Publications
Publications (2021-present)
130. Z. Cheng, M. Aitha, C. Thomas, A. Sturgill, M. Fairweather, A. Hu, C. Bethel, D. Rivera, P.
Dranchak, P. Thomas, H. Li, Q. Feng, K. Tao, M. Song, N. Sun, S. Wang, S. Silwal, R. Page, W. Fast, R. Bonomo, M. Weese, W. Martinez, J. Inglese, M.W. Crowder, "Machine Learning Models Identify Inhibitors of New Delhi Metallo-β-lactamase (NDM)", J. Chem. Inform. Modeling (2024) accepted for publication.
129. Rui Xu, Huan Zhang, Michael W Crowder, Jiangjiang Zhu, Multiple and Optimal Screening Subset: a method selecting global characteristic congeners for robust foodomics analysis, Briefings in Bioinformatics (2024) 25 (2) bbae046, https://doi.org/10.1093/bib/bbae046
128. Benin, B.M.; Hillyer, T.; Crugnale, A.S.; Fulk, A.; Thomas, C.A.; Crowder, M.W.; Smith, M.A.; Shin, W.S. Taxifolin as a Metallo-β-Lactamase Inhibitor in Combination with Augmentin against Verona imipenemase 2 Expressing Pseudomonas aeruginosa. Microorganisms (2023) 11, x. https://doi.org/10.3390/xxxxx.
127. C. Thomas, Z. Cheng, C. Bethel, A. Hujer, A. Sturgill, K. Onuoha, R. Page, R. Bonomo, M. Crowder, "The Directed Evolution of NDM-1" Antimicro. Agents Chemo. (2023) accepted for publication.
126. R. Xu, L. Chen, H. Zhang, M.W. Crowder, J. Zhu, “Characterizing bourbon whiskey via the combination of LC-MS and GC-MS based molecular fingerprinting” (2023) Food Chem. 423 136311.
125. H. Zhang, E.C. Johnson, A. Carroll, C. Pryor, K. Howe, R. Kramer, R. Naik, E. Meyer, B. Shurina, N. Kumarage, K. Yang, M.A. Kennedy, M.W. Crowder, “Profiling bourbons based on congener concentrations” J. Food Comp. Analys. (2023) 115 105019.
124. A. Legru, F. Verdirosa, Y. Vo-Hoang, G. Tassone, F. Vascon, C.A. Thomas, F. Sannio, G. Corsica, M. Benvenuti, G. Feller, R. Coulon, F. Marcoccia, S.R. Devente, E. Bouajila, C. Piveteau, F. Leroux, R. Deprez-Poulain, B. Deprez, P. Licznar-Fajardo, M.W. Crowder, L. Cendron, C. Pozzi, S. Mangani, J.-D. Docquier, J.-F. Hernandez, L. Gavara; “Optimization of 1,2,4-Triazole-3-thiones toward Broad-Spectrum Metallo-β-lactamase Inhibitors Showing Potent Synergistic Activity on VIM- and NDM-1-Producing Clinical Isolates” J. Med. Chem. (2022) 65 (24) 16392-16419.
123. P.W. Thomas, E.J. Cho, C.R. Bethel, T. Smisek, Y.-C. Ahn, J.M. Schroeder, C.A. Thomas, K.N. Dalby, J.T. Beckham, M.W. Crowder, R.A. Bonomo, W. Fast, "Discovery of an effective small-molecule allosteric inhibitor of New Delhi metallo-b-lactamase (NDM) " ACS Infect. Dis. (2022) 8 (4) 811-824.
122. H. Yu, E. Brewer, M. Shields, M. Crowder, C. Sacchetti, B. Soontornniyomkij, D. Dou, B. Clemente, M. Sablad, P. Chivukula, S. Hughes, S. Roberts, K. Rajappan, S. Tannis, R. Sekulovich, S. Parker, P. Limphong, “Restoring ornithine transcarbamylase (OTC) activity in OTC-deficient mouse model using LUNAR-OTC mRNA” Clin. Translat. Dis. (2022) 2 e33. (impact factor: 4.7)
121. A. Legru, F. Verdirosa, J.-F. Hernandez, G. Tassone, F. Sannio, M. Benvenuti, P.-A. Conde, G Bossis, C.A. Thomas, M.W. Crowder, M. Dillenberger, K. Becker, C. Pozzi, S. Mangani, J.-D. Docquier, and L. Gavara, “1,2,4-Triazole-3-thione compounds with a 4-ethyl alkyl/aryl sulfide substituent are broad-spectrum metallo-β-lactamase inhibitors with re-sensitization activity” Eur. J. Med. Chem. (2021) 226 113873.
120. W.S. Shin, M.E. Nguyen, A. Bergstrom, I.R. Jennings, M.W. Crowder, R. Muthyala, Y.Y. Shin, "Fragment-based screening and hit-based substructure search: Rapid discovery of 8-hydroxyquinonline-7-carboxylic acid as a low-cytotoxic, broad spectrum nanomolar metallo-b-lactamase inhibitor" Chem Biol Drug Design (2021) 98 481-492.
119. R. Mehta, D. Rivera, D. Reilley, D. Tan, P. Thomas, A. Hinojosa, A. Stewart, Z. Cheng, C. Thomas, M. Crowder, A. Anastassia, W. Fast, and E. Que, "Visualizing the dynamic metallation state of NDM-1 in bacteria using a reversible fluorescent probe" J. Am. Chem. Soc. (2021) 143 (22) 8314-8323.
