The FLIC system was co-developed by former Graduate Students Jenny Ro and Zachary Harvanek, along with Dr. Scott Pletcher, for the purpose of accurately measuring & recording the liquid food intake of individual flies over a period of time and under controlled conditions. The system is currently in use in several Drosophila laboratories across the United States, the United Kingdom,and China. For further information, please see WikiFlic.
The FLIC setup
Capturing neural activity in freely moving flies using CaMPARI captured by a high-powered Ultra-Violet LED through three aspheric condenser lenses.
FLIC Real-time demonstration; Dr. Scott Pletcher narrating.
Drosophila have a natural tendency to climb. The D-Drop takes advantage of this inherent behavior to determine overall fitness of a small group of flies, by forcing them to the bottom of a narrow channel using the force of gravity and acceleration. followed by a sudden stop. Tracking software, developed in-house, then tracks the fly movement as they naturally climb back up to the top of the channel. As the flies age over days and weeks, they presumably react slower as they climb back up. This device offers an accelerated method of gauging the flies health, as it is tied to their climbing abilities over time.
The D-Drop Deluxe.
(Drosophila Activity Monitor)
Lid with LED's for the horizontal DAM unit (inverted).
OPTO-Lifespan
12 Vial rig, using blue LED's.
Note: in lower picture, monitor wall paper is a random internet photo.
Publications that include the FLIC in use can be found here:
Sergio Hidalgo, Joanna C Chiu. 06 Apr 2023, 12:374, CRUMB: a shiny-based app to analyze rhythmic feeding in Drosophila using the FLIC system (https://doi.org/10.12688/f1000research.132587.1)
Yu-Chieh David Chen, Vaibhav Menon, Ryan Matthew Joseph and Anupama Arun Dahanukar. Journal of Neuroscience 7 July 2021, 41 (27) 5791-5808; Control of Sugar and Amino Acid Feeding via Pharyngeal Taste Neurons DOI: https://doi.org/10.1523/JNEUROSCI.1794-20.2021
May CE, Rosander J, Gottfried J, Dennis E, Dus M. Jun 16, 2020. Dietary sugar inhibits satiation by decreasing the central processing of sweet taste
May CE1, Vaziri A2, QiLin Y3, , Grushko O4 ,Khabiri M5 ,Wang Q-P36 ,Holme KJ7 , Pletcher SD7, Freddolino PL58, Neely GG3 ,Dus M1249 May 2019. High Dietary Sugar Reshapes Sweet Taste to Promote Feeding Behavior in Drosophila melanogaster. 3
Shell, B.C., Schmitt, R.E., Lee, K.M. et al. Measurement of solid food intake in Drosophila via consumption-excretion of a dye tracer. Sci Rep 8, 11536 (2018). https://doi.org/10.1038/s41598-018-29813-9
Ro J, et al 2016. Serotonin signaling mediates protein valuation and aging
Qi Zhang Dissertation. 2016. Characterization of Circadian Feeding Rhythms in Drosophila Using the Fly Liquid-Food Interaction Counter (FLIC) Assay
Vivien Marx. 2015, July VOL.12 NO.7 Metabolism: feeding fruit flies DOI https://doi.org/10.1038/nmeth.3443.
Linford N, Ro J, Chung B, Pletcher S. 2015. Gustatory and metabolic perception of nutrient stress in Drosophila