCR, AGE and LAMP) to detect the genetically modified (GMO) cotton variety.
Type: Hands-on
Duration: One day
Fees: INR 2596 (INR 2200 + 18% GST)
Category (for payment): MBY Workshop Tier 2
Aimed at: College students
Application Process: Click here for the details
Check the above table to figure out whether suitable dates are available or not.
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Fill the above Registration form using the Receipt and the SB Collect Reference number. Choose the workshop date. You will get an email acknowledgement from us. Registration is NOT yet complete.
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We strongly encourage the applicants to look at the detailed application process before paying the fees and filling the form.
Basic safety precautions, handling of micropipettes and safe disposal of laboratory wastes. To know more about how to handle micropipettes, watch this video or or practice using this simulation.
The first step in order to amplify a specific gene from the whole genome is to extract the genomic DNA from cotton leaf tissue. This is done using a kit. To know more about kit-based DNA extraction, watch this video.
A single-tube technique for the amplification of DNA for diagnostic and GMO detection purposes which does not require a thermal cycler. Instead of a series of alternating temperature cycles, this amplification is carried out at a constant temperature. To know more about LAMP, you can read this article on how LAMP works.
The simplest way to detect if an organism is genetically modified is using Polymerase Chain Reaction (PCR). PCR amplifies the number of copies of any gene of our interest (here we detect the recombinant gene). To know more about PCR, you can either read this article or watch this video.
The extracted DNA and PCR products are checked using AGE to confirm whether the extraction and amplification has happened properly or not. To know more about gel electrophoresis, you can either read this article or watch this video.