118. H. Zhang, K. Yang, Z. Cheng, C. Thomas, A. Steinbrunner, C. Pryor, M. Vulcan, C. Kemp, D. Orea, C. Paththamperuma, A.Y. Chen, S.M. Cohen, R.C. Page, D.L. Tierney, M.W. Crowder, "Spectroscopic and biochemical characterization of metallo-b-lactamase IMP-1 with dicarboxylic, sulfonyl, and thiol inhibitors" Bioorg. Med. Chem. (2021) 40 1161-83
117. Z. Cheng , C.R. Bethel , P.W. Thomas , B.A. Shurina , J.P. Alao , C.A. Thomas , K. Yang , S.H Marshall , H. Zhang , A.M. Sturgill , A.N. Kravats , R.C. Page , W. Fast , R.A. Bonomo , M.W. Crowder, "Carbapenem use is driving the evolution of impenemase" Antimicro. Agents Chemo. (2021) 65 (4) e01714-20.
Publications (2016-2020)
116. K. Yang, A. Somogyi, C. Thomas, H. Zhang, Z. Cheng, S. Xu, C. Miller, D. Spivey, C. Blake, Clay Smith, D. Dafoe, N.D. Danielson, M.W. Crowder, "Analysis of barrel-aged Kentucky bourbon whiskey by ultrahigh resolution mass spectrometry" Food Analyt. Meth. (2020) 13 (12) 2301-2311.
115. M.R. Mehaffey, Y.C. Ahn, D.D. Rivera, P.W. Thomas, Z. Cheng, M.W. Crowder, R. Pratt, W. Fast, J. Brodbelt, “Elusive Structural Changes of New Delhi Metallo-β-Lactamase Revealed by Ultraviolet Photodissociation Mass Spectrometry” Chemical Science (2020) 11 8999-9010.
114. Caitlyn A. Thomas, Zishuo Cheng, Kundi Yang, Elle Hellwarth, Cole J. Yurkiewicz, Faith M. Baxter, Sarah A. Fullington, Spencer A. Klinsky, Jen L. Otto, Allie Y. Chen, Seth M. Cohen, Michael W. Crowder “Probing the mechanisms of inhibition for various inhibitors of metallo-b-lactamases VIM-2 and NDM-1” J. Inorg. Biochem. (2020) 210 111-123.
113. Sarah Fullington, Zishuo Cheng, Caitlyn Thomas, Callie Miller, Kundi Yang, Lin-Cheng Ju, Alexander Bergstrom, Ben A. Shurina, Stacey Lowery Bretz, Richard C. Page, David L. Tierney, and Michael W. Crowder, "An Integrated biophysical approach to discovering mechanisms of NDM-1 inhibition for several thiol-containing drugs" J. Biol. Inorg. Chem. (2020) 25 717-727.
112. A.Y. Chen, C. Thomas, P.W. Thomas, K. Yang, Z. Cheng, W. Fast, M.W. Crowder, S.M. Cohen, "Iminodiacetic acid as a novel metal-binding pharmacophore for New Delhi Metallo-b-lactamase Inhibitor Development" ChemMedChem (2020) 15 1272- 1282, http://dx.doi.org/10.1002/cmdc.202000123.
111. Zishuo Cheng, Caitlyn A. Thomas, Adam R. Joyner, Robert L. Kimble, Aidan M. Sturgill, Nhu-Y Tran, Maya R. Vulcan, Spencer A. Klinsky, Diego J. Orea, Cody R. Platt, Fanpu Cao, Bo Li, Qilin Yang, Cole J. Yurkiewicz, Walter L. Fast, and Michael W. Crowder, "MBLinhibitors.com, a website resoure offering information and expertise for the continued development of metallo-b-lactamase inhibitors" Biomolecules (2020) 10 (3) https://www.mdpi.com/2218-273X/10/3/459.
110. Zishuo Cheng, Ben Shurina, Christopher Bethel, Pei Thomas, Steven Marshall, Caitlyn Thomas, Kundi Yang, Robert Kimble, Jonathan Montgomery, Matthew Orischak, Callie Miller, Jordan Tennenbaum, Jay Nix, David Tierney, Walter Fast, Robert Bonomo, Richard Page, and Michael Crowder, "A single salt-bridge in VIM-20 increases protein stability and antibiotic resistance in low zinc conditions" mBio (2019) 10 (6) e02412-19 doi: 10.1128/mBio.02412-19.
109. A.Y. Chen, P.W. Thomas, Z. Cheng, N.Y. Xu, D.L. Tierney, M.W. Crowder, W. Fast, S.M. Cohen, "Investigation of dipicolinic acid isoteres for the inhibition of metallo-b-lactamases" ChemMedChem (2019) 14 1271-1282.
108. Z. Cheng, J. VanPelt, A. Bergstrom, C. Bethel, A. Katko, C. Miller, K. Mason, E. Cumming, H. Zhang, R.L. Kimble, S. Fullington, S.L. Bretz, J.C. Nix, R.A. Bonomo, D.L. Tierney, R.C. Page, M.W Crowder, "A non-canonical metal center drives activity of the Sediminispirochaeta smaragdinae metallo-β-lactamase SPS-1" Biochemistry (2018) 57 (35) 5218-5229.
107. Z. Cheng, P.W. Thomas, L. Ju, A. Bergstrom, K. Mason, D. Clayton, C. Miller, C.R. Bethel, J, VanPelt, D.L. Tierney, R. Page, R.A. Bonomo, W. Fast, M.W. Crowder, "Evolution of New Delhi metallo-β-lactamase (NDM) in the clinic: Effects of NDM mutations on stability, zinc affinity, mono-zinc activity" J. Biol. Chem. (2018) 293(32) 12606-12618 (Editor's Pick).
106. L.C. Ju, Z. Cheng, W. Fast, R.A. Bonomo, M.W. Crowder, "The contining challege of metallo-b-lactamase inhibition: Mechanism matters" Trends Pharmacological Sci. (2018) 39 (7) 635-647.
105. A. Bergstrom, A. Katko, Z. Adkins, J. Hill, M. Burnett, H. Yang, R. Bonomo, R. Page, D. Tierney, W. Fast, G. Wright, M. Crowder, "Probing the interaction of Aspergillomarasmine A (AMA) with metallo-β-lactamases NDM-1, VIM-2, and IMP-7" ACS Infect. Dis. (2018) 4 (2) 135-145.
104. A. Stewart, C. Bethel, Z. Cheng, A. Bergstrom, C. Miller, J. VanPelt, C. Williams, R. Poth, M. Morris, O. Lahey, J. Nix, D. Tierney, R. Page, M. Crowder, R. Bonomo, W. Fast, "Clinical Variants of New Delhi Metallo-β-Lactamase Are Evolving to Overcome Zinc Scarcity" ACS Infect. Dis. (2017) 3 (12) 927-940.
103. W. Craig, T. Baker, A. Marts, D. DeGenova, D. Martin, G. Reed, R. McCarrick, M. Crowder, S. Cohen, D. Tierney, "Substituent effects on the coordination chemistry of metal-binding pharmacophores" Inorg. Chem. (2017) 56 (19) 11721-11728.
102. A. Chen, P. Thomas, A. Stewart, A. Bergstrom, Z. Cheng, C. Miller, C. Bethel, S. Marshall, C. Credille, C. Riley, R. Page, R. Bonomo, M. Crowder, D. Tierney, W. Fast, S. Cohen, "Dipicolinic acid derivatives as inhibitors of New Delhi metallo-b-lactamase-1" J. Med. Chem. (2017) 60 (17) 7267-7283.
101. M.N. Lisa, A.R. Palacios, M. Aitha, M.M. Gonzaelz, D.M. Moreno, M.W. Crowder, R.A. Bonomo, J. Spencer, D.L. Tierney, L.I. Llarrull, A.J. Vila, "A general reaction mechanism for carbapenem hydrolysis by mono- and binuclear metallo-b-lactamases" Nature Comm. (2017) 8 (1) 538.
100. R. Muthyala, W.S. Shin, Y.Y. Sham, A. Bergstrom, M.W. Crowder, R.A. Bonomo, "Discovery of 1-hydroxypyridine-2(1H)-thione-6-carboxylic acid as a first-in-class low cytotoxic nanomolar metallo-b-lactamase inhibitor" ChemMedChem (2017) 12, 1-6.
99. F. Meng, H. Yang, C. Jack, H. Zhang, A. Moller, D. Spivey, R.C. Page, D.L. Tierney, and M.W. Crowder, "Biochemical characterization and zinc binding group (ZBGs) inhibition studies on the catalytic domain of MMP7 (cdMMP7)" J. Inorg. Biochem. (2016) 165 7-17.
98. F. Meng, H. Yang, M. Aitha, S. George, D.L. Tierney, and M.W. Crowder, "Biochemical and spectroscopic characterization of the catalytic domain of MMP16 (cdMMP16)" J. Biol. Inorg. Chem. (2016) 21 (4) 523-535.
97. M. Aitha, D.L. Tierney, and M.W. Crowder, "Probing substrate binding to the metal binding sites in metallo-b-lactamase L1 during catalysis" MedChemComm (2016) 7 194-201.
96. M. Aitha, A.J. Moller, I.D. Sahu, M. Horitani, D.L. Tierney, and M.W. Crowder, "Investigating the position of the hairpin loop in New Delhi metallo-b-lactamase, NDM-1, during catalysis and inhibitor binding" J. Inorg. Biochem. (2016) 156 35-39.
Publications (2011-2015)
95. H. Yang and M.W. Crowder, "New Delhi metallo-β-lactamase (NDM)" The Biochemist (2015) June edition, 18-21.
94. H. Yang, K. Makaroff, N. Paz, M. Aitha, M.W. Crowder, and D.L. Tierney, "Metal ion dependence of the matrix metalloproteinase-1" Biochemistry (2015) 54 (23) 3631-3639 .
93. T. Richmond, J. Buell, S. Pfeil, and M.W. Crowder, "Evidence-based recommendations for maximizing competitive swimming performance" J. Swimming Res. (2015) 25 (1) 1-15.
92. P. Oelschlaeger, M. Aitha, H. Yang, J. Kang, A. Zhang, E. Liu, J. Buynak, and M. Crowder, "Meropenem and chromacef intermediates observed in IMP-25 metallo-β-lactamase-catalyzed hydrolysis" Antimicro. Agents Chemo. (2015) 59 4326-4330.
91. M. Aitha, L. Moritz, I.D. Sahu, O. Sanyurah, Z. Roche, R. McCarrick, G.A. Lorigan, B. Bennett, and M.W. Crowder, "Conformational dynamics of metallo-β-lactamase CcrA during catalysis investigated by using DEER spectroscopy" J. Biol. Inorg. Chem. (2015) 20 (3) 585-594.
90. M. Aitha, A. Marts, A. Bergstrom, A. Moller, L. Moritz, L. Turner, J. Nix, R. Bonomo, R. Page, D.L. Tierney, M.W. Crowder, "Biochemical, mechanistic, and spectroscopic characterization of metallo-β-lactamase VIM-2" Biochemistry (2014) 53 (46) 7321-7331.
89. H. Yang, H. Young, L. Sutton, and M.W. Crowder, "Targeting metallo-carbapenemases via modulation of electronic properties of cephalosporins" Biochem. J. (2014) 464 (2) 271-279.
88. Y.L. Zhang, K.W. Yang, Y.J. Zhou, A.E. LaCuran, P. Oelschlaeger, and M.W. Crowder, "Diaryl-substituted azolthioacetamides: inhibitor discovery of New Delhi metallo-β-lactamase-1 (NDM-1)" ChemMedChem (2014) 9 (11) 2445-2448.
87. H. Yang, M. Aitha, A.R. Marts, A. Hetrick, B. Bennett, M.W. Crowder, and D.L. Tierney, "Spectroscopic and mechanistic studies of heterodimetallic forms of metallo-β-lactamase NDM-1" J. Am. Chem. Soc. (2014) 136 (20) 7273-7285.
86. M. Aitha, T.K. Richmond, Z. Hu, A. Hetrick, R. Reese, A. Gunther, R. McCarrick, B. Bennett, and M.W. Crowder, "Dilution of dipolar interactions in a spin-labeled, multimeric metalloenzyme for DEER studies" J. Inorg. Biochem. (2014) 136 40-46.
85. K.W. Yang, L. Fang, S.K. Yang, M. Aitha, A.E. LaCuran, P. Oelschlaeger, M.W. Crowder, “New β-phospholactam as a carbapenem transition state analog: synthesis of a broad-spectrum inhibitor of metallo-β-lactamases” Bioorg. Med. Chem. Lett. (2013) 23 (21) 5855-5859.
84. H. Heidari Torkabadi, T. Che, J. Shou, S. Shanmugam, M. Crowder, R. Bonomo, M. Pusztai-Carey, P. Carey, “Raman spectra of interchanging β-lactamase inhibitor intermediates on the millisecond time scale” J. Am. Chem. Soc. (2013) 135 (8) 2895-2898.
83. Y.L. Zhang, J.M. Xiao, J.L. Feng, K.W. Yang, L. Feng, L.S. Zhou, M.W. Crowder, “A novel fluorogenic substrate for dinuclear Zn(II)-containing metallo-β-lactamases ” Bioorg. Med. Chem. Lett. (2013) 23 (6) 1676-1679.
82. L.S. Zhou, K.W. Yang, L. Feng, J.M. Xiao, C.C. Liu, Y.L. Zhang, M.W. Crowder, “Novel fluorescent risedronates: synthesis, photodynamic inactivation, and imaging of Bacillus subtilis” Bioorg. Med. Chem. Lett. (2013) 23 (4) 949-954.
81. L. Feng, K.W. Yang, L.S. Zhou, J.M. Xiao, X. Yang, L. Zhai, Y.L. Zhang, and M.W. Crowder, “N-heterocyclic dicarboxylic acids: broad-spectrum inhibitors of metallo-β-lactamases with co-antibacterial effect against antibiotic resistant bacteria” (2012) Bioorg. Med. Chem. Lett. 22 (16) 5185-5189.
80. H. Yang, M. Aitha, A. Hetrick, T.K. Richmond, D.L. Tierney, and M.W. Crowder, “Mechanistic and spectroscopic studies on metallo-β-lactamase NDM-1” Biochemistry (2012) 51 3839-3847.
79. M.P. Hensley, T.S. Gunasekera, J.A. Easton, T.K. Sigdel, S.A. Sugarbaker, L. Klingbeil, R.M. Breece, D.L. Tierney, and M.W. Crowder, “Characterization of Zn(II)-responsive proteins YkgM and L31 in E. coli” J. Inorg. Biochem. (2012) 111 164-172.
78. C. Luxford, M.W. Crowder, and S.L. Bretz, “A symmetry POGIL activity for inorganic chemistry” J. Chem. Ed. (2012) 89(2) 211-214.
77. D. Griffin, T.K. Richmond, C.Sanchez-Geraci, A. Moller, R.M. Breece, D.L. Tierney, B. Bennett, and M.W. Crowder, “Structural and kinetic studies on metallo-β-lactamase IMP-1” Biochemistry (2011) 50 9125-9134.
76. M.P. Hensley, D.L. Tierney, and M.W. Crowder, “Zn(II) binding to E. coli 70S ribosomes” (2011) Biochemistry 50 9937-39.
75. K. Linenberger, S.L. Bretz, M.W. Crowder, R. McCarrick, D.L. Tierney, and G.A. Lorigan, “What is fresh meat’s true color? Investigating the effects of ligand binding on myoglobin using optical, electron paramagnetic resonance, and nuclear magnetic resonance spectroscopy” J. Chem. Ed. (2011) 88 223-225.
Publications (2006-2010)
74. P. Limphong, N.E. Adams, M. Rouhier, R. McKinney, M. Naylor, B. Bennett, C.A. Makaroff, and M.W. Crowder, “Converting GLX2-1 into an active glyoxalase II” Biochemistry (2010) 49 8228-8236.
73. P. Limphong, R.M. McKinney, N.E. Adams, C.A. Makaroff, B. Bennett, and M.W. Crowder, “On the metal ion requirements of A. thaliana Glx2-2 for catalytic activity” J. Biol. Inorg. Chem. (2010) 15 (2) 249-258.
72. P. Limphong, G. Nimako, P. Thomas, W. Fast, C.A. Makaroff, and M.W. Crowder, “Arabidopsis thaliana mitochondrial glyoxalase 2-1 exhibits β-lactamase activity” Biochemistry (2009) 48 8491-8493.
71. T.S. Gunasekera, A.H. Herre, and M.W. Crowder, “Absence of ZnuABC-mediated zinc uptake affects virulence-associated phenotypes of uropathogenic Escherichia coli CFT073 under Zn(II)-depleted conditions” FEMS Microbiol. Lett. (2009) 300 36-41.
70. R.M. Breece, Z. Hu, B. Bennett, M.W. Crowder, and D.L. Tierney, “Motion of the zinc ions in catalysis by a di-zinc metallo-β-lactamase” J. Am. Chem. Soc. (2009) 131 11642-11643.
69. M. Hawk, R. Breece, C. Hajdin, K. Bender, Z. Hu, A. Costello, B. Bennett, D. Tierney, M. Crowder, “Differential binding of Co(II) and Zn(II) to metallo-β-lactamase Bla2 from Bacillus anthracis” J. Am. Chem. Soc. (2009) 131 10753-10762.
68. P. Limphong, R. McKinney, N. Adams, B. Bennett, C.A. Makaroff, T. Gunasekera, and M.W. Crowder, “Human Glx2 contains an Fe(II)Zn(II) center but is active as a mononuclear Zn(II) enzyme” Biochemistry (2009) 48 5426-5434.
67. Z. Hu, L. Spadafora, C. Hajdin, B. Bennett, and M.W. Crowder, “Structure and mechanism of Cu- and Ni-substituted analogs of metallo-β-lactamase L1” Biochemistry (2009) 48 2981-2989.
66. F.R. Salsbury Jr., M.W. Crowder, S.F. Kingsmore, and J.A. Huntley, “Molecular dynamic simulations of the metallo-β-lactamase from Bacteroides fragilis in presence and absence of a tight-binding inhibitor” J. Mol. Modeling. (2009) 15 133-145.
65. Gunasekera, T.S., Easton, J.A., Sugarbaker, S.A., Klingbeil, L., and Crowder, M.W., “Zn(II) homeostasis in E. coli” in Advances in Organic Chemistry: Cellular Systems and Synthetic Models – ACS Symposium Series 1012, (2009) Oxford University Press, Ch. 6. Ed. M.J. Baldwin and E. Long. pp 81-95.
64. P. Limphong, M.W. Crowder, B. Bennett, and C.A. Makaroff, “Arabidopsis thaliana GLX2-1 contains a dinuclear metal binding site but is not a glyoxalase 2” Biochem. J. (2009) 417 323-330.
63. Hu, Z., Periyannan, G., Bennett, B., and Crowder, M.W., “Role of the Zn1 and Zn2 sites in metallo-β-lactamase L1” J. Am. Chem. Soc. (2008) 130 (43) 14207-14216.
62. Hu, Zhenxin, Gunasekera, T.S., Spadafora, L., Bennett, B., and Crowder, M.W., “Metal content of metallo-β-lactamase L1 is determined by the bioavailability of metal ions” Biochemistry (2008) 47 7947-7953.
61. M.M. Holdorf, B. Bennett, M.W. Crowder, and C.A. Makaroff, “Spectroscopic studies on Arabidopsis ETHE1, a glyoxalase II-like protein” J. Inorg. Biochem. (2008) 102 1825-1830.
60. Sharma, N., Hu, Z., Crowder, M.W., and Bennett, B. “Conformational Changes in the Metallo-β-lactamase ImiS During the Catalytic Reaction: an EPR Spectrokinetic Study of Co(II)- Spin Label Interactions” J. Am. Chem. Soc. (2008) 130 8215-8222.
59. Z. Hu, G. Periyannan, and M.W. Crowder, “Folding strategy to prepare Co(II)-substituted metallo-β-lactamase L1” Anal. Biochem. (2008) 378 177-183.
58. L.A. Yatsunyk, J.A. Easton, L.R. Kim, S.A. Sugarbaker, B. Bennett, R.M. Breece, I.I. Vorontsov, D.L. Tierney, M.W. Crowder, and A.C. Rosenzweig, “Structural and metal binding properties of ZnuA, a periplasmic zinc transporter from Escherichia coli” J. Biol. Inorg. Chem. (2008) 13 271-288.
57. J. Crisp, R. Conners, J.D. Garrity, A.L. Carenbauer, M.W. Crowder, and J. Spencer, “A structural basis for the role of Asp-120 in metallo-β-lactamases” Biochemistry (2007) 46 10664-10674.
56. J.A. Easton, P. Thompson, and M.W. Crowder, “Time-dependent Translational Response of E. coli to Excess Zn(II)” J. Biomol. Tech. (2006) 17 303-307.
55. A.L. Costello, N.P. Sharma, K.W. Yang, M.W. Crowder, and D.L. Tierney, “EXAFS of the Zinc Binding Sites in the Class B2 Metallo-β-Lactamase ImiS from Aeromonas veronii bv. sobria” Biochemistry (2006) 45 13650-13658.
54. M.L. Matthews, G. Periyannan, T.K. Sigdel, C. Hajdin, B. Bennett, and M.W. Crowder, “Probing the reaction mechanism of the D-ala-D-ala dipeptidase, VanX, by using stopped-flow kinetic and rapid-freeze quench EPR studies on the Co(II)-substituted enzyme” J. Am. Chem. Soc. (2006) 128 13050-13051.
53. Q. Sun, A. Law, M.W. Crowder, and H.M. Geysen, “Homo-cysteinyl peptide inhibitors of the L1 metallo-β-lactamase, and SAR as determined by combinatorial library synthesis” Bioorg. Med. Chem. Lett. (2006) 16 5169-5175.
52. N.P. Sharma, C. Hajdin, S. Chandrasekar, B. Bennett, and M.W. Crowder, “Mechanistic studies on the mononuclear Zn(II)-containing metallo-β-lactamase ImiS from Aeromonas sobria” Biochemistry (2006) 45 10729-10738.
51. T.K. Sigdel, J.A. Easton, and M.W. Crowder, “Transcriptional response of E. coli to TPEN” J. Bacteriol. (2006) 188 6709-6713.
50. M.W. Crowder, J. Spencer, and A. Vila, "Metallo-β-lactamases: Novel weaponry for Antibiotic Resistance in Bacteria" Acc. Chem. Res. (2006) 39 721-728.
49. M.W. Crowder, "Combating Vancomyin Resistance in Bacteria: Targeting the D-ala-D-ala Dipeptidase VanX" Curr. Drug Targets - Infect. Disorders. (2006) 6 (2) 147-158.
48. A. L. Costello, G. Periyannan, K.-W. Yang, M. W. Crowder and D. L. Tierney, "Site Selective Binding of Zn(II) to Metallo-β-Lactamase L1 from Stenotrophomonas maltophilia" J. Biol. Inorg. Chem. (2006) 11 351-358.
47. T.K. Sigdel, , R. Cilliers, P.R. Gursahaney, P. Thompson, J.A. Easton, and M.W. Crowder, "Proteomic analysis of Zn(II) responsive proteins in E. coli" Biometals (2006) 19 461-471.
46. F.C. Golich, M. Han, and M.W. Crowder, "Over-expression, Purification, and Characterization of Aminopeptidase N (PepN) from Escherichia coli" Prot. Express. Purif. (2006) 47 634-639.
45. G.R. Periyannan, A.L. Costello, D.L. Tierney, B. Bennett, K.W. Yang, and M.W. Crowder, "Sequential Binding of Co(II) to Metallo-β-Lactamase CcrA" Biochemistry (2006) 45 1313-1320.
Publications (2001-2005)
44. G.P.K. Marasinghe, I.M. Sander, Brian Bennett, Ke-Wu Yang, Gopalraj Periyannan, C.A. Makaroff, and M.W. Crowder, "Structural Studies on a Mitochondrial Glyoxalase II" J. Biol. Chem. (2005) 280 40668-40675.
43. Ke-Wu Yang, F.C. Golich, T.K. Sigdel, and M.W. Crowder, "Phosphinate, Sulfonate, and Sulfonamidate Dipeptides as Potential Inhibitors of E. coli Aminopeptidase N" Bioorg. Med. Chem. Lett. (2005) 15 5150 - 5153.
42. P.A. Crawford, K.W. Yang, N. Sharma, B. Bennett, and M.W. Crowder "Spectroscopic Studies on Co(II)-Substituted Metallo-β-Lactamase ImiS from Aeromonas veronii bv. sobria" Biochemistry (2005) 44 5168 - 5176.
41. R.M. Breece, A. Costello, B. Bennett, T.K. Sigdel, M.L. Matthews, D.L. Tierney, and M.W. Crowder, "A five-coordinate metal center in Co(II)-substituted VanX" J. Biol. Chem. (2005) 280 11074-11081.
40. J.D. Garrity, B. Bennett, and M.W. Crowder, "Direct Evidence that Reaction Intermediate in Metallo-β-lactamase L1 is Metal Bound" Biochemistry (2005) 44 1078-1087.
39. J.D. Garrity, J.M. Pauff, and M.W. Crowder, "Probing the dynamics of a mobile loop above the active site of L1, a metallo-β-lactamase from Stenotrophomonas maltophilia, via site-directed mutagenesis and stopped-flow fluorescence spectroscopy" J. Biol. Chem. (2004) 279 39663-39670.
38. Tara K. Sigdel, Renee Cilliers, Priya R. Gursahaney, and Michael W. Crowder, "Fractionation of soluble proteins in E. coli using DEAE-, SP-, and Phenyl Sepharose Chromatographies" J. Biomol. Tech. (2004) 15 199-207.
37. Gopalraj Periyannan, Patrick J. Shaw, Tara Sigdel, and Michael W. Crowder, "In vivo Folding of Recombinant Metallo-β-Lactamase L1 Requires the Presence of Zn(II)" Prot. Sci. (2004) 13 2236-2243.
36. F.C. Golich, T. Sigdel, R.M. Breece, and M.W. Crowder, "L-Alanine-p-nitroanilide is not a Substrate for VanX" Anal. Biochem. (2004) 331 398-400.
35. P.A. Crawford, N. Sharma, S. Chandrasekar, T. Sigdel, T.R. Walsh, J. Spencer, and M.W. Crowder, "Over-expression, Purification, and Characterization of Metallo-β-Lactamase ImiS from Aeromonas veronii bv. sobria" Prot. Express. Purif. (2004) 36 272-279.
34. K.W. Yang and M.W. Crowder, "A Method for Removing EDTA from Apo-proteins" Anal. Biochem. (2004) 329 342-344.
33. N.F. Wenzel, A.L. Carenbauer, M.P. Pfeister, O. Schilling, A. Vogel, W. Meyer-Klaucke, C.A. Makaroff, and M.W. Crowder, "The Binding of Iron and Zinc to Glyoxalase II occurs exclusively as Di-metal Centers and is Unique within the Metallo-β-Lactamase Family" J. Biol. Inorg. Chem. (2004) 9 429 - 438.
32. J.D. Garrity, A.L. Carenbauer, L.R. Herron, and M.W. Crowder, "Metal binding Asp120 in metallo-β-lacatamse L1 from Stenotrophomonas maltophilia plays a crucial role in catalysis" J. Biol. Chem. (2004) 279 920-927.
31. X. Cao, A. Iqbal, A. Patel, P. Gretz, G. Huang, M. Crowder, and R.A. Day, "3-Alkoxy-5-isoxazolidinones mimic β-lactams" Biochem. Biophys. Res. Comm. (2003) 311 267-271.
30. O. Schilling, N. Wenzel, M. Naylor, A. Vogel, M. Crowder, C. Makaroff, and W. Meyer-Klaucke, "New Insights into the metal selectivity of the metallo-β-lactamase domain: glyoxalase II binds iron, manganese, and zinc in vivo at various ratios with equal catalytic activities" Biochemistry (2003) 42 11777-11786.
29. K.W. Yang, D.N. Sobieski, A.L. Carenbauer, P.A. Crawford, C.A. Makaroff, and M.W. Crowder, "Explaining the Inhibition of Glyoxalase II by FMOC-Protected Glutathione Derivatives" Arch. Biochem. Biophys. (2003) 414 271-278.
28. L. Jacquamet, W. Gu, Y. Sun, M.W. Crowder, G.A. Lorigan, J.M. Latour, S.P. Cramer, and J.B. Vincent, "Characterization of Chromodulin by X-ray Absorption and Electron Paramagnetic Resonance Spectroscopy and Magnetic Susceptibility Measurements" J. Am. Chem. Soc. (2003) 125 774-780.
27. C.S. Higgins, A.M. Simm, A.L. Carenbauer, M.W. Crowder, J.H. Bateson, P.M. Bennett, and T.R. Walsh, "The N-Terminus of the Stenotrophomonas maltophilia L1 Metallo-β-Lactamase Influences Substrate Binding and Hydrolytic Activity" J. Biol. Chem. (2002) 277 24744-24752.
26. A.L. Carenbauer, J. Garrity, G. Periyannan, R. Yates, and M.W. Crowder, "Probing Substrate Binding to Metallo-β-Lactamase L1 by Using Site Directed Mutagenesis" BMC Biochemistry (2002) 3 4.
25. T.M. Zang, D.A. Hollman, P.A. Crawford, M.W. Crowder, and C.A. Makaroff, "Arabidopsis Glyoxalase II Contains A Zinc/Iron Binuclear Metal Center That Is Essential For Substrate Binding And Catalysis" J. Biol. Chem. (2001) 276 4788-4795.
24. M.W. Crowder, K.W. Yang, A.L. Carenbauer, G. Periyannan, M.E. Seifert, N.E. Rude, T.R. Walsh, "The Problem of a Solvent Exposable Disulfide when Preparing Co(II)-Substituted Metallo-β-Lactamase L1 from Stenotrophomonas maltophilia" J. Biol. Inorg. Chem. (2001) 6 (1) 91-99.
Publications (1997-2000)
23. M.E. Yanchak, R.A. Taylor, and M.W. Crowder, "Mutational Analysis of Metallo-β-Lactamase CcrA from Bacteroides fragilis" Biochemistry (2000) 39 11330-11339.
22. J.J. Brandt, L.L. Chatwood, and M.W. Crowder, "Analysis of Three Overexpression Systems for VanX, the Zn(II)-Dipeptidase Required for High-Level Vancomycin Resistance in Bacteria" Prot. Express. Purif. (2000) 20 300-307.
21. K.W. Yang, J.J. Brandt, L.L. Chatwood, and M.W. Crowder, "Phosphonamidate and Phosphothioate Dipeptides as Potential Inhibitors of VanX" Bioorg. Med. Chem. Lett. (2000) 10 1087-1089.
20. J.K. Speetjens, A. Parand, M.W. Crowder, J.B. Vincent, and S.A. Woski, "Low Molecular Weight Chromium Binding Substance and Biomimetic [Cr3O(O2CCH2CH3)6(H2O)3]+ Do Not Cleave DNA Under Physiologically-Relevant Conditions" Polyhedron (1999) 18 2617-2624.
19. J.J. Brandt, L.L. Chatwood, K.W. Yang, and M.W. Crowder, "Continuous Assay for VanX, the D-Ala-D-Ala Dipeptidase Required for High-Level Vancomycin Resistance" Anal. Biochem. 272 (1999) 94-99.
18. K.W. Yang and M.W. Crowder, "Inhibition Studies on Metallo-β-Lactamase L1 from Stenotrophomonas maltophilia" Arch. Biochem. Biophys. 368 (1999) 1-6.
17. M.W. Crowder and T.R. Walsh, "Structure and Function of Metallo-β-Lactamases" Research Signpost (1999) 3 105-132.
16. S. McManus-Munoz and M.W. Crowder, "Kinetic Mechanism of Metallo-β-Lactamase L1 from Stenotrophomonas maltophila" Biochemistry 38 (1999) 1547-1553.
15. M.W. Crowder, A. Numan, F. Haddadian, M.A. Weitzel, and N.D. Danielson, "Capillary Electrophoresis of Phosphoamino Acids with Indirect Photometric Detection" Analyt. Chim. Acta 384 (1999) 127-133.
14. M.W. Crowder, T.R. Walsh, L. Banovic, M. Pettit, and J. Spencer, "Overexpression, Purification, and Characterization of the Cloned Metallo-β-Lactamase (L1) from Stenotrophomonas maltophilia" Antimicro. Agents Chemo. 42 (1998) 921-926.
13. A. Parand, A.C. Royer, T.L. Cantrell, M. Weitzel, N. Memon, J.B. Vincent, and M.W. Crowder, "DNA Nicking by a Trinuclear Chromium Complex" Inorg. Chim. Acta 268 (1998) 211-219.
12. M.W. Crowder, M.K. Maiti, L. Banovic, and C.A. Makaroff, "Glyoxalase II from A. thaliana Requires Zn(II) for Catalytic Activity" FEBS Lett. 418 (1997) 351-354.
Publications before Miami University
11. M.W. Crowder, Z.Wang, S.L. Franklin, E.P. Zovinka, and S.J. Benkovic, " Beta-Lactamase from B. fragilis Contains Two Moles of Tightly Bound Zn(II)" Biochemistry 35 (1996) 12126-12132.
10. Phosphatases in Cell Metabolism and Signal Transduction Michael W. Crowder and John B. Vincent, R.G. Landes Biomedical Publishers, 1995.
9. M. W. Crowder, J.D. Stewart, V.A. Roberts, C.J. Bender, E. Tevelrach, J. Peisach, E.D. Getzoff, B.J. Gaffney, and S. J. Benkovic, " Spectroscopic Studies on Metal Binding Antibody 43C9" J. Am. Chem. Soc. 117 (1995) 5627-5634.
8. J.D. Stewart, V.A. Roberts, M.W. Crowder, E.D. Getzoff, and S.J. Benkovic, "Creation of a Novel Biosensor for Zinc" J. Amer. Chem. Soc. 116 (1994) 415-416.
7. E.G. Mueller, M. W. Crowder, B.A. Averill, and J.M. Knowles, "Purple Acid Phosphatase: A Diiron Enzyme that Catalyzes a Direct Phospho Group Transfer to Water" J. Am. Chem. Soc. 115 (1993) 2974-2975.
6. M. W. Crowder, J.B. Vincent, and B.A. Averill, "Electron Paramagnetic Resonance Studies on the High-Salt Form of Bovine Spleen Purple Acid Phosphatase" Biochemistry 31 (1992) 9603-9608.
5. Z. Wang, L.-J. Ming, L. Que Jr., J. B. Vincent, M. W. Crowder, and B.A. Averill, "1H NMR Studies of Bovine Spleen Purple Acid Phosphatase and its Anion Complexes" Biochemistry 31 (1992) 5263-5268.
4. J.B. Vincent, M.W. Crowder, and B.A. Averill, " Purple Acid Phosphatases: Multiple Binding Sites for Tetrahedral Oxyanion Inhibitors" Biochemistry 31 (1992) 3033-3037.
3. J.B. Vincent, M.W. Crowder, and B.A. Averill, "Hydrolysis of Phosphate Monoesters: A Biological Problem with Multiple Chemical Solutions" Trends Biochem. Sci. 17 (1992) 105-113.
2. J.B Vincent, M.W. Crowder, and B.A. Averill, "Evidence for a Phosphoryl-Enzyme Intermediate in Phosphate Ester Hydrolysis by Purple Acid Phosphatase from Bovine Spleen" J. Biol. Chem. 266 (1991) 17737-17740.
1. J.B. Vincent, M.W. Crowder, and B.A. Averill, "Spectroscopic and Kinetics Studies of a High-Salt Stabilized Form of the Purple Acid Phosphatase from Bovine Spleen," Biochemistry 30 (1991) 3025-3